LOCUS       sequence-259573-        9043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of human miR-302 cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Peskova L, Cerna K, Oppelt J, Mraz M, Barta T
  TITLE     Oct4-mediated reprogramming induces embryonic-like microRNA 
            expression signatures in human fibroblasts.
  JOURNAL   Sci Rep. 2019 Oct 31;9(1):15759. doi: 10.1038/s41598-019-52294-3.
  PUBMED    31673026
REFERENCE   2  (bases 1 to 9043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-10-31"; volume: "9(1):15759. doi"; pages: " 
            10.1038/s41598-019-52294-3"
COMMENT     Sequence Label: pCW57-GFP-miR-302cluster
FEATURES             Location/Qualifiers
     source          1..9043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..320
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    157..175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    228..246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     293..317
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      343..352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             349..1044
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     promoter        1779..2289
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2208..2226
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2299..2895
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2299..2318)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2795..2815
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2896..2949
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2950..3567,3568..3573,3574..3609,3610..3612,3613..3648,
                     3649..3651,3652..3687,3688..3696)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3736..3756)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4154..4165)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4370..4603
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4675..4796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4712..4731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4766..4785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4836..4971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4971..5042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5043..5111,5112..5903)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5261..5280)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6074..6662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6563..6582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6816..6833
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6950..6971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6986..6991,6992..7009,7010..7016)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7024..7040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7029..7051
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7048..7064
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7048..7064
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7083..7103
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7085..7103
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7131..7357
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7358..7538
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7585..7710
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8203..8436
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8621..8665
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8878..8995
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
       61 gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
      121 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      181 tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      241 tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      301 tagtgaaccg tcagatcgcc tggagaattg gctagcgaat tcgccaccat ggagagcgac
      361 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
      421 gagttcgagc tggtgggcgg cggagagggc acccccgagc agggccgcat gaccaacaag
      481 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
      541 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
      601 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
      661 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtgatg
      721 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
      781 accgtggagc acctgcaccc catgggcgat aacgatctgg atggcagctt cacccgcacc
      841 ttcagcctgc gcgacggcgg ctactacagc tccgtggtgg acagccacat gcacttcaag
      901 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
      961 gaggaggatc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     1021 ccggatgcag atgccggtga agaaaccggt gaagttgtat gttggggtgg gctcccttca
     1081 actttaacat ggaagtgctt tctgtgactt taaaagtaag tgcttccatg ttttagtagg
     1141 agtgaatcca atttacttct ccaaaataga acacgctaac ctcatttgaa gggatcccct
     1201 ttgctttaac atgggggtac ctgctgtgtg aaacaaaagt aagtgcttcc atgtttcagt
     1261 ggaggtgtct ccaagccagc acaccttttg ttacaaaatt tttttgttat tgtgttttaa
     1321 ggttactaag cttgttacag gttaaaggat tctaactttt tccaagactg ggctccccac
     1381 cacttaaacg tggatgtact tgctttgaaa ctaaagaagt aagtgcttcc atgttttggt
     1441 gatggtaagt cttcctttta catttttatt atttttttag aaaataactt tattgtattg
     1501 accgcagctc atatatttaa gctttatttt gtatttttac atctgttaag gggccccctc
     1561 tactttaaca tggaggcact tgctgtgaca tgacaaaaat aagtgcttcc atgtttgagt
     1621 gtggtggttc ctacctaatc agcaattgag ttaacgccca cactgtgtgc agttcttggc
     1681 tacaggccat tactgttgct aatatgcaac tctgttgaat ataaattgga attgcacttt
     1741 agcaatggtg atggattgaa taaacgtgta caggatccgg ggttggggtt gcgccttttc
     1801 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     1861 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     1921 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     1981 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     2041 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     2101 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     2161 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     2221 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     2281 tctccccagc aattcaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     2341 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     2401 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     2461 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     2521 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     2581 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     2641 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     2701 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     2761 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     2821 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     2881 cgcaagcccg gtgccgaagg tagaggttct ctcctcactt gtggtgatgt tgaagaaaac
     2941 cctggtccaa tgtctagact ggacaagagc aaagtcataa acggagctct ggaattactc
     3001 aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     3061 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     3121 gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac
     3181 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     3241 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     3301 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     3361 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     3421 agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc
     3481 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     3541 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta
     3601 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct
     3661 cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcg atccaagata
     3721 tcgtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3781 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3841 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3901 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3961 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4021 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4081 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4141 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4201 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4261 cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg tacctttaag accaatgact
     4321 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     4381 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     4441 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4501 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4561 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     4621 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     4681 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4741 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4801 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4861 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4921 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctttcaaata
     4981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5041 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5101 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5161 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5221 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5281 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5341 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5401 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5461 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5521 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5581 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5641 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5701 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5761 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5821 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5881 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5941 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6001 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6181 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6721 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6781 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6841 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6901 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6961 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7021 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7081 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     7141 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     7201 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     7261 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     7321 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     7381 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7441 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7501 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     7561 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     7621 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     7681 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     7741 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     7801 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     7861 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     7921 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     7981 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     8041 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     8101 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     8161 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     8221 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     8281 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     8341 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     8401 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     8461 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     8521 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     8581 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     8641 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     8701 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     8761 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     8821 cccaaccccg aggggacaat tctcgacctc gagacaaatg gcagtattca tccacaattt
     8881 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     8941 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     9001 ttattacagg gacagcagag atccactttg gccgcgaatc gat
//
