LOCUS       sequence-25959-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector, V5-Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-V5-LUC (w468-1)
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(117..158)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(170..269)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(288..311)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(319..346)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(478..521)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gatggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta
       61 ccggaatgcc aagctttttg caaaagccta gggactgaat tggttcccat ggatcccgta
      121 gaatcgagac cgaggagagg gttagggata ggcttacctt ccatggtgga gcctgctttt
      181 ttgtacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
      241 actatcagtc aaaataaaat cattatttgg ggcccgagct taagtaacta actaacagga
      301 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      361 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
      421 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
      481 aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tttgatgcct ggcagttccc
      541 tactctcgct tagtagttag acgtccccga gatccatgct agcggtaata cggttatcca
      601 ccagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
//
