LOCUS       sequence-25962-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for miRNA expression under CMV promoter and GFP
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR/pSM2(CMV) GFP (w513-1)
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(96..120)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(117..320)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(146..166)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggtggatcc tctagagtcg gtgtcttcta
       61 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
      121 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      181 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      241 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      301 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
      361 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
      421 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
      481 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
      541 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
      601 ccatttaccg taagttatgt aagcggaact cccaaggttc ctttaaagcc tgctttttgg
      661 tacaaagttg gcattattaa aaaagcattg cttattca
//
