LOCUS       sequence-259624-        7318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human beta-catenin full length in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Xing Y, Takemaru K, Liu J, Berndt JD, Zheng JJ, Moon RT, Xu W
  TITLE     Crystal structure of a full-length beta-catenin.
  JOURNAL   Structure. 2008 Mar;16(3):478-87. doi: 10.1016/j.str.2007.12.021.
  PUBMED    18334222
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.str.2007.12"; journalName: "Structure"; date: "2008-03"; 
            volume: "16"; issue: "3"; pages: "478-87"
COMMENT     Sequence Label: pGEX4T1-human beta-catenin full length
FEATURES             Location/Qualifiers
     source          1..7318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     62..78
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             74..601
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(94..111)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(94..110)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(103..125)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(703..708,709..724,725..731)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    739..755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             778..1431
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1389..1411
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1438..1455
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1456..1476
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1477..3822
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     primer_bind     complement(3888..3910)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4055..4073)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4141..4245
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4246..4314,4315..5106)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4464..4483)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5277..5865
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5766..5785
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6019..6036
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        6109..6186
                     /gene="lacI"
                     /label=lacI promoter
     CDS             6187..7269
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6206..6225)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(7318,1..5,6..23,24..30)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga
      121 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      181 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      241 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
      301 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta
      361 cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc
      421 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca
      481 ggaaggccag acgcgaatta tttttgatgg cgttggaatt agcttatcga ctgcacggtg
      541 caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta
      601 aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg
      661 ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg
      721 ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agtattcatg
      781 tcccctatac taggttattg gaaaattaag ggccttgtgc aacccactcg acttcttttg
      841 gaatatcttg aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg
      901 cgaaacaaaa agtttgaatt gggtttggag tttcccaatc ttccttatta tattgatggt
      961 gatgttaaat taacacagtc tatggccatc atacgttata tagctgacaa gcacaacatg
     1021 ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc ggttttggat
     1081 attagatacg gtgtttcgag aattgcatat agtaaagact ttgaaactct caaagttgat
     1141 tttcttagca agctacctga aatgctgaaa atgttcgaag atcgtttatg tcataaaaca
     1201 tatttaaatg gtgatcatgt aacccatcct gacttcatgt tgtatgacgc tcttgatgtt
     1261 gttttataca tggacccaat gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa
     1321 cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta tatagcatgg
     1381 cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa atcggatctg
     1441 gttccgcgtg gatccgaaaa cctgtatttt cagggcatgg ctactcaagc tgatttgatg
     1501 gagttggaca tggccatgga accagacaga aaagcggctg ttagtcactg gcagcaacag
     1561 tcttacctgg actctggaat ccattctggt gccactacca cagctccttc tctgagtggt
     1621 aaaggcaatc ctgaggaaga ggatgtggat acctcccaag tcctgtatga gtgggaacag
     1681 ggattttctc agtccttcac tcaagaacaa gtagctgata ttgatggaca gtatgcaatg
     1741 actcgagctc agagggtacg agctgctatg ttccctgaga cattagatga gggcatgcag
     1801 atcccatcta cacagtttga tgctgctcat cccactaatg tccagcgttt ggctgaacca
     1861 tcacagatgc tgaaacatgc agttgtaaac ttgattaact atcaagatga tgcagaactt
     1921 gccacacgtg caatccctga actgacaaaa ctgctaaatg acgaggacca ggtggtggtt
     1981 aataaggctg cagttatggt ccatcagctt tctaaaaagg aagcttccag acacgctatc
     2041 atgcgttctc ctcagatggt gtctgctatt gtacgtacca tgcagaatac aaatgatgta
     2101 gaaacagctc gttgtaccgc tgggaccttg cataaccttt cccatcatcg tgagggctta
     2161 ctggccatct ttaagtctgg aggcattcct gccctggtga aaatgcttgg ttcaccagtg
     2221 gattctgtgt tgttttatgc cattacaact ctccacaacc ttttattaca tcaagaagga
     2281 gctaaaatgg cagtgcgttt agctggtggg ctgcagaaaa tggttgcctt gctcaacaaa
     2341 acaaatgtta aattcttggc tattacgaca gactgccttc aaattttagc ttatggcaac
     2401 caagaaagca agctcatcat actggctagt ggtggacccc aagctttagt aaatataatg
     2461 aggacctata cttacgaaaa actactgtgg accacaagca gagtgctgaa ggtgctatct
     2521 gtctgctcta gtaataagcc ggctattgta gaagctggtg gaatgcaagc tttaggactt
     2581 cacctgacag atccaagtca acgtcttgtt cagaactgtc tttggactct caggaatctt
     2641 tcagatgctg caactaaaca ggaagggatg gaaggtctcc ttgggactct tgttcagctt
     2701 ctgggttcag atgatataaa tgtggtcacc tgtgcagctg gaattctttc taacctcact
     2761 tgcaataatt ataagaacaa gatgatggtc tgccaagtgg gtggtataga ggctcttgtg
     2821 cgtactgtcc ttcgggctgg tgacagggaa gacatcactg agcctgccat ctgtgctctt
     2881 cgtcatctga ccagccgaca ccaagaagca gagatggccc agaatgcagt tcgccttcac
     2941 tatggactac cagttgtggt taagctctta cacccaccat cccactggcc tctgataaag
     3001 gctactgttg gattgattcg aaatcttgcc ctttgtcccg caaatcatgc acctttgcgt
     3061 gagcagggtg ccattccacg actagttcag ttgcttgttc gtgcacatca ggatacccag
     3121 cgccgtacgt ccatgggtgg gacacagcag caatttgtgg agggggtccg catggaagaa
     3181 atagttgaag gttgtaccgg agcccttcac atcctagctc gggatgttca caaccgaatt
     3241 gttatcagag gactaaatac cattccattg tttgtgcagc tgctttattc tcccattgaa
     3301 aacatccaaa gagtagctgc aggggtcctc tgtgaacttg ctcaggacaa ggaagctgca
     3361 gaagctattg aagctgaggg agccacagct cctctgacag agttacttca ctctaggaat
     3421 gaaggtgtgg cgacatatgc agctgctgtt ttgttccgaa tgtctgagga caagccacaa
     3481 gattacaaga aacggctttc agttgagctg accagctctc tcttcagaac agagccaatg
     3541 gcttggaatg agactgctga tcttggactt gatattggtg cccagggaga accccttgga
     3601 tatcgccagg atgatcctag ctatcgttct tttcactctg gtggatatgg ccaggatgcc
     3661 ttgggtatgg accccatgat ggaacatgag atgggtggcc accaccctgg tgctgactat
     3721 ccagttgatg ggctgccaga tctggggcat gcccaggacc tcatggatgg gctgcctcca
     3781 ggtgacagca atcagctggc ctggtttgat actgacctgt aactcgagcg gccgcatcgt
     3841 gactgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     3901 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     3961 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     4021 gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     4081 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4141 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4201 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4261 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4321 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4381 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4441 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     4501 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4561 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4621 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4681 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4741 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     4801 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4861 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4921 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4981 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5041 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5101 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5161 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5221 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5281 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5341 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5401 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5461 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5521 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5581 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5641 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5701 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5761 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5821 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5881 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5941 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6001 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     6061 tattttctcc ttacgcatct gtgcggtatt tcacaccgca taaattccga caccatcgaa
     6121 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg
     6181 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag
     6241 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg
     6301 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc
     6361 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa
     6421 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg
     6481 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc
     6541 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct
     6601 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt
     6661 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt
     6721 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg
     6781 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc
     6841 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt
     6901 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc
     6961 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac
     7021 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa
     7081 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg
     7141 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct
     7201 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     7261 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cccaggct
//
