LOCUS       sequence-259630-       11297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus production plasmid encoding for Archon1 fluorescent 
            voltage reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum
            DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades
            JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C,
            Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, 
            Sabatini BL, Boyden ES
  TITLE     A robotic multidimensional directed evolution approach applied to 
            fluorescent voltage reporters.
  JOURNAL   Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9.
  PUBMED    29483642
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9."
COMMENT     Sequence Label: pHR-EF1a-Archon1-KGC-EGFP-ER2
FEATURES             Location/Qualifiers
     source          1..11297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     178..197
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     437..458
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1364..1373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1417..1436)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1418..1436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1453..2631)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1462..2400
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(1485..1505)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2697..2814
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3099..3143)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3328..3561
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4058..4183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4230..4863
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5031..5165
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5055..5074)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5109..5128
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5590..5610)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5740..5805
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(6939..7268)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6953..7088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7253..7273
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(7343..7360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7514..8102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7594..7613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8273..9064,9065..9133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8896..8915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9134..9238)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9306..9324
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9362..9384)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9484..9503
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9717..9736
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        10026..10044
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10026..10043
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             10069..10302
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     10392..10408
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     10393..10409
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10412..11000
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             10518..10529
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     10927..10947
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(11084..11297,1..497,498..500))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(11084..11297,1..497,498..500))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(11126..11147)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
       61 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      121 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      181 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      241 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      301 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcaa
      361 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      421 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      481 agctcctcgc ccttgctcac cacgttgatg tcgatctggt ccagggggat gtactcgccc
      541 tcgctggtga tcctgctctt ggcggccgcc ttgctcactg ctactaccgg gcggtcggcg
      601 gcaccggtat cagctccagc tgaaggttca ggtgcttctg tatcttgcag aatggctctt
      661 gacctgagca ggataaaacc gaagcccacc ttggcggtga cgtccagcac catgaacagc
      721 aaagtttcga ttcccaggcc cacaactccg gcaccctcgg tgccgataat ccagatgata
      781 gggtaagcag tccacaggac cagaaccagt gcggtgagga tgttgaaggt tgaggcaacc
      841 tctgggcctc tctccttagc ggctgaccgc agagaagtgg ccaggacata cagaacgacg
      901 atcatacaga tagttgagaa cagccaccaa gagtatctgg caatggcagt atgtgacagt
      961 gttcctatca ggcctgtgac aatcatcaga gcatcgacgc caacgagagt gccgatggtc
     1021 actctatcaa ctttggccag gagtgccagg tgcagcagca acagtggtgt agtgaacagc
     1081 cactgtgcgt atcttgcgta gtagatgttc agcatctctc cacctactgg cacctctgtg
     1141 aggccaatac caaagaacat tgacagataa gctgcactgg caataccgct gaccaggatt
     1201 gtcactgcgt agtactcccg tgcatccttg tcagtctctc cccacgctct aaccaggaag
     1261 tagaaggttc cgatcagcat cagcagtgta ccaattccga gccacagact ttcaggtcta
     1321 ccatctccga ggaggtcata accagcctgc agtgcgatag aaaccatggt ggcggatccg
     1381 agctcggtac caagcttacc tagccagctt gggtctccct atagtgagtc gtattagtac
     1441 caagctaatt cctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg
     1501 cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa
     1561 gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata
     1621 aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg
     1681 cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg acggaaaggc
     1741 ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt
     1801 tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga
     1861 ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgaggc caggcaccag
     1921 agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg ctcgcaggcc
     1981 ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg cggccccaaa
     2041 aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt gccagaaaaa
     2101 aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga aagcagcgag
     2161 acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag gccaggcctc
     2221 aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct cccacccact
     2281 tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag taattcaagg
     2341 cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac
     2401 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat
     2461 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca
     2521 gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt
     2581 ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc ctcacggaat
     2641 tcgatatcaa gcttatcgat ccaaactgga tctctgctgt ccctgtaata aacccgaaaa
     2701 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     2761 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
     2821 atgaatactg ccatttggcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct
     2881 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
     2941 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
     3001 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
     3061 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
     3121 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
     3181 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
     3241 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
     3301 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
     3361 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
     3421 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
     3481 ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag
     3541 aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc
     3601 tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat
     3661 tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag cggccggccg
     3721 cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct
     3781 tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata
     3841 atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc
     3901 tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc
     3961 ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt
     4021 tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct
     4081 ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc
     4141 ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt
     4201 tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg
     4261 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     4321 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     4381 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc
     4441 aggatctgag ggctcgccac tccccagtcc cgcccaggcc acgcctccct ggaaagtccc
     4501 cagcggaaag tcccttgtag caagctcgat atcagcagtt cttgaagtac tccggatgca
     4561 gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa cctccactct aacacttctc
     4621 tctccgggtc atccatccca tgcaggctca cagggtgtaa caagctggtg ttctctcctt
     4681 tattggcctc ttctacctta tctggctcaa ctggtactag cttgtagcac catccaaagg
     4741 tcagtggata tctgacccct ggccctggtg tgtagttctg ctaatcaggg aagtagcctt
     4801 gtgtgtggta gatccacaga tcaaggatat cttgtcttct ttgggagtga attagccctt
     4861 ccaactacta agtttgtagt acatatttaa caaatacaat ttctttaaaa tgaaaataat
     4921 tcagaggaat cacaggttta gagtaaatga aaccacaggt aattggcagt ggtaataggg
     4981 tatggggtgg gaagtttggg atgattttgg ttagcttgag ttatccagtt gatccagaca
     5041 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5101 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5161 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5221 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctctagtcaa
     5281 ggcactatac atcaaatatt ccttattaac ccctttacaa attaaaaagc taaaggtaca
     5341 caatttttga gcatagttat taatagcaga cactctatgc ctgtgtggag taagaaaaaa
     5401 cagtatgtta tgattataac tgttatgcct acttataaag gttacagaat atttttccat
     5461 aattttcttg tatagcagtg cagctttttc ctttgtggtg taaatagcaa agcaagcaag
     5521 agttctatta ctaaacacag catgactcaa aaaacttagc aattctgaag gaaagtcctt
     5581 ggggtcttct acctttctct tcttttttgg aggagtagaa tgttgagagt cagcagtagc
     5641 ctcatcatca ctagatggca tttcttctga gcaaaacagg ttttcctcat taaaggcatt
     5701 ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag
     5761 aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat
     5821 ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagat
     5881 ccggggccca ctcataaatc cagttgccgc cacggtagcc aatcaccgta tcgtataaat
     5941 catcgtcggt acgttcggca tcgctcatca caatacgtgc ctggacgtcg aggatttcgc
     6001 gtgggtcaat gccgcgccag atccacatca gacggttaat catgcgatac cagtgaggga
     6061 tggttttacc atcaagggcc gactgcacag gcggttgtgc gccgtgatta aagcggcgga
     6121 ctagcgtcga ggtttcagga tgtttaaagc ggggtttgaa cagggtttcg ctcaggtttg
     6181 cctgtgtcat ggatgcagcc tccagaatac ttactggaaa ctattgtaac ccgcctgaag
     6241 ttaaaaagaa caacgcccgg cagtgccagg cgttgaaaag attagcgacc ggagattggc
     6301 gggacgaata cgacgcccat atcccacggc tgttcaatcc aggtatcttg cgggatatca
     6361 acaacatagt catcaaccag cggacgacca gccggttttg cgaagatggt gacaaagtgc
     6421 gcttttggat acatttcacg aatcgcaacc gcagtaccac cggtatccac caggtcatca
     6481 ataacgatga agccttcgcc atcgccttct gcgcgtttca gcactttaag ctcgcgctgg
     6541 ttgtcgtgat cgtagctgga aatacaaacg gtatcgacat gacgaatacc cagttcacgc
     6601 gccagtaacg cacccggtac agaccgccac ggcttacggc aataatgcct ttccattgtt
     6661 cagaaggcat cagtcggctt gcgagtttac gtgcatggat ctgcaacatg tcccaggtga
     6721 cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt atctacggct taaaaagtgt
     6781 tcgaggggaa aataggttgc gcgagattat agagatctgg cgcactaaaa accagtattt
     6841 cacatgagtc cgcgtctttt tacgcactgc ctctccctga cgcgggataa agtggtattc
     6901 tcaaacatat ctcgcaagcc tgtcttgtgt ccaagctttt tgcaaaagcc taggcctcca
     6961 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     7021 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     7081 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     7141 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     7201 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     7261 tgacacacat tccacagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9241 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9301 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     9361 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9421 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9481 atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc cttatgcgac
     9541 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
     9601 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
     9661 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
     9721 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     9781 cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct gaccatttcc
     9841 gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg
     9901 gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc
     9961 ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
    10021 atacgattta ggtgacacta tagaatacac ggaattaatt ctagatgctg ctagagattt
    10081 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
    10141 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
    10201 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa
    10261 aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta
    10321 aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt accgagctcg
    10381 aattaattcc ctcgaggtcg acggtatcga tgcggggagg cggcccaaag ggagatccga
    10441 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
    10501 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
    10561 atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc
    10621 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
    10681 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
    10741 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
    10801 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
    10861 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
    10921 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
    10981 ttgtaatcca gaggttgatt atcgataagc ttgatatcaa gcttgcatgc ctgcaggtcg
    11041 actctagagt cgcggccgct tacacctcgt tctcgtagca gaacttgtac agctcgtcca
    11101 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
    11161 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
    11221 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
    11281 cctcgatgtt gtggcgg
//
