LOCUS       sequence-259664-       13471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human LRP6  E1E4 (20–1244) in Hi5 insect cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13471)
  AUTHORS   Cheng Z, Biechele T, Wei Z, Morrone S, Moon RT, Wang L, Xu W
  TITLE     Crystal structures of the extracellular domain of LRP6 and its 
            complex with DKK1.
  JOURNAL   Nat Struct Mol Biol. 2011 Oct 9;18(11):1204-10. doi: 
            10.1038/nsmb.2139.
  PUBMED    21984209
REFERENCE   2  (bases 1 to 13471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-10-9- 9"; volume: 
            "18"; issue: "11"; pages: "1204-10"
COMMENT     Sequence Label: pAcGP67A human LRP6 E1E4 (20–1244)
FEATURES             Location/Qualifiers
     source          1..13471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..33
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3709..3732
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_recomb     4231..4936
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           4319..4691
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(4348..4936)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(5370..5387)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6604..6620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6604..6620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6617..6639)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6628..6644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6652..6658,6659..6676,6677..6682))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6697..6718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6835..6852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7006..7594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7086..7105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7765..8556,8557..8625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8388..8407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8626..8730)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8798..8816
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8854..8876)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8976..8995
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9189..9211
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9203..9220
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9204..9220
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     12393..13220
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             12393..12517
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(12555..13160)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        13224..13315
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     13240..13257
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     13412..13471
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
ORIGIN
        1 gcggatccac accatcacca tcaccatcac catgcccctt tgttgcttta tgcaaacaga
       61 cgggacttgc gattggttga tgctacaaat ggcaaagaga atgctacgat tgtagttgga
      121 ggcttggagg atgcagctgc ggtggacttt gtgtttagtc atggcttgat atactggagt
      181 gatgtcagcg aagaagccat taaacgaaca gaatttaaca aaactgagag tgtgcagaat
      241 gttgttgttt ctggattatt gtcccccgat gggctggcat gtgattggct tggagaaaaa
      301 ttgtactgga cagattctga aactaatcgg attgaagttt ctaatttaga tggatcttta
      361 cgaaaagttt tattttggca agagttggat caacccagag ctattgcctt agatccttca
      421 agtgggttca tgtactggac agactgggga gaagtgccaa agatagaacg tgctggaatg
      481 gatggttcaa gtcgcttcat tataataaac agtgaaattt actggccaaa tggactgact
      541 ttggattatg aagaacaaaa gctttattgg gcagatgcaa aacttaattt catccacaaa
      601 tcaaatctgg atggaacaaa tcggcaggca gtggttaaag gttcccttcc acatcctttt
      661 gccttgacgt tatttgagga catattgtac tggactgact ggagcacaca ctccattttg
      721 gcttgcaaca agtatactgg tgagggtctg cgtgaaatcc attctgacat cttctctccc
      781 atggatatac atgccttcag ccaacagagg cagccaaatg ccacaaatcc atgtggaatt
      841 gacaatgggg gttgttccca tttgtgtttg atgtctccag tcaagccttt ttatcagtgt
      901 gcttgcccca ctggggtcaa actcctggag aatggaaaaa cctgcaaaga tggtgccaca
      961 gaattattgc ttttagctcg aaggacagac ttgagacgca tttctttgga tacaccagat
     1021 tttacagaca ttgttctgca gttagaagac atccgtcatg ccattgccat agattacgat
     1081 cctgtggaag gctacatcta ctggactgat gatgaagtga gggccatacg ccgttcattt
     1141 atagatggat ctggcagtca gtttgtggtc actgctcaaa ttgcccatcc tgatggtatt
     1201 gctgtggact gggttgcacg aaatctttat tggacagaca ctggcactga tcgaatagaa
     1261 gtgacaaggc tcaatgggac catgaggaag atcttgattt cagaggactt agaggaaccc
     1321 cgggctattg tgttagatcc catggttggg tacatgtatt ggactgactg gggagaaatt
     1381 ccgaaaattg agcgagcagc tctggatggt tctgaccgtg tagtattggt taacacttct
     1441 cttggttggc caaatggttt agccttggat tatgatgaag gcaaaatata ctggggagat
     1501 gccaaaacag acaagattga ggttatgaat actgatggca ctgggagacg agtactagtg
     1561 gaagacaaaa ttcctcacat atttggattt actttgttgg gtgactatgt ttactggact
     1621 gactggcaga ggcgtagcat tgaaagagtt cataaacgaa gtgcagagag ggaagtgatc
     1681 atagatcagc tgcctgacct catgggccta aaggctacaa atgttcatcg agtgattggt
     1741 tccaacccct gtgctgagga aaacggggga tgtagccatc tctgcctcta tagacctcag
     1801 ggccttcgct gtgcttgccc tattggcttt gaactcatca gtgacatgaa gacctgcatt
     1861 gtcccagagg ctttcctttt gttttcacgg agagcagata tcagacgaat ttctctggaa
     1921 acaaacaata ataatgtggc tattccactc actggtgtca aagaagcttc tgctttggat
     1981 tttgatgtga cagacaaccg aatttattgg actgatatat cactcaagac catcagcaga
     2041 gcctttatga atggcagtgc actggaacat gtggtagaat tcggcttaga ttatccagaa
     2101 ggcatggcag tagactggct tgggaagaac ttgtactggg cagacacagg aacgaatcga
     2161 attgaggtgt caaagttgga tgggcagcac cgacaagttt tggtgtggaa agacctagat
     2221 agtcccagag ctctcgcgtt ggaccctgcc gaaggattta tgtattggac tgaatggggt
     2281 ggaaaaccta agatagacag agctgcaatg gatggaagtg aacgtactac cttagttcca
     2341 aatgtggggc gggcaaacgg cctaactatt gattatgcta aaaggaggct ttattggaca
     2401 gacctggaca ccaacttaat agaatcttca aatatgcttg ggctcaaccg tgaagttata
     2461 gcagatgact tgcctcatcc ttttggctta actcagtacc aagattatat ctactggacg
     2521 gactggagcc gacgcagcat tgagcgtgcc aacaaaacca gtggccaaaa ccgcaccatc
     2581 attcagggcc atttggatta tgtgatggac atcctcgtct ttcactcatc tcgacagtca
     2641 gggtggaatg aatgtgcttc cagcaatggg cactgctccc acctctgctt ggctgtgcca
     2701 gttgggggtt ttgtttgtgg atgccctgcc cactactctc ttaatgctga caacaggact
     2761 tgtagtgctc ctacgacttt cctgctcttc agtcaaaaga gtgccatcaa ccgcatggtg
     2821 attgatgaac aacagagccc cgacatcatc cttcccatcc acagccttcg gaatgtccgg
     2881 gccattgact atgacccact ggacaagcaa ctctattgga ttgactcacg acaaaacatg
     2941 atccgaaagg cacaagaaga tggcagccag ggctttactg tggttgtgag ctcagttccg
     3001 agtcagaacc tggaaataca accctatgac ctcagcattg atatttacag ccgctacatc
     3061 tactggactt gtgaggctac caatgtcatt aatgtgacaa gattagatgg gagatcagtt
     3121 ggagtggtgc tgaaaggcga gcaggacaga cctcgagcca ttgtggtaaa cccagagaaa
     3181 gggtatatgt attttaccaa tcttcaggaa aggtctccta aaattgaacg ggctgctttg
     3241 gatgggacag aacgggaggt cctctttttc agtggcttaa gtaaaccaat tgctttagcc
     3301 cttgatagca ggctgggcaa gctcttttgg gctgattcag atctccggcg aattgaaagc
     3361 agtgatctct caggtgctaa ccggatagta ttagaagact ccaatatctt gcagcctgtg
     3421 ggacttactg tgtttgaaaa ctggctctat tggattgata aacagcagca aatgattgaa
     3481 aaaattgaca tgacaggtcg agagggtaga accaaagtcc aagctcgaat tgcccagctt
     3541 agtgacattc atgcagtaaa ggagctgaac cttcaagaat acagacagca cccttgtgct
     3601 caggataatg gtggctgttc acatatttgt cttgtaaagg gggatggtac tacaaggtgt
     3661 tcttgcccca tgcacctggt tctacttcaa gatgagctat catgtggatg gagccacccg
     3721 cagttcgaaa agtagcggcc gctgcagatc tgatcctttc ctgggacccg gcaagaacca
     3781 aaaactcact ctcttcaagg aaatccgtaa tgttaaaccc gacacgatga agcttgtcgt
     3841 tggatggaaa ggaaaagagt tctacaggga aacttggacc cgcttcatgg aagacagctt
     3901 ccccattgtt aacgaccaag aagtgatgga tgttttcctt gttgtcaaca tgcgtcccac
     3961 tagacccaac cgttgttaca aattcctggc ccaacacgct ctgcgttgcg accccgacta
     4021 tgtacctcat gacgtgatta ggatcgtcga gccttcatgg gtgggcagca acaacgagta
     4081 ccgcatcagc ctggctaaga agggcggcgg ctgcccaata atgaaccttc actctgagta
     4141 caccaactcg ttcgaacagt tcatcgatcg tgtcatctgg gagaacttct acaagcccat
     4201 cgtttacatc ggtaccgact ctgctgaaga ggaggaaatt ctccttgaag tttccctggt
     4261 gttcaaagta aaggagtttg caccagacgc acctctgttc actggtccgg cgtattaaaa
     4321 cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
     4381 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
     4441 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
     4501 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
     4561 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
     4621 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
     4681 caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga
     4741 cgtaaacacg ttaaataaag cttggacata tttaacatcg ggcgtgttag ctttattagg
     4801 ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc
     4861 catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt
     4921 tgagcttttt ggaattattt ctgattgcgg gcgtttttgg gcgggtttca atctaactgt
     4981 gcccgatttt aattcagaca acacgttaga aagcgatggt gcaggcggtg gtaacatttc
     5041 agacggcaaa tctactaatg gcggcggtgg tggagctgat gataaatcta ccatcggtgg
     5101 aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg
     5161 cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc acctcaacta ttgtactggt
     5221 ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg cgattttttt cgtttctaat
     5281 agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat
     5341 tggtggagcg ggcggcaatt cagacatcga tggtggtggt ggtggtggag gcgctggaat
     5401 gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt ctggtttagt
     5461 ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg
     5521 tctgcttcga ggcagcgctt ggggtggtgg caattcaata ttataattgg aatacaaatc
     5581 gtaaaaatct gctataagca ttgtaatttc gctatcgttt accgtgccga tatttaacaa
     5641 ccgctcaatg taagcaattg tattgtaaag agattgtctc aagctcgcac gccgataaca
     5701 agccttttca tttttactac agcattgtag tggcgagaca cttcgctgtc gtcgacgtac
     5761 atgtatgctt tgttgtcaaa aacgtcgttg gcaagcttta aaatatttaa aagaacatct
     5821 ctgttcagca ccactgtgtt gtcgtaaatg ttgtttttga taatttgcgc ttccgcagta
     5881 tcgacacgtt caaaaaattg atgcgcatca attttgttgt tcctattatt gaataaataa
     5941 gattgtacag attcatatct acgattcgtc atggccacca caaatgctac gctgcaaacg
     6001 ctggtacaat tttacgaaaa ctgcaaaaac gtcaaaactc ggtataaaat aatcaacggg
     6061 cgctttggca aaatatctat tttatcgcac aagcccacta gcaaattgta tttgcagaaa
     6121 acaatttcgg cgcacaattt taacgctgac gaaataaaag ttcaccagtt aatgagcgac
     6181 cacccaaatt ttataaaaat ctattttaat cacggttcca tcaacaacca agtgatcgtg
     6241 atggactaca ttgactgtcc cgatttattt gaaacactac aaattaaagg cgagctttcg
     6301 taccaacttg ttagcaatat tattagacag ctgtgtgaag cgctcaacga tttgcacaag
     6361 cacaatttca tacacaacga cataaaactc gaaaatgtct tatatttcga agcacttgat
     6421 cgcgtgtatg tttgcgatta cggattgtgc aaacacgaaa actcacttag cgtgcacgac
     6481 ggcacgttgg agtattttag tccggaaaaa attcgacacc acaactatgc acgttcgttt
     6541 gactggtacg ccgtcggcgt gttaacatac aagttgctaa ccggcggcca attcgtaatc
     6601 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6661 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6721 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8761 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     8821 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     8881 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     8941 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     9001 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     9061 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     9121 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     9181 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg gctcgtaaag
     9241 cgagttgaag gatcatattt agttgcgttt atgagataag attgaaagca cgtgtaaaat
     9301 gtttcccgcg cgttggcaca actatttaca atgcggccaa gttataaaag attctaatct
     9361 gatatgtttt aaaacacctt tgcggcccga gttgtttgcg tacgtgacta gcgaagaaga
     9421 tatgtggacc gcagaacaga tagtaaaaca aaaccctagt attggagcaa taatcgattt
     9481 aaccaacacg tctaaatatt atgatggtgt gcattttttg cgggcgggcc tgttatacaa
     9541 aaaaattcaa gtacctggcc agactttgcc gcctgaaagc atagttcaag aatttattga
     9601 cacggtaaaa gaatttacag aaaagtgtcc cggcatgttg gtgggcgtgc actgcacaca
     9661 cggtattaat cgcaccggtt acatggtgtg cagatattta atgcacaccc tgggtattgc
     9721 gccgcaggaa gccatagata gattcgaaaa agccagaggt cacaaaattg aaagacaaaa
     9781 ttacgttcaa gatttattaa tttaattaat attatttgca ttctttaaca aatactttat
     9841 cctattttca aattgttgcg cttcttccag cgaaccaaaa ctatgcttcg cttgctccgt
     9901 ttagcttgta gccgatcagt ggcgttgttc caatcgacgg taggattagg ccggatattc
     9961 tccaccacaa tgttggcaac gttgatgtta cgtttatgct tttggttttc cacgtacgtc
    10021 ttttggccgg taatagccgt aaacgtagtg ccgtcgcgcg tcacgcacaa caccggatgt
    10081 ttgcgcttgt ccgcggggta ttgaaccgcg cgatccgaca aatccaccac tttggcaact
    10141 aaatcggtga cctgcgcgtc ttttttctgc attatttcgt ctttcttttg catggtttcc
    10201 tggaagccgg tgtacatgcg gtttagatca gtcatgacgc gcgtgacctg caaatctttg
    10261 gcctcgatct gcttgtcctt gatggcaacg atgcgttcaa taaactcttg ttttttaaca
    10321 agttcctcgg ttttttgcgc caccaccgct tgcagcgcgt ttgtgtgctc ggtgaatgtc
    10381 gcaatcagct tagtcaccaa ctgtttgctc tcctcctccc gttgtttgat cgcgggatcg
    10441 tacttgccgg tgcagagcac ttgaggaatt acttcttcta aaagccattc ttgtaattct
    10501 atggcgtaag gcaatttgga cttcataatc agctgaatca cgccggattt agtaatgagc
    10561 actgtatgcg gctgcaaata cagcgggtcg ccccttttca cgacgctgtt agaggtaggg
    10621 cccccatttt ggatggtctg ctcaaataac gatttgtatt tattgtctac atgaacacgt
    10681 atagctttat cacaaactgt atattttaaa ctgttagcga cgtccttggc cacgaaccgg
    10741 acctgttggt cgcgctctag cacgtaccgc aggttgaacg tatcttctcc aaatttaaat
    10801 tctccaattt taacgcgagc cattttgata cacgtgtgtc gattttgcaa caactattgt
    10861 tttttaacgc aaactaaact tattgtggta agcaataatt aaatatgggg gaacatgcgc
    10921 cgctacaaca ctcgtcgtta tgaacgcaga cggcgccggt ctcggcgcaa gcggctaaaa
    10981 cgtgttgcgc gttcaacgcg gcaaacatcg caaaagccaa tagtacagtt ttgatttgca
    11041 tattaacggc gattttttaa attatcttat ttaataaata gttatgacgc ctacaactcc
    11101 ccgcccgcgt tgactcgctg cacctcgagc agttcgttga cgccttcctc cgtgtggccg
    11161 aacacgtcga gcgggtggtc gatgaccagc ggcgtgccgc acgcgacgca caagtatctg
    11221 tacaccgaat gatcgtcggg cgaaggcacg tcggcctcca agtggcaata ttggcaaatt
    11281 cgaaaatata tacagttggg ttgtttgcgc atatctatcg tggcgttggg catgtacgtc
    11341 cgaacgttga tttgcatgca agccgaaatt aaatcattgc gattagtgcg attaaaacgt
    11401 tgtacatcct cgcttttaat catgccgtcg attaaatcgc gcaatcgagt caagtgatca
    11461 aagtgtggaa taatgttttc tttgtattcc cgagtcaagc gcagcgcgta ttttaacaaa
    11521 ctagccatct tgtaagttag tttcatttaa tgcaacttta tccaataata tattatgtat
    11581 cgcacgtcaa gaattaacaa tgcgcccgtt gtcgcatctc aacacgacta tgatagagat
    11641 caaataaagc gcgaattaaa tagcttgcga cgcaacgtgc acgatctgtg cacgcgttcc
    11701 ggcacgagct ttgattgtaa taagttttta cgaagcgatg acatgacccc cgtagtgaca
    11761 acgatcacgc ccaaaagaac tgccgactac aaaattaccg agtatgtcgg tgacgttaaa
    11821 actattaagc catccaatcg accgttagtc gaatcaggac cgctggtgcg agaagccgcg
    11881 aagtatggcg aatgcatcgt ataacgtgtg gagtccgctc attagagcgt catgtttaga
    11941 caagaaagct acatatttaa ttgatcccga tgattttatt gataaattga ccctaactcc
    12001 atacacggta ttctacaatg gcggggtttt ggtcaaaatt tccggactgc gattgtacat
    12061 gctgttaacg gctccgccca ctattaatga aattaaaaat tccaatttta aaaaacgcag
    12121 caagagaaac atttgtatga aagaatgcgt agaaggaaag aaaaatgtcg tcgacatgct
    12181 gaacaacaag attaatatgc ctccgtgtat aaaaaaaata ttgaacgatt tgaaagaaaa
    12241 caatgtaccg cgcggcggta tgtacaggaa gaggtttata ctaaactgtt acattgcaaa
    12301 cgtggtttcg tgtgccaagt gtgaaaaccg atgtttaatc aaggctctga cgcatttcta
    12361 caaccacgac tccaagtgtg tgggtgaagt catgcatctt ttaatcaaat cccaagatgt
    12421 gtataaacca ccaaactgcc aaaaaatgaa aactgtcgac aagctctgtc cgtttgctgg
    12481 caactgcaag ggtctcaatc ctatttgtaa ttattgaata ataaaacaat tataaatgtc
    12541 aaatttgttt tttattaacg atacaaacca aacgcaacaa gaacatttgt agtattatct
    12601 ataattgaaa acgcgtagtt ataatcgctg aggtaatatt taaaatcatt ttcaaatgat
    12661 tcacagttaa tttgcgacaa tataatttta ttttcacata aactagacgc cttgtcgtct
    12721 tcttcttcgt attccttctc tttttcattt ttctcctcat aaaaattaac atagttatta
    12781 tcgtatccat atatgtatct atcgtataga gtaaattttt tgttgtcata aatatatatg
    12841 tcttttttaa tggggtgtat agtaccgctg cgcatagttt ttctgtaatt tacaacagtg
    12901 ctattttctg gtagttcttc ggagtgtgtt gctttaatta ttaaatttat ataatcaatg
    12961 aatttgggat cgtcggtttt gtacaatatg ttgccggcat agtacgcagc ttcttctagt
    13021 tcaattacac cattttttag cagcaccgga ttaacataac tttccaaaat gttgtacgaa
    13081 ccgttaaaca aaaacagttc acctcccttt tctatactat tgtctgcgag cagttgtttg
    13141 ttgttaaaaa taacagccat tgtaatgaga cgcacaaact aatatcacaa actggaaatg
    13201 tctatcaata tatagttgct gatatcatgg agataattaa aatgataacc atctcgcaaa
    13261 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc
    13321 ggattattca taccgtccca ccatcgggcg cggatctatg ctactagtaa atcagtcaca
    13381 ccaaggcttc aataaggaac acacaagcaa gatggtaagc gctattgttt tatatgtgct
    13441 tttggcggcg gcggcgcatt ctgcctttgc g
//
