LOCUS       sequence-259665-       11617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human LRP6 E3E4 (630–1244) in Hi5 insect cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Cheng Z, Biechele T, Wei Z, Morrone S, Moon RT, Wang L, Xu W
  TITLE     Crystal structures of the extracellular domain of LRP6 and its 
            complex with DKK1.
  JOURNAL   Nat Struct Mol Biol. 2011 Oct 9;18(11):1204-10. doi: 
            10.1038/nsmb.2139.
  PUBMED    21984209
REFERENCE   2  (bases 1 to 11617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-10-9- 9"; volume: 
            "18"; issue: "11"; pages: "1204-10"
COMMENT     Sequence Label: pAcGP67A human LRP6 E3E4 (630–1244)
FEATURES             Location/Qualifiers
     source          1..11617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     1..60
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             70..93
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_recomb     2437..3142
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           2525..2897
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(2554..3142)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(3576..3593)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4810..4826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4810..4826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4823..4845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4834..4850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4858..4864,4865..4882,4883..4888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4903..4924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5041..5058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5212..5800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5292..5311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5971..6762,6763..6831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6594..6613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6832..6936)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7004..7022
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7060..7082)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7182..7201
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7395..7417
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7409..7426
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7410..7426
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     10599..11426
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             10599..10723
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(10761..11366)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        11430..11521
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     11446..11463
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 atggtaagcg ctattgtttt atatgtgctt ttggcggcgg cggcgcattc tgcctttgcg
       61 gcggatccac accatcacca tcaccatcac catccagagg ctttcctttt gttttcacgg
      121 agagcagata tcagacgaat ttctctggaa acaaacaata ataatgtggc tattccactc
      181 actggtgtca aagaagcttc tgctttggat tttgatgtga cagacaaccg aatttattgg
      241 actgatatat cactcaagac catcagcaga gcctttatga atggcagtgc actggaacat
      301 gtggtagaat tcggcttaga ttatccagaa ggcatggcag tagactggct tgggaagaac
      361 ttgtactggg cagacacagg aacgaatcga attgaggtgt caaagttgga tgggcagcac
      421 cgacaagttt tggtgtggaa agacctagat agtcccagag ctctcgcgtt ggaccctgcc
      481 gaaggattta tgtattggac tgaatggggt ggaaaaccta agatagacag agctgcaatg
      541 gatggaagtg aacgtactac cttagttcca aatgtggggc gggcaaacgg cctaactatt
      601 gattatgcta aaaggaggct ttattggaca gacctggaca ccaacttaat agaatcttca
      661 aatatgcttg ggctcaaccg tgaagttata gcagatgact tgcctcatcc ttttggctta
      721 actcagtacc aagattatat ctactggacg gactggagcc gacgcagcat tgagcgtgcc
      781 aacaaaacca gtggccaaaa ccgcaccatc attcagggcc atttggatta tgtgatggac
      841 atcctcgtct ttcactcatc tcgacagtca gggtggaatg aatgtgcttc cagcaatggg
      901 cactgctccc acctctgctt ggctgtgcca gttgggggtt ttgtttgtgg atgccctgcc
      961 cactactctc ttaatgctga caacaggact tgtagtgctc ctacgacttt cctgctcttc
     1021 agtcaaaaga gtgccatcaa ccgcatggtg attgatgaac aacagagccc cgacatcatc
     1081 cttcccatcc acagccttcg gaatgtccgg gccattgact atgacccact ggacaagcaa
     1141 ctctattgga ttgactcacg acaaaacatg atccgaaagg cacaagaaga tggcagccag
     1201 ggctttactg tggttgtgag ctcagttccg agtcagaacc tggaaataca accctatgac
     1261 ctcagcattg atatttacag ccgctacatc tactggactt gtgaggctac caatgtcatt
     1321 aatgtgacaa gattagatgg gagatcagtt ggagtggtgc tgaaaggcga gcaggacaga
     1381 cctcgagcca ttgtggtaaa cccagagaaa gggtatatgt attttaccaa tcttcaggaa
     1441 aggtctccta aaattgaacg ggctgctttg gatgggacag aacgggaggt cctctttttc
     1501 agtggcttaa gtaaaccaat tgctttagcc cttgatagca ggctgggcaa gctcttttgg
     1561 gctgattcag atctccggcg aattgaaagc agtgatctct caggtgctaa ccggatagta
     1621 ttagaagact ccaatatctt gcagcctgtg ggacttactg tgtttgaaaa ctggctctat
     1681 tggattgata aacagcagca aatgattgaa aaaattgaca tgacaggtcg agagggtaga
     1741 accaaagtcc aagctcgaat tgcccagctt agtgacattc atgcagtaaa ggagctgaac
     1801 cttcaagaat acagacagca cccttgtgct caggataatg gtggctgttc acatatttgt
     1861 cttgtaaagg gggatggtac tacaaggtgt tcttgcccca tgcacctggt tctacttcaa
     1921 gatgagctat catgtggata gcggccgctg cagatctgat cctttcctgg gacccggcaa
     1981 gaaccaaaaa ctcactctct tcaaggaaat ccgtaatgtt aaacccgaca cgatgaagct
     2041 tgtcgttgga tggaaaggaa aagagttcta cagggaaact tggacccgct tcatggaaga
     2101 cagcttcccc attgttaacg accaagaagt gatggatgtt ttccttgttg tcaacatgcg
     2161 tcccactaga cccaaccgtt gttacaaatt cctggcccaa cacgctctgc gttgcgaccc
     2221 cgactatgta cctcatgacg tgattaggat cgtcgagcct tcatgggtgg gcagcaacaa
     2281 cgagtaccgc atcagcctgg ctaagaaggg cggcggctgc ccaataatga accttcactc
     2341 tgagtacacc aactcgttcg aacagttcat cgatcgtgtc atctgggaga acttctacaa
     2401 gcccatcgtt tacatcggta ccgactctgc tgaagaggag gaaattctcc ttgaagtttc
     2461 cctggtgttc aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta
     2521 ttaaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg
     2581 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
     2641 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
     2701 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
     2761 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
     2821 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
     2881 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
     2941 actcgacgta aacacgttaa ataaagcttg gacatattta acatcgggcg tgttagcttt
     3001 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
     3061 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
     3121 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
     3181 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
     3241 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
     3301 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
     3361 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
     3421 actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt
     3481 tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt
     3541 cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc
     3601 tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg
     3661 tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga
     3721 aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata
     3781 caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt
     3841 taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tcgcacgccg
     3901 ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc gctgtcgtcg
     3961 acgtacatgt atgctttgtt gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga
     4021 acatctctgt tcagcaccac tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc
     4081 gcagtatcga cacgttcaaa aaattgatgc gcatcaattt tgttgttcct attattgaat
     4141 aaataagatt gtacagattc atatctacga ttcgtcatgg ccaccacaaa tgctacgctg
     4201 caaacgctgg tacaatttta cgaaaactgc aaaaacgtca aaactcggta taaaataatc
     4261 aacgggcgct ttggcaaaat atctatttta tcgcacaagc ccactagcaa attgtatttg
     4321 cagaaaacaa tttcggcgca caattttaac gctgacgaaa taaaagttca ccagttaatg
     4381 agcgaccacc caaattttat aaaaatctat tttaatcacg gttccatcaa caaccaagtg
     4441 atcgtgatgg actacattga ctgtcccgat ttatttgaaa cactacaaat taaaggcgag
     4501 ctttcgtacc aacttgttag caatattatt agacagctgt gtgaagcgct caacgatttg
     4561 cacaagcaca atttcataca caacgacata aaactcgaaa atgtcttata tttcgaagca
     4621 cttgatcgcg tgtatgtttg cgattacgga ttgtgcaaac acgaaaactc acttagcgtg
     4681 cacgacggca cgttggagta ttttagtccg gaaaaaattc gacaccacaa ctatgcacgt
     4741 tcgtttgact ggtacgccgt cggcgtgtta acatacaagt tgctaaccgg cggccaattc
     4801 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4861 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4921 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5641 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6961 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7021 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     7081 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     7141 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     7201 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7261 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     7321 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     7381 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttggctc
     7441 gtaaagcgag ttgaaggatc atatttagtt gcgtttatga gataagattg aaagcacgtg
     7501 taaaatgttt cccgcgcgtt ggcacaacta tttacaatgc ggccaagtta taaaagattc
     7561 taatctgata tgttttaaaa cacctttgcg gcccgagttg tttgcgtacg tgactagcga
     7621 agaagatatg tggaccgcag aacagatagt aaaacaaaac cctagtattg gagcaataat
     7681 cgatttaacc aacacgtcta aatattatga tggtgtgcat tttttgcggg cgggcctgtt
     7741 atacaaaaaa attcaagtac ctggccagac tttgccgcct gaaagcatag ttcaagaatt
     7801 tattgacacg gtaaaagaat ttacagaaaa gtgtcccggc atgttggtgg gcgtgcactg
     7861 cacacacggt attaatcgca ccggttacat ggtgtgcaga tatttaatgc acaccctggg
     7921 tattgcgccg caggaagcca tagatagatt cgaaaaagcc agaggtcaca aaattgaaag
     7981 acaaaattac gttcaagatt tattaattta attaatatta tttgcattct ttaacaaata
     8041 ctttatccta ttttcaaatt gttgcgcttc ttccagcgaa ccaaaactat gcttcgcttg
     8101 ctccgtttag cttgtagccg atcagtggcg ttgttccaat cgacggtagg attaggccgg
     8161 atattctcca ccacaatgtt ggcaacgttg atgttacgtt tatgcttttg gttttccacg
     8221 tacgtctttt ggccggtaat agccgtaaac gtagtgccgt cgcgcgtcac gcacaacacc
     8281 ggatgtttgc gcttgtccgc ggggtattga accgcgcgat ccgacaaatc caccactttg
     8341 gcaactaaat cggtgacctg cgcgtctttt ttctgcatta tttcgtcttt cttttgcatg
     8401 gtttcctgga agccggtgta catgcggttt agatcagtca tgacgcgcgt gacctgcaaa
     8461 tctttggcct cgatctgctt gtccttgatg gcaacgatgc gttcaataaa ctcttgtttt
     8521 ttaacaagtt cctcggtttt ttgcgccacc accgcttgca gcgcgtttgt gtgctcggtg
     8581 aatgtcgcaa tcagcttagt caccaactgt ttgctctcct cctcccgttg tttgatcgcg
     8641 ggatcgtact tgccggtgca gagcacttga ggaattactt cttctaaaag ccattcttgt
     8701 aattctatgg cgtaaggcaa tttggacttc ataatcagct gaatcacgcc ggatttagta
     8761 atgagcactg tatgcggctg caaatacagc gggtcgcccc ttttcacgac gctgttagag
     8821 gtagggcccc cattttggat ggtctgctca aataacgatt tgtatttatt gtctacatga
     8881 acacgtatag ctttatcaca aactgtatat tttaaactgt tagcgacgtc cttggccacg
     8941 aaccggacct gttggtcgcg ctctagcacg taccgcaggt tgaacgtatc ttctccaaat
     9001 ttaaattctc caattttaac gcgagccatt ttgatacacg tgtgtcgatt ttgcaacaac
     9061 tattgttttt taacgcaaac taaacttatt gtggtaagca ataattaaat atgggggaac
     9121 atgcgccgct acaacactcg tcgttatgaa cgcagacggc gccggtctcg gcgcaagcgg
     9181 ctaaaacgtg ttgcgcgttc aacgcggcaa acatcgcaaa agccaatagt acagttttga
     9241 tttgcatatt aacggcgatt ttttaaatta tcttatttaa taaatagtta tgacgcctac
     9301 aactccccgc ccgcgttgac tcgctgcacc tcgagcagtt cgttgacgcc ttcctccgtg
     9361 tggccgaaca cgtcgagcgg gtggtcgatg accagcggcg tgccgcacgc gacgcacaag
     9421 tatctgtaca ccgaatgatc gtcgggcgaa ggcacgtcgg cctccaagtg gcaatattgg
     9481 caaattcgaa aatatataca gttgggttgt ttgcgcatat ctatcgtggc gttgggcatg
     9541 tacgtccgaa cgttgatttg catgcaagcc gaaattaaat cattgcgatt agtgcgatta
     9601 aaacgttgta catcctcgct tttaatcatg ccgtcgatta aatcgcgcaa tcgagtcaag
     9661 tgatcaaagt gtggaataat gttttctttg tattcccgag tcaagcgcag cgcgtatttt
     9721 aacaaactag ccatcttgta agttagtttc atttaatgca actttatcca ataatatatt
     9781 atgtatcgca cgtcaagaat taacaatgcg cccgttgtcg catctcaaca cgactatgat
     9841 agagatcaaa taaagcgcga attaaatagc ttgcgacgca acgtgcacga tctgtgcacg
     9901 cgttccggca cgagctttga ttgtaataag tttttacgaa gcgatgacat gacccccgta
     9961 gtgacaacga tcacgcccaa aagaactgcc gactacaaaa ttaccgagta tgtcggtgac
    10021 gttaaaacta ttaagccatc caatcgaccg ttagtcgaat caggaccgct ggtgcgagaa
    10081 gccgcgaagt atggcgaatg catcgtataa cgtgtggagt ccgctcatta gagcgtcatg
    10141 tttagacaag aaagctacat atttaattga tcccgatgat tttattgata aattgaccct
    10201 aactccatac acggtattct acaatggcgg ggttttggtc aaaatttccg gactgcgatt
    10261 gtacatgctg ttaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa
    10321 acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga
    10381 catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa
    10441 agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat
    10501 tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca
    10561 tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca
    10621 agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt
    10681 tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata
    10741 aatgtcaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta
    10801 ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca
    10861 aatgattcac agttaatttg cgacaatata attttatttt cacataaact agacgccttg
    10921 tcgtcttctt cttcgtattc cttctctttt tcatttttct cctcataaaa attaacatag
    10981 ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata
    11041 tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca
    11101 acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa
    11161 tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct
    11221 tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg
    11281 tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt
    11341 tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata tcacaaactg
    11401 gaaatgtcta tcaatatata gttgctgata tcatggagat aattaaaatg ataaccatct
    11461 cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa aacctataaa
    11521 tattccggat tattcatacc gtcccaccat cgggcgcgga tctatgctac tagtaaatca
    11581 gtcacaccaa ggcttcaata aggaacacac aagcaag
//
