LOCUS       sequence-259670-        9528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to 
            selectively edit adenosine to inosine in RNA when used in conjuction
            with a guide RNA. Cas13b is truncated to allow for expression by 
            AAV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung 
            J, Zhang F
  TITLE     RNA editing with CRISPR-Cas13.
  JOURNAL   Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
  PUBMED    29070703
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180."
COMMENT     Sequence Label: 
            pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1241..1267
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(2476..2493)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2482..2706
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2752..3180
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2839..2858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3049..3070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3189..3209)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3194..3523
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3374..3509
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3436..3455
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3590..4384
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3644..3663)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4254..4273
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4558..4679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4595..4614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4649..4668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4728..4744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4728..4744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4741..4763)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4752..4768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4776..4782,4783..4800,4801..4806))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4821..4842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4959..4976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5130..5718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5210..5229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5889..6680,6681..6749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6512..6531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6750..6854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6929..6948)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7120..7499
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7500..7703
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7654..7674
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7748..7767
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7748..7766
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 attcaaggac gaccggaagt ccattcggag cgccgacaac aagatgggaa agagaggctt
       61 caagcagatc tccacaggca agctggccga cttcctggcc aaggacatcg tgctgtttca
      121 gcccagcgtg aacgatggcg agaacaagat caccggcctg aactaccgga tcatgcagag
      181 cgccattgcc gtgtacgata gcggcgacga ttacgaggcc aagcagcagt tcaagctgat
      241 gttcgagaag gcccggctga tcggcaaggg cacaacagag cctcatccat ttctgtacaa
      301 ggtgttcgcc cgcagcatcc ccgccaatgc cgtcgagttc tacgagcgct acctgatcga
      361 gcggaagttc tacctgaccg gcctgtccaa cgagatcaag aaaggcaaca gagtggatgt
      421 gcccttcatc cggcgggacc agaacaagtg gaaaacaccc gccatgaaga ccctgggcag
      481 aatctacagc gaggatctgc ccgtggaact gcccagacag atgttcgaca atgagatcaa
      541 gtcccacctg aagtccctgc cacagatgga aggcatcgac ttcaacaatg ccaacgtgac
      601 ctatctgatc gccgagtaca tgaagagagt gctggacgac gacttccaga ccttctacca
      661 gtggaaccgc aactaccggt acatggacat gcttaagggc gagtacgaca gaaagggctc
      721 cctgcagcac tgcttcacca gcgtggaaga gagagaaggc ctctggaaag agcgggcctc
      781 cagaacagag cggtacagaa agcaggccag caacaagatc cgcagcaacc ggcagatgag
      841 aaacgccagc agcgaagaga tcgagacaat cctggataag cggctgagca acagccggaa
      901 cgagtaccag aaaagcgaga aagtgatccg gcgctacaga gtgcaggatg ccctgctgtt
      961 tctgctggcc aaaaagaccc tgaccgaact ggccgatttc gacggcgaga ggttcaaact
     1021 gaaagaaatc atgcccgacg ccgagaaggg aatcctgagc gagatcatgc ccatgagctt
     1081 caccttcgag aaaggcggca agaagtacac catcaccagc gagggcatga agctgaagaa
     1141 ctacggcgac ttctttgtgc tggctagcga caagaggatc ggcaacctgc tggaactcgt
     1201 gggcagcgac atcgtgtcca aagaggatgg atcccttcaa ctgcctccac ttgaaagact
     1261 gacactggga tcccagctgc atttaccgca ggttttagct gacgctgtct cacgcctggt
     1321 cctgggtaag tttggtgacc tgaccgacaa cttctcctcc cctcacgctc gcagaaaagt
     1381 gctggctgga gtcgtcatga caacaggcac agatgttaaa gatgccaagg tgataagtgt
     1441 ttctacagga ggcaaatgta ttaatggtga atacatgagt gatcgtggcc ttgcattaaa
     1501 tgactgccat gcagaaataa tatctcggag atccttgctc agatttcttt atacacaact
     1561 tgagctttac ttaaataaca aagatgatca aaaaagatcc atctttcaga aatcagagcg
     1621 aggggggttt aggctgaagg agaatgtcca gtttcatctg tacatcagca cctctccctg
     1681 tggagatgcc agaatcttct caccacatga gccaatcctg gaagaaccag cagatagaca
     1741 cccaaatcgt aaagcaagag gacagctacg gaccaaaata gagtctggtc aggggacgat
     1801 tccagtgcgc tccaatgcga gcatccaaac gtgggacggg gtgctgcaag gggagcggct
     1861 gctcaccatg tcctgcagtg acaagattgc acgctggaac gtggtgggca tccagggatc
     1921 actgctcagc attttcgtgg agcccattta cttctcgagc atcatcctgg gcagccttta
     1981 ccacggggac cacctttcca gggccatgta ccagcggatc tccaacatag aggacctgcc
     2041 acctctctac accctcaaca agcctttgct cagtggcatc agcaatgcag aagcacggca
     2101 gccagggaag gcccccaact tcagtgtcaa ctggacggta ggcgactccg ctattgaggt
     2161 catcaacgcc acgactggga aggatgagct gggccgcgcg tcccgcctgt gtaagcacgc
     2221 gttgtactgt cgctggatgc gtgtgcacgg caaggttccc tcccacttac tacgctccaa
     2281 gattaccaag cccaacgtgt accatgagtc caagctggcg gcaaaggagt accaggccgc
     2341 caaggcgcgt ctgttcacag ccttcatcaa ggcggggctg ggggcctggg tggagaagcc
     2401 caccgagcag gaccagttct cactcacgta agcggccgct cgagtctaga gggcccgttt
     2461 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     2521 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     2581 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     2641 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     2701 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     2761 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2821 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2881 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2941 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3001 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3061 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     3121 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3181 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3241 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3301 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3361 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3421 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     3481 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     3541 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     3601 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     3661 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     3721 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     3781 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     3841 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     3901 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     3961 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4021 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     4081 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     4141 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4201 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4261 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4321 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     4381 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     4441 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4501 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4561 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4621 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4681 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     4741 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4801 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4861 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4921 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4981 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5041 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5101 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5161 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5221 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5281 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5341 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5401 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5461 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5521 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5581 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5641 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5701 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5761 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5821 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5881 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5941 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6001 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6061 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     6901 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6961 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     7021 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     7081 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     7141 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     7201 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     7261 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     7321 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     7381 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     7441 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     7501 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     7561 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     7621 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     7681 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     7741 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct
     7801 tgccaccatg aacatccccg ctctggtgga aaaccagaag aagtactttg gcacctacag
     7861 cgtgatggcc atgctgaacg ctcagaccgt gctggaccac atccagaagg tggccgatat
     7921 tgagggcgag cagaacgaga acaacgagaa tctgtggttt caccccgtga tgagccacct
     7981 gtacaacgcc aagaacggct acgacaagca gcccgagaaa accatgttca tcatcgagcg
     8041 gctgcagagc tacttcccat tcctgaagat catggccgag aaccagagag agtacagcaa
     8101 cggcaagtac aagcagaacc gcgtggaagt gaacagcaac gacatcttcg aggtgctgaa
     8161 gcgcgccttc ggcgtgctga agatgtacag ggacctgacc aacgcataca agacctacga
     8221 ggaaaagctg aacgacggct gcgagttcct gaccagcaca gagcaacctc tgagcggcat
     8281 gatcaacaac tactacacag tggccctgcg gaacatgaac gagagatacg gctacaagac
     8341 agaggacctg gccttcatcc aggacaagcg gttcaagttc gtgaaggacg cctacggcaa
     8401 gaaaaagtcc caagtgaata ccggattctt cctgagcctg caggactaca acggcgacac
     8461 acagaagaag ctgcacctga gcggagtggg aatcgccctg ctgatctgcc tgttcctgga
     8521 caagcagtac atcaacatct ttctgagcag gctgcccatc ttctccagct acaatgccca
     8581 gagcgaggaa cggcggatca tcatcagatc cttcggcatc aacagcatca agctgcccaa
     8641 ggaccggatc cacagcgaga agtccaacaa gagcgtggcc atggatatgc tcaacgaagt
     8701 gaagcggtgc cccgacgagc tgttcacaac actgtctgcc gagaagcagt cccggttcag
     8761 aatcatcagc gacgaccaca atgaagtgct gatgaagcgg agcagcgaca gattcgtgcc
     8821 tctgctgctg cagtatatcg attacggcaa gctgttcgac cacatcaggt tccacgtgaa
     8881 catgggcaag ctgagatacc tgctgaaggc cgacaagacc tgcatcgacg gccagaccag
     8941 agtcagagtg atcgagcagc ccctgaacgg cttcggcaga ctggaagagg ccgagacaat
     9001 gcggaagcaa gagaacggca ccttcggcaa cagcggcatc cggatcagag acttcgagaa
     9061 catgaagcgg gacgacgcca atcctgccaa ctatccctac atcgtggaca cctacacaca
     9121 ctacatcctg gaaaacaaca aggtcgagat gtttatcaac gacaaagagg acagcgcccc
     9181 actgctgccc gtgatcgagg atgatagata cgtggtcaag acaatcccca gctgccggat
     9241 gagcaccctg gaaattccag ccatggcctt ccacatgttt ctgttcggca gcaagaaaac
     9301 cgagaagctg atcgtggacg tgcacaaccg gtacaagaga ctgttccagg ccatgcagaa
     9361 agaagaagtg accgccgaga atatcgccag cttcggaatc gccgagagcg acctgcctca
     9421 gaagatcctg gatctgatca gcggcaatgc ccacggcaag gatgtggacg ccttcatcag
     9481 actgaccgtg gacgacatgc tgaccgacac cgagcggaga atcaagag
//
