LOCUS       sequence-259679-       14824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is 
            controlled by the xylR promoter and results in improved conjugation 
            efficiency. Induction with xylose results in a pyrE mutant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14824)
  AUTHORS   Bhattacharjee D, Sorg JA
  TITLE     Factors and Conditions That Impact Electroporation of Clostridioides
            difficile Strains.
  JOURNAL   mSphere. 2020 Mar 4;5(2):e00941-19. doi: 10.1128/mSphere.00941-19.
  PUBMED    32132157
REFERENCE   2  (bases 1 to 14824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/mSphere"; 
            journalName: "mSphere"; date: "2020-03-4- 4"; volume: "5"; issue: 
            "2"; pages: "e00941-19"
COMMENT     Sequence Label: pKM197
FEATURES             Location/Qualifiers
     source          1..14824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3574..7680
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3574..7677
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(7690..7707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7690..7706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7699..7721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_RNA        12980..13055
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     rep_origin      13253..13841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13742..13761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13995..14012
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgcga cgtgattttt aatgggtaat ttaagtgtga
      121 aatttggaag tgtagaattt aaaaatccag taataatggc gtctggaact tttggatttg
      181 gaaaagaata taatgagatt tatgatatac aaaaacttgg tggtataagt agcaagggtc
      241 ttactttaaa taaaaaacct ggtaacaatg gtatgagagt tcatgagaca ccatccggta
      301 tgatgaatag tgttgggctt gaaaaccctg gagttcaagg atttatagac tatgaattac
      361 ctttttttag taaactagac ttagtcagaa tagctaatgt tggaggtggg acattagaag
      421 actacttatt aggggttcaa atgttaaatg acaagcctat agacattatt gaattaaata
      481 tatcttgccc aaatgtcaaa gctggtggaa tggcttttgg tataaaaaat gaagtggcaa
      541 gagaagttgt tcgtgaggta agaaacataa cgaaactgcc tttagtaata aaattatcac
      601 ccaatgcaga agatattgta ggaatggcaa aagtttgtga agaagaaggt gctgatggag
      661 tttcattagt taatactttt aaagcaatgg caattgatat aaaaaataga agaccagtat
      721 ttgaaaatgt atatgctgga ttatctggac cagctataaa acctatagct ttgagaatgg
      781 tacatgaagt gtgtaaaaat gtaaatatac ctgtaatggg aatgggcgga ataactaaag
      841 ccacagatgc tatagagttt ataatggcag gagctacttg tatccaagtt ggaactgcaa
      901 attttatgaa tcctagaata ggtattgaaa taatagatgg gattaatgaa tttatggata
      961 gagaaggaat aaaaagttta gacgaaataa gaggaattat ataaggtaga ataaatagta
     1021 ttttaaagat aaaaaatata gattaaataa acaataattt tataacatta acatggagga
     1081 aaaataatta aaaacttaat tatttatagt gttacttaaa aaatgtaaat tttttagagt
     1141 aaaaatttgt aatataacat ataacttaaa aataaaaagc agttagcctt ataaatgaag
     1201 gttagctgtt ttttttgtta aaaatatatt tacaaaataa gagatagtag cagtacaata
     1261 taagtataag gtaaaagtat tatatattag ggaggggtaa gtatgaaaaa tcaattaaat
     1321 gaaaatcata aaaaggttgt attttttgga gtaggtgctg taggagctac ttttgcagaa
     1381 caatttttta actctaaata tgattttaaa attctttgtg ataatgaaag aaagaaaaga
     1441 tatttagaag aaggatttat aataaatgga aaaaggtatg attttaatta tgtaactaaa
     1501 gatgagtata aacaagaggc tgattttata attataggtc taaaatataa taatttaaaa
     1561 gaaaatataa aagaattaga tggattagtt ggaaaaaata cagttataat gtctctgcta
     1621 aatggagttg atagcgaaga gataatagga gaaagatttg gaattggaaa aatggtatat
     1681 tcatatgtta ccaatataga tgcaaagaaa atcaataata atattataca cactactaat
     1741 gggataattg tatttggtaa caaagataat agtgaagata gaaaaactaa tataataact
     1801 gaagtctttg atgatgtaaa tatagaatat attttatcaa aagatattca acgagatatg
     1861 tggtggaagt acatggttaa tattggtgta aatcaaactt cagctatact tggtgcacct
     1921 tatggagttt ttcagagttc tgagcattta agagaattag caaaatctgc aatgagggaa
     1981 gttgttgcta tagcacaagc aaaagacata tctcttacag aagatgatgt tgaaaattca
     2041 ttacatagaa tactagaaca ttcaaaagaa ggaagaacat caatgcttct cgagctagca
     2101 taaaaataag aagcctgcat ttgcaggctt cttattttta tggtacgtat aatttaaagg
     2161 gttatggctg tatatagtca taacccttat atttcattaa ttaaagttaa attttgtata
     2221 ttcaaaaagc tcttgatatt tacggcagag ccaccatata gagatgcctc agaccctaat
     2281 gatgaaattt ctatatttat tcctttgtta aaagagctaa ctaacatatc ttttgtaatc
     2341 tgtaatatct cagggatatc actaattatt tggctattta aatatattat ttcaggagca
     2401 taagttgtaa tagcattatt tatagctatt gttaagtaac tacaaaactc atgaataact
     2461 ttttggcatt ctggttatct tcataatata gttgctttac tatatcagaa tctattttag
     2521 gaatattttc taaagaagaa agctgttcaa ataccttttt ttctgaacaa tactgttcta
     2581 aacagccacg atttccacaa ggacatagtt taccattagg catgatgata gtgtgaccaa
     2641 tttctccact cataccattt ctgccactat ataatttatt atttattata atgcctgaac
     2701 caaatccact gtgaatactg agactaagta gtgagttatg tacagttgaa aaagtattct
     2761 cagctaaagc tgttaagttt gcttcatttt ctatgtgaat aggaaagtca tactttttgc
     2821 taaggatgct atataagtca atctcattaa gattgtaata tggagtaaac aatactttat
     2881 tttcacaagt aatcccatgt atagccaaag taagaccaat taccttataa ggagtgtcta
     2941 ttttggatat gttataacta tttataattt catctatcaa ttgtataaca ttgtctttac
     3001 ttacttgtat atctgttaat ttcttagaat ttataatagt tccatctaag taagatagag
     3061 aagaaaatat atagtcgtat ccaatgtcca tacttaaaga aatccctgca catttattaa
     3121 atactaataa tatgggtttt cttccgccac tatgagtact atttccaatt cctatttcat
     3181 gaactagaga ttcatcaata agttttttgg taatagcgga tatagttgct ttattcaatc
     3241 ctatagtaga agctatactt gccctagaaa taggaccatt ttttataatt tgttcaagta
     3301 ccaatctttc attcatttct cgaatagtgt atttatcagt aaccaatttg atattcctcc
     3361 ttaaaataat attgtaatac tttttacaca aaaataaaag gttattttgc attgacaaag
     3421 ataattaaat attttattat tagttcataa gttagtttaa tatactaaca aaaataaagc
     3481 aagtaaaata tacctaaaat ataaaaaaat taggatagga aaacgatagt tatgaagtgg
     3541 cattcaagga gggtaaagag gagagttaat taaatggata aaaaatatag tataggatta
     3601 gatataggaa caaatagtgt aggatgggca gtaataactg atgaatataa agtaccaagt
     3661 aaaaaattta aagtattagg aaatacagat agacatagta taaaaaaaaa tttaataggt
     3721 gcattacttt ttgatagtgg tgaaacagca gaagcaacaa gacttaaaag aacagcaaga
     3781 agaagatata ctagaagaaa aaatagaata tgttatttac aagaaatatt tagtaatgaa
     3841 atggcaaaag tagatgatag tttttttcat agattagaag aaagtttttt agtagaagaa
     3901 gataaaaaac acgaaagaca tccaatattt ggaaatatag tagatgaagt agcatatcat
     3961 gaaaaatatc caacaatata tcatttaaga aaaaaacttg tagatagtac agataaagca
     4021 gatttaagac ttatatatct tgcattagca catatgataa aatttagagg acattttctt
     4081 atagaaggtg atttaaatcc agataatagt gatgtagata aactttttat acaattagta
     4141 caaacatata atcaattatt tgaagaaaat ccaataaatg caagtggtgt agatgcaaaa
     4201 gcaatattaa gtgcaagatt aagtaaaagt agaagattag aaaatcttat agcacaatta
     4261 cctggagaaa aaaaaaatgg attatttggt aatcttatag ctttaagttt aggattaaca
     4321 ccaaatttta aatcaaattt tgatcttgca gaagatgcaa aacttcaatt aagtaaagat
     4381 acatatgatg atgatttaga taatttactt gcacaaatag gtgatcaata tgcagattta
     4441 tttcttgcag caaaaaatct ttctgatgca atacttttaa gtgatatact tagagtaaat
     4501 acagaaataa caaaagcacc attatctgca agtatgataa aaagatatga tgaacatcat
     4561 caagatttaa cattacttaa agcattagta agacaacaat taccagaaaa atataaagaa
     4621 attttttttg atcaaagtaa aaatggatat gcaggatata tagatggtgg tgcaagtcaa
     4681 gaagaatttt ataaatttat taaaccaata ttagaaaaaa tggatggaac agaagaatta
     4741 ttagtaaaac ttaatagaga agatttactt agaaaacaaa gaacatttga taatggaagt
     4801 ataccacatc aaatacattt aggtgaatta catgcaatat taagaagaca agaagatttt
     4861 tatccatttc ttaaagataa tagagaaaaa attgaaaaaa tacttacatt tagaatacca
     4921 tattatgtag gaccattagc aagaggaaat agtagatttg cttggatgac aagaaaaagt
     4981 gaagaaacaa taacaccatg gaattttgaa gaagtagtag ataaaggagc atctgcacaa
     5041 agttttatag aaagaatgac aaattttgat aaaaatcttc caaatgaaaa agtattacca
     5101 aaacatagtt tattatatga atattttaca gtatataatg aattaacaaa agtaaaatat
     5161 gtaacagaag gtatgagaaa accagcattt ttaagtggtg aacaaaaaaa agctatagta
     5221 gatttattat ttaaaacaaa tagaaaagta acagtaaaac aattaaaaga agattatttt
     5281 aaaaaaattg aatgttttga tagtgtagaa atatctggtg tagaagatag atttaatgca
     5341 agtttaggaa catatcatga tttacttaaa ataataaagg ataaagattt tttagataat
     5401 gaagaaaatg aagatatttt agaagatata gtattaacat taactttatt tgaagataga
     5461 gaaatgatag aagaaagatt aaaaacttat gctcatttat ttgatgataa agtaatgaaa
     5521 caacttaaaa gaagaagata tacaggttgg ggaagattaa gtagaaaact tataaatgga
     5581 ataagagata aacaaagtgg aaaaacaata ttagattttc ttaaaagtga tggatttgca
     5641 aatagaaatt ttatgcaatt aatacatgat gatagtttaa catttaaaga agatattcaa
     5701 aaagcacaag taagtggaca aggtgatagt ttacatgaac atatagcaaa tttagcagga
     5761 tctccagcaa taaaaaaagg tatattacaa acagtaaaag tagttgatga attagtaaaa
     5821 gtaatgggta gacataaacc agaaaatata gttatagaaa tggcaagaga aaatcaaaca
     5881 acacaaaaag gacaaaaaaa tagtagagaa agaatgaaaa gaatagaaga aggtataaaa
     5941 gaattaggaa gtcaaatact taaagaacat ccagtagaaa atacacaact tcaaaatgaa
     6001 aaactttatt tatattattt acaaaatgga agagatatgt atgtagatca agaattagat
     6061 ataaatagat taagtgatta tgatgtagat catatagtac cacaaagttt tcttaaagat
     6121 gattctatag ataataaagt attaacaaga tctgataaaa atagaggtaa aagtgataat
     6181 gtaccaagtg aagaagttgt aaaaaaaatg aaaaattatt ggagacaatt acttaatgca
     6241 aaacttataa cacaaagaaa atttgataat cttacaaaag cagaaagagg tggattatct
     6301 gaattagata aagctggatt tataaaaaga caattagtag aaactagaca aataacaaaa
     6361 catgtagcac aaatattaga tagtagaatg aatacaaaat atgatgaaaa tgataagctt
     6421 ataagagaag taaaagttat aacattaaaa agtaaattag taagtgattt tagaaaagat
     6481 tttcaatttt ataaagtaag agaaataaat aattatcatc atgcacatga tgcatatctt
     6541 aatgcagtag taggaacagc attaataaaa aaatatccta aattagaatc tgaatttgta
     6601 tatggagatt ataaagtata tgatgtaaga aaaatgatag caaaaagtga acaagaaata
     6661 ggaaaagcaa cagcaaagta ctttttttat tctaatataa tgaatttttt taagacagaa
     6721 ataactttag caaatggtga aataagaaaa agacctttaa tagaaactaa tggtgaaaca
     6781 ggtgaaatag tatgggataa aggtagagat tttgcaacag taagaaaagt attaagtatg
     6841 ccacaagtaa atatagtaaa aaaaacagaa gtacaaactg gaggatttag taaagaaagt
     6901 atacttccaa aaagaaattc agataaactt attgcaagaa aaaaagattg ggatccaaaa
     6961 aaatatggtg gatttgatag tccaacagta gcatatagtg tattagtagt agcaaaagta
     7021 gaaaaaggta aatctaaaaa acttaaaagt gtaaaagaat tacttggaat aacaataatg
     7081 gaaagaagta gttttgaaaa aaatcctatt gattttttag aagcaaaagg atataaagaa
     7141 gttaaaaaag atttaataat aaaacttcca aaatattctt tatttgaatt agaaaatgga
     7201 agaaaaagaa tgttagcaag tgctggtgaa ttacaaaaag gtaatgaatt agcattacca
     7261 tctaaatatg taaattttct ttatttagca agtcattatg aaaaacttaa aggatctcct
     7321 gaagataatg aacaaaaaca attatttgta gaacaacata aacattattt agatgaaata
     7381 attgaacaaa taagtgaatt ttctaaaaga gtaatattag cagacgcaaa tttagataaa
     7441 gtattatctg cttataataa acatagagat aaaccaataa gagaacaagc agaaaatata
     7501 atacatcttt ttacattaac aaatttaggt gcaccagcag catttaaata ttttgataca
     7561 acaatagata gaaaaagata tacaagtaca aaagaagtat tagacgcaac attaatacat
     7621 caaagtataa caggattata tgaaacaaga atagatttat ctcaattagg tggtgattaa
     7681 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7741 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7801 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct agcataaaaa
     7861 taagaagcct gcatttgcag gcttcttatt tttatggcgc gcccgccctt aagtctaaaa
     7921 attaggggag atgtaaggat ttgggaaaaa tagaagatgt tataatcata aatatggtat
     7981 tcgtaggctt aaagtcaaaa aggaggtgaa atataaatag atttttagct aaattaagta
     8041 agaaatagga ggagatttat tgaacaaaaa attagaaaaa ccatttgtat ataagagaga
     8101 gtacgatttg actggatatg atgttgaaat tttacaaaaa tatgagttag aacaagcaat
     8161 atatgtttat gttgggagta gttgtgcata taacatgaga gctagaagta gtaaatggag
     8221 ataccatata agaacaaata ataagtctat atgttgtaac attaaaaatt ttatacataa
     8281 cttggaattg ttttataaaa tggaattaaa gttgtcagat aatattatta atgataagct
     8341 atactatagc aatatagcag agtttgaaga atttgaaaca ctagaaaaag ctagagaggt
     8401 agaaagtact attataagtc aatatcaatt tttagattct ataaatcaca tgttaaaaca
     8461 aaaaataatt ttattgagta ataaggatag tgtgttaaac ataactaaaa atggaaatac
     8521 aaattatttg aaagtaaaaa ataaatacat agaaaaacat aagaacaagc caataatgag
     8581 ataccatatc aactgtcaat tcaatacaga tggaagtgtc aaaagtatta cacaggagtt
     8641 tgaaccaata ttggaattaa acaaaaaaaa taccctaagc cgaccaagca gagtattttt
     8701 aaaataatat tttaagataa caacaaaatg agataatact actagacaat gacaactcaa
     8761 ctaccaattg agtttatgga gctaccaact ccaatatcgg tctaactgat taagtatctg
     8821 tagttatata ataatattgc tatcaatttt agcatcttaa caatattatt atacatacta
     8881 agctaaaatt attcaatagt tgtaaaagtt gattagtcaa taagtatata tttaatgtag
     8941 tgttatctct taaaaaaact agataaggag ataataaata tatggaacaa ttagattcaa
     9001 aatataagtt gaaaaaattt ctaatggcag tatttagaga tggtatagga caaggaaata
     9061 atcttattga taatgaatat gttagagtat ttcaaaataa taaaagtaat agtaaacaat
     9121 tagaactcgg agaagaattt aaagaatata gtaaaacaac tttttttaaa aatatagatg
     9181 atatagtaga atttaccttc gcaaaaaata tttattatga aaatacattt tttaacctat
     9241 gtactactga tggaaaagca ggaaccaatg aaaacttaat aaatagatat gcattaggat
     9301 ttgattttga caaaaaagaa ttaggacaag gttttaatta taaagatata attaatttat
     9361 ttactaagat aggattacat tatcatatcc tagttgatag tggaaatgga ttccatgttt
     9421 atgtgctaat taataaaact aataacatta agttagtatc agaagttaca aatacattaa
     9481 taaataaatt gggtgcagat aaacaagcaa atttatctac tcaagtatta agagtacctt
     9541 atacatataa tattaaaaat actactaaac aagtaaaaat aatacaccaa gacaaaaata
     9601 tatatagata tgacatagaa aagttagcta aaaaatattg caaagatgta aaaacagtag
     9661 gtaatactaa tacaaaatat atattagata gtaagctacc aaattgtata gtagatattt
     9721 taaaaaatgg tagtaaagat ggacataaaa acctagattt gcaaaaaata gttgtgactt
     9781 taagattgag gaataaaagt ttaagtcaag taatatccgt tgctagagaa tggaactata
     9841 tatcacaaaa tagtctttca aatagtgagc tagaatatca agtcaagtat atgtatgaga
     9901 aacttaaaac ggttaatttt ggttgtactg gttgtgagtt taatagtgat tgttggaata
     9961 aaatagaatc agattttata tatagtgatg aagatacttt gttcaatatg ccacataagc
    10021 actcaaagga tttgaaatat aagaatagga aaggggttaa aataatgact ggtaatcaat
    10081 tgtttatcta taatgtgtta cttaacaata aagatagaga attaaacata gacgatataa
    10141 tggagctgat aacctataaa cgtaagaaga aagttaaaaa cattgttatg agtgaaaaga
    10201 cattaagaga aacattaaaa gaacttcaac ataatgatta tattacaaaa acaaaaggtg
    10261 ttacaaagct aggaataaaa gatacataca atgtaaaaga agttagatgt aatatagata
    10321 aacaatatac tattagttac tttgttacca tggcagtaat ttggggaata atttcaactg
    10381 aagaattaag attatatact cacatgagat ataagcaaga tttattggtc aaagatgata
    10441 aaataaaagg aaatatatta agaattaatc aagaggaatt agcaaaagat ttaggagtaa
    10501 cacagcaaag aatttcaaat atgatagaat ctttattaga tactaaaatt ttagatgtat
    10561 gggaaactaa aataaatgat agaggattta tgtactatac atatagatta aacaagtaga
    10621 tttttgatag gattagaatt gattttctag tcctattttt atgcaaaaaa actaattaat
    10681 aaaaatttct tttggtaaaa taattgtacg agaattgcaa aaaaaaaatg gcatcaaagt
    10741 attgaaatta agccgtttta aaaatttctt ttggtaaaat aattctacat atatatgtag
    10801 tatatatata tatgtttttt agagaatgta taactagaat atagagctag aatatagaga
    10861 atgtataact agaatataga gctagaatat agagaatgta taactagaat atagagctag
    10921 aatatagaga atgtataact agaatataga gctagaatat agagaatgta taactagaat
    10981 atagagctag aatatagaga atgtataact agaatataga gctagaatat agagaatgta
    11041 taactagaat atagagctag aatcctaatt agtaggtgct tttttaaaac aagttaaaaa
    11101 tcaaaaatag tattagtaag cattggaaat gctagattct aaaatagaaa agtaaaaaat
    11161 tggtgcacta tctaaactta tctatatcgc tttttccgtc gtttggttct ctagttacga
    11221 tacaggggat atgcttatat tgagttatag tactaatcag tgcttaatat agttaataaa
    11281 attatagtta ccatagttta gtaactatga tgtatgttag ttagaaactt gcatttcggc
    11341 cggccagtgg gcaagttgaa aaattcacaa aaatgtggta taatatcttt gttcattaga
    11401 gcgataaact tgaatttgag agggaactta gatggtattt gaaaaaattg ataaaaatag
    11461 ttggaacaga aaagagtatt ttgaccacta ctttgcaagt gtaccttgta cctacagcat
    11521 gaccgttaaa gtggatatca cacaaataaa ggaaaaggga atgaaactat atcctgcaat
    11581 gctttattat attgcaatga ttgtaaaccg ccattcagag tttaggacgg caatcaatca
    11641 agatggtgaa ttggggatat atgatgagat gataccaagc tatacaatat ttcacaatga
    11701 tactgaaaca ttttccagcc tttggactga gtgtaagtct gactttaaat catttttagc
    11761 agattatgaa agtgatacgc aacggtatgg aaacaatcat agaatggaag gaaagccaaa
    11821 tgctccggaa aacattttta atgtatctat gataccgtgg tcaaccttcg atggctttaa
    11881 tctgaatttg cagaaaggat atgattattt gattcctatt tttactatgg ggaaatatta
    11941 taaagaagat aacaaaatta tacttccttt ggcaattcaa gttcatcacg cagtatgtga
    12001 cggatttcac atttgccgtt ttgtaaacga attgcaggaa ttgataaata gttaacttca
    12061 ggtttgtctg taactaaaaa caagtattta agcaaaaaca tcgtagaaat acggtgtttt
    12121 ttgttaccct aagtttcata aaaataagaa gcctgcaaat gcaggcttct tatttttatg
    12181 gtttaaacgg ttttagctgg gatatcggca aaacaagcaa atactactat agaagcagtt
    12241 aaaagggcta ttttttataa tctggagcat agacaagaag ttagagaagc aattaaaaaa
    12301 gaaaatataa gaaaaattct tattattggg acatcaaaaa aaatggttaa tcaaattgta
    12361 gagagattgt caattggaag ggtctgtaag tttataaata tacaagatat aagtactaaa
    12421 tatgaaatag aaatagcaaa acaagctaga aaagaaggaa atcatgtaat tcctgttcca
    12481 gccgttgaga taaaaagtat ggcaagtggt ctcagtataa attctttgaa gagacttttt
    12541 agaaaaggaa ataataggaa tatggtagtt ttagaaaaaa caattataag accaactttt
    12601 agttatatag gaaagtttta tattagtgct gatgtaatta aacagattat tgaatatgaa
    12661 gtatacaact ttggaagcat tgatagaata aataaaatca atatagagaa ttgcaataat
    12721 tttatgaata tttttgtaag tataaatatc aatgatttag aaactataaa aaaaattgaa
    12781 gacatacaac agacaataaa aaagtctatt gagaaaatga ctttagttaa tgtacaaaaa
    12841 atagatatta acattcataa aattaaaaaa ataaacttcc tccaatgtta aaaaataaac
    12901 tgagaaaatg atatactaat ttgcaataaa gtgtgctata attaaactgt aaaggtaccg
    12961 aaaagtgatg cattgttggg ttttagagct agaaatagca agttaaaata aggctagtcc
    13021 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttctatggag aaatctagat
    13081 cagcatgatg tctgactaga cgcgtaagct ctgcaactat ttttagatgg ttgcagagct
    13141 tatttttatt ttttattaaa ctcctttttg ataatctcat gaccaaaatc ccttaacgtg
    13201 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    13261 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    13321 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    13381 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    13441 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    13501 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    13561 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    13621 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    13681 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    13741 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    13801 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    13861 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    13921 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    13981 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaggg
    14041 ccctaacatc ttctattttt cccaaatcct tacatctccc ctaattttta gacttaaggg
    14101 cgaattccaa aggacgaata tgtcgcctaa agcataatgc tctgaataag aatagaaacc
    14161 atagtcctca tgaagaaaat aggacagttt cagttgtaag atgttttcga ccacctgctg
    14221 tacgtctgtt gtcggaaagc gaatccttac ataatcaaac agcatttcaa ggggagcgtc
    14281 gggattgaag cgttccagag cttcccaaag ggactgctgt aaatcctctg atggcttgac
    14341 ttttcctgtt tcaatatcgc ttagatactg ccttgtaata ccagtcgcaa cagctaaacg
    14401 gttttgagat agtccataag ccaagcgttt ttcttttaaa tgctgtaacc aagtttgttc
    14461 attcagtaaa aatccctcca atcaaaaagg cgtatgtcaa cttttaaagc ccatttgaca
    14521 tacgctgaaa ttttgtaaat cccttgtaac caaaggattt tctaatgttt ttttgactgt
    14581 ttcctgtcga tttgtacccc cctgttagat acggggggtt aagtgctggc gtggctattg
    14641 ccacaccagc cagcaagatc agtccacacc tgcgacttcc gcttcgcacg tcgcctgcgt
    14701 ggactgtctg ctgttggata actttttaat ttcctccaag aaatcatatc cttttggtac
    14761 aaggggagta taaaagaatt ctgcagatta cctaataatt tatctacatt ccctttaggg
    14821 gccc
//
