LOCUS       sequence-25968-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for nuclear targeting of domains (N-term HA , 
            C-term NLS, myc tags).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-HA-myc-nuc (w557-1)
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             127..150
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             214..234
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             262..282
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             295..324
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(376..475)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(587..606)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(665..684)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt ttttataatg
       61 ccaactttgt acaaaaaagc aggctccacc atggcctacc cctacgacgt gcccgactat
      121 gccaaactgg aagttctgtt ccaggggccc acatgtaaca tggcccaggt gcagctgcag
      181 gtcgacctcg agatcaaacg ggcggccgca gatccaaaaa agaagagaaa ggtagatcca
      241 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg tagatacggc cgcagaacaa
      301 aaactcatct cagaagagga tctgaatggg gccgcatagt ctagaagctc gctgatccgg
      361 taccgaatta tctagaccca gctttcttgt acaaagttgg cattataaga aagcattgct
      421 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
      481 cagctgcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
      541 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
      601 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
      661 tatgggtata aatgggctcg cgataatgtc gggcattcag gtgcgacatc tancgcttgt
      721 atggaagccc gtcg
//
