LOCUS       sequence-259685-       11060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Sec23B as a fusion with mGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Hughes H, Stephens DJ
  TITLE     Sec16A defines the site for vesicle budding from the endoplasmic 
            reticulum on exit from mitosis.
  JOURNAL   J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8. Epub 2010 Nov 2.
  PUBMED    21045114
REFERENCE   2  (bases 1 to 11060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2010-12-1"; volume: "123(Pt 23)"; pages: "4032-8. Epub
            2010 Nov 2"
COMMENT     Sequence Label: pLVXPuro-mGFP-Sec23B
FEATURES             Location/Qualifiers
     source          1..11060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2828..2837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2834..2836,2837..2839,2840..3550)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2879..2900)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3140..3159)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3487..3508
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5895..6394
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5930..5953)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6327..6346
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6415..7014
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6415..6434)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6911..6931
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7028..7616
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7081..7101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7499..7510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7823..8456
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8585..8601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8585..8601)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8598..8620)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8609..8625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8633..8639,8640..8657,8658..8663))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8678..8699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8816..8833)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8987..9575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9067..9086)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9746..10537,10538..10606))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10369..10388
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10607..10711)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10759..10893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10796..10815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10850..10869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgac
     2821 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2881 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2941 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3001 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     3061 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3121 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3181 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3241 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     3301 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     3361 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3421 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     3481 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3541 gctgtacaag ctcgaggaat tcatggcgac atacctggag ttcatccagc agaatgaaga
     3601 acgggatggt gtgcgtttta gttggaacgt gtggccttcc agccggctgg aggctacaag
     3661 aatggttgta cccctggctt gtctccttac tcctttgaaa gaacgtccag acctacctcc
     3721 tgtacaatat gaacctgtgc tttgcagcag gccaacttgt aaagctgttc tcaacccact
     3781 ttgtcaggtt gattatcgag caaaactttg ggcctgtaat ttctgttttc aaagaaatca
     3841 gtttcctcca gcttatggag gcatatctga ggtgaatcaa cctgccgaat tgatgcccca
     3901 gttttctaca attgagtacg tgatacagcg aggtgctcag tcccctctga tctttctcta
     3961 tgtggttgac acatgcctgg aggaagatga ccttcaagca ctcaaagagt ccctgcagat
     4021 gtccctgagt cttcttcctc cagatgctct ggtgggtctg atcacatttg gaaggatggt
     4081 gcaggttcat gagctaagct gtgaaggaat ctccaaaagt tatgtcttcc gagggaccaa
     4141 ggatttaact gcaaagcaaa tacaggatat gttgggcctg accaagccag ccatgcccat
     4201 gcagcaagca cgacctgcac aaccacagga gcaccctttt gcttcaagca gatttctgca
     4261 gcctgttcac aagattgata tgaacctcac tgatcttctt ggggagctac agagggaccc
     4321 atggccagta actcagggga agagaccttt gcgatccact ggtgtggctt tgtccattgc
     4381 tgttggcttg ctggagggca cttttccaaa cacaggagcc aggatcatgc tgtttactgg
     4441 aggtccccct acccaagggc ctggcatggt ggttggagat gaattaaaga ttcctattcg
     4501 ttcttggcat gatattgaga aagataatgc acgattcatg aaaaaggcaa ccaagcacta
     4561 tgagatgctt gctaatcgaa cagctgcaaa tggtcactgc attgatattt atgcttgtgc
     4621 ccttgatcaa actggacttt tggagatgaa gtgttgtgca aatcttactg gaggctacgt
     4681 ggtaatggga gattctttca acacttctct cttcaagcag acattccaaa gaatctttac
     4741 taaagatttt aatggagatt tccgaatggc atttggtgct actttggacg taaagacctc
     4801 tcgggaactg aagattgcag gagccattgg tccatgcgta tctctgaatg tgaaaggact
     4861 gtgtgtgtca gaaaatgagc ttggtgttgg tggcacgagt cagtggaaaa tctgtggcct
     4921 agatcctaca tctacacttg gcatctattt tgaagttgtc aatcagcaca acaccccgat
     4981 cccccaagga ggcagaggag ccatccagtt tgtcacgcat tatcagcact ccagcaccca
     5041 gagacgcatc cgcgtgacca ccatcgcccg aaattgggca gatgtacaga gtcagctcag
     5101 gcacatagaa gcagcatttg accaggaggc tgcggcagtg ttgatggcac ggcttggggt
     5161 gttccgagcg gagtcagagg aggggcccga tgtgctccgg tggctggacc gacaactcat
     5221 ccgactgtgt caaaagtttg gacagtataa caaagaagac cccacttctt ttaggttatc
     5281 agattccttt tctctatatc ctcagtttat gttccatctg agaagatctc catttcttca
     5341 agtgtttaac aacagtcctg atgagtcgtc atattacaga catcattttg cccggcagga
     5401 cctgacccag tccctcatca tgatccagcc cattctctac tcttactcct ttcatgggcc
     5461 accagagcca gtactcttgg atagcagcag cattctagct gacagaattt tgctgatgga
     5521 tactttcttt caaattgtca tttatcttgg tgagaccata gcccagtggc gtaaagctgg
     5581 ctaccaggac atgcccgagt atgaaaactt caagcacctt ctgcaggcac cactggatga
     5641 tgctcaagaa attctgcaag cacgcttccc gatgccacgt tacatcaaca cggagcatgg
     5701 aggcagtcag gctcgattcc ttttgtccaa agtgaaccca tctcagacac acaataacct
     5761 gtatgcttgg ggacaggaaa ctggagcacc catcctaact gatgatgtta gcctgcaggt
     5821 gttcatggac catttgaaga agctggctgt ctccagtgcc tgttgaggat cccgcgactc
     5881 tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     5941 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     6001 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     6061 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     6121 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     6181 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     6241 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     6301 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     6361 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
     6421 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6481 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6541 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6601 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6661 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6721 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6781 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6841 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6901 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6961 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt
     7021 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     7441 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7501 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7561 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7621 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     7681 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     7741 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7801 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc
     7861 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     7921 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     7981 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     8041 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     8101 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     8161 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     8221 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     8281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8461 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8521 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat
     8581 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8641 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8701 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8761 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8821 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8881 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8941 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9001 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9061 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9121 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9181 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9241 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9301 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9361 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9421 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9481 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9601 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9661 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10021 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10741 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10801 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10861 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    10921 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
    10981 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    11041 tgtactacaa acttagtagt
//
