LOCUS       sequence-259693-       12555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the E.coli RaPID labeling protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12555)
  AUTHORS   Ramanathan M, Majzoub K, Rao DS, Neela PH, Zarnegar BJ, Mondal S, 
            Roth JG, Gai H, Kovalski JR, Siprashvili Z, Palmer TD, Carette JE, 
            Khavari PA
  TITLE     RNA-protein interaction detection in living cells.
  JOURNAL   Nat Methods. 2018 Feb 5. pii: nmeth.4601. doi: 10.1038/nmeth.4601.
  PUBMED    29400715
REFERENCE   2  (bases 1 to 12555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Feb 5. pii: nmeth.4601. doi: 10.1038/nmeth.4601."
COMMENT     Sequence Label: E.coli RaPID plasmid
FEATURES             Location/Qualifiers
     source          1..12555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..573
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(168..185)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     395..414
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             576..1175
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1075..1095
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1185..1238
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1245..1254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1251..1961
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1396..1414)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1664..1683
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1974..2562
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2027..2047)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2445..2456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2769..3002
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(3103..3120)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3109..3333
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3379..3807
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3466..3485)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3676..3697
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3816..3836)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3821..4150
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4001..4136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4063..4082
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4199..5224
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4199..5224
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    5354..5475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5524..5540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5524..5540)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5537..5559)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5548..5564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5572..5578,5579..5596,5597..5602))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5617..5638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5755..5772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5926..6514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6006..6025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6685..7476,7477..7545))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7308..7327
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7546..7650)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7698..7832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             8000..8633
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8680..8805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9302..9535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9720..9764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10062..10179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10244..10618)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      10615..10624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10628..10646)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(10638..10685)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        10790..11093
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11094..11297
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11248..11268
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11294..11318
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11491..11517
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             11527..11553
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(11554..11901,11902..11904,11905..12513)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
       61 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgtgggcccg
      121 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
      181 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
      241 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
      301 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
      361 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
      421 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
      481 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
      541 ggacgtggtt ttcctttgaa aaacacgata ataccatggc caccgagtac aagcccacgg
      601 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg
      661 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg
      721 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
      781 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
      841 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
      901 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
      961 tcggcgtatc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     1021 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     1081 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     1141 cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc gggagagggc agaggaagtc
     1201 tgctaacatg cggtgacgtc gaggagaatc ctggcccacc ggtcgccacc atggtgagca
     1261 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     1321 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1381 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     1441 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     1501 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1561 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     1621 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1681 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1741 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1801 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1861 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1921 gccgccactc caccggcggc atggacgagc tgtacaagta agaattcgtt aacaatcaac
     1981 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2041 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2101 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2161 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2221 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2281 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2341 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     2401 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     2461 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     2521 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
     2581 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     2641 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     2701 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     2761 gaggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     2821 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     2881 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     2941 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     3001 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     3061 agagagtgag aggccttgac attgtttaaa cccgctgatc agcctcgact gtgccttcta
     3121 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3181 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3241 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3301 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     3361 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3421 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3481 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3541 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3601 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3661 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3721 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3781 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     3841 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3901 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3961 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4021 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4081 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4141 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgat
     4201 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     4261 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     4321 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     4381 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     4441 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     4501 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     4561 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     4621 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     4681 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     4741 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     4801 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     4861 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     4921 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     4981 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     5041 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     5101 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     5161 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     5221 atagcacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5281 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5341 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5461 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     5521 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5581 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5641 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6061 cctgccactt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6361 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7681 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7741 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7801 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     7861 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
     7921 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
     7981 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
     8041 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     8101 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     8161 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     8221 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     8281 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     8341 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     8401 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     8461 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8521 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8581 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     8641 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     8701 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     8761 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8821 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8881 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8941 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     9001 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     9061 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     9121 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
     9181 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     9241 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     9301 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     9361 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     9421 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     9481 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     9541 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     9601 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     9661 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     9721 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     9781 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     9841 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     9901 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     9961 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
    10021 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
    10081 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
    10141 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
    10201 agagatccag tttggttagt accgggcccg ctctagtccg gaatcagtcc tgctcctcgg
    10261 ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc ccccacggct
    10321 gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg gacacgacct
    10381 ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt
    10441 ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc cggaccacac
    10501 cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc cagaactcga
    10561 ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac ttggccatgg
    10621 tggccctcct atagtgagtc gtattatact atgccgatat actatgccga tgattaattg
    10681 tcaacacgtg ctgcaggtcc gaggttctag acgtattacc gccatgcatt agttattaat
    10741 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
    10801 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
    10861 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
    10921 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
    10981 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
    11041 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
    11101 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
    11161 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
    11221 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
    11281 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
    11341 gttttgacct ccatagaaga caccgactct actagaggat ccatgaacgc acgaacacga
    11401 cgacgtgagc gtcgcgctga gaaacaagct caatggaaag ctgcaaactc aggaggcggt
    11461 gggtctggtg gcgggagcgg ccgcctgcag ctgccccccc tggagcgcct gaccctggac
    11521 ctcgagtacc catacgatgt gcctgactat gctaaggaca acaccgtgcc cctgaagctg
    11581 atcgccctgc tggccaacgg cgagttccac tctggcgagc agctgggaga gaccctggga
    11641 atgagcagag ccgccatcaa caagcacatc cagacactga gagactgggg agtggacgtg
    11701 ttcaccgtgc ctggcaaggg ctacagcctg cctgagccta tccagctgct gaacgccaag
    11761 cagatcctgg gacagctgga tggcggaagc gtggccgtgc tgcctgtgat cgactccacc
    11821 aatcagtacc tgctggacag aatcggagag ctgaagtccg gcgacgcctg catcgccgag
    11881 taccagcagg ctggcagagg aggcagagga cggaagtggt tcagcccatt cggagccaac
    11941 ctgtacctgt ccatgttctg gagactggag cagggacctg ctgctgccat cggactgagt
    12001 ctggtgatcg gaatcgtgat ggccgaggtg ctgagaaagc tgggagccga caaggtgaga
    12061 gtgaagtggc ctaatgacct gtacctccag gaccgcaagc tggctggcat cctggtggag
    12121 ctgacaggca agacaggcga tgccgctcag atcgtgatcg gagccggaat caacatggcc
    12181 atgagaagag tggaggagag cgtggtgaac cagggctgga tcaccctgca ggaggctggc
    12241 atcaacctgg accggaacac cctggccgcc atgctgatca gagagctgag agccgctctg
    12301 gagctgttcg agcaggaggg actggctcct tacctgagca gatgggagaa gctggacaac
    12361 ttcatcaaca gacctgtgaa gctgatcatc ggcgacaagg aaatcttcgg catctccaga
    12421 ggaatcgaca agcagggagc tctgctgctg gagcaggacg gaatcatcaa gccctggatg
    12481 ggcggagaaa tctccctgag aagcgcagag aagtgagtcg agtctagagg gcccgacgcg
    12541 tctggccacc ggtcg
//
