LOCUS       sequence-259760-        9176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for human codon optimized 
            enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding 
            E174R/N282A/S542R/K548R substitutions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)
FEATURES             Location/Qualifiers
     source          1..9176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          288..1304
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1228..1250
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1312..1331
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1403..5323
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     ALAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5043..5052
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5324..5371
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5378..5458
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5548..5567)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5613..5668
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5667..5686)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6029..6045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6029..6045)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6042..6064)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6053..6069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6077..6083,6084..6101,6102..6107))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6122..6143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6202..6397
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6248..6383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6403..6537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6440..6459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6494..6513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6605..6622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6776..7364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6856..6875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7535..8326,8327..8395))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8158..8177
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8396..8500)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8531..8910
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
       61 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
      121 gggggggggg gsgcgcggsg gggggggggg gggggggcgr ggggsggggs ggggcgaggc
      181 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
      241 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
      301 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      361 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      421 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      481 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      541 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      601 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
      661 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
      721 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
      781 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
      841 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
      901 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
      961 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1021 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1081 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1141 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1201 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1261 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1321 tggttattgt gctgtctcat cattttggca aagaattctg cagtcgacgg taccgcgggc
     1381 ccgggatcca ccggtcgcca ccatgacaca gttcgagggc tttaccaacc tgtatcaggt
     1441 gagcaagaca ctgcggtttg agctgatccc acagggcaag accctgaagc acatccagga
     1501 gcagggcttc atcgaggagg acaaggcccg caatgatcac tacaaggagc tgaagcccat
     1561 catcgatcgg atctacaaga cctatgccga ccagtgcctg cagctggtgc agctggattg
     1621 ggagaacctg agcgccgcca tcgactccta tagaaaggag aaaaccgagg agacaaggaa
     1681 cgccctgatc gaggagcagg ccacatatcg caatgccatc cacgactact tcatcggccg
     1741 gacagacaac ctgaccgatg ccatcaataa gagacacgcc gagatctaca agggcctgtt
     1801 caaggccgag ctgtttaatg gcaaggtgct gaagcagctg ggcaccgtga ccacaaccga
     1861 gcacgagaac gccctgctgc ggagcttcga caagtttaca acctacttct ccggctttta
     1921 tagaaacagg aagaacgtgt tcagcgccga ggatatcagc acagccatcc cacaccgcat
     1981 cgtgcaggac aacttcccca agtttaagga gaattgtcac atcttcacac gcctgatcac
     2041 cgccgtgccc agcctgcggg agcactttga gaacgtgaag aaggccatcg gcatcttcgt
     2101 gagcacctcc atcgaggagg tgttttcctt ccctttttat aaccagctgc tgacacagac
     2161 ccagatcgac ctgtataacc agctgctggg aggaatctct cgggaggcag gcaccgagaa
     2221 gatcaagggc ctgaacgagg tgctggccct ggccatccag aagaatgatg agacagccca
     2281 catcatcgcc tccctgccac acagattcat ccccctgttt aagcagatcc tgtccgatag
     2341 gaacaccctg tctttcatcc tggaggagtt taagagcgac gaggaagtga tccagtcctt
     2401 ctgcaagtac aagacactgc tgagaaacga gaacgtgctg gagacagccg aggccctgtt
     2461 taacgagctg aacagcatcg acctgacaca catcttcatc agccacaaga agctggagac
     2521 aatcagcagc gccctgtgcg accactggga tacactgagg aatgccctgt atgagcggag
     2581 aatctccgag ctgacaggca agatcaccaa gtctgccaag gagaaggtgc agcgcagcct
     2641 gaagcacgag gatatcaacc tgcaggagat catctctgcc gcaggcaagg agctgagcga
     2701 ggccttcaag cagaaaacca gcgagatcct gtcccacgca cacgccgccc tggatcagcc
     2761 actgcctaca accctgaaga agcaggagga gaaggagatc ctgaagtctc agctggacag
     2821 cctgctgggc ctgtaccacc tgctggactg gtttgccgtg gatgagtcca acgaggtgga
     2881 ccccgagttc tctgcccggc tgaccggcat caagctggag atggagcctt ctctgagctt
     2941 ctacaacaag gccagaaatt atgccaccaa gaagccctac tccgtggaga agttcaagct
     3001 gaactttcag atgcctacac tggccagagg ctgggacgtg aatagagaga agaacaatgg
     3061 cgccatcctg tttgtgaaga acggcctgta ctatctgggc atcatgccaa agcagaaggg
     3121 caggtataag gccctgagct tcgagcccac agagaaaacc agcgagggct ttgataagat
     3181 gtactatgac tacttccctg atgccgccaa gatgatccca aagtgcagca cccagctgaa
     3241 ggccgtgaca gcccactttc agacccacac aacccccatc ctgctgtcca acaatttcat
     3301 cgagcctctg gagatcacaa aggagatcta cgacctgaac aatcctgaga aggagccaaa
     3361 gaagtttcag acagcctacg ccaagaaaac cggcgaccag aagggctaca gagaggccct
     3421 gtgcaagtgg atcgacttca caagggattt tctgtccaag tataccaaga caacctctat
     3481 cgatctgtct agcctgcggc catcctctca gtataaggac ctgggcgagt actatgccga
     3541 gctgaatccc ctgctgtacc acatcagctt ccagagaatc gccgagaagg agatcatgga
     3601 tgccgtggag acaggcaagc tgtacctgtt ccagatctat aacaaggact ttgccaaggg
     3661 ccaccacggc aagcctaatc tgcacacact gtattggacc ggcctgtttt ctccagagaa
     3721 cctggccaag acaagcatca agctgaatgg ccaggccgag ctgttctacc gccctaagtc
     3781 caggatgaag aggatggcac accggctggg agagaagatg ctgaacaaga agctgaagga
     3841 tcagaaaacc ccaatccccg acaccctgta ccaggagctg tacgactatg tgaatcacag
     3901 actgtcccac gacctgtctg atgaggccag ggccctgctg cccaacgtga tcaccaagga
     3961 ggtgtctcac gagatcatca aggataggcg ctttaccagc gacaagttct ttttccacgt
     4021 gcctatcaca ctgaactatc aggccgccaa ttccccatct aagttcaacc agagggtgaa
     4081 tgcctacctg aaggagcacc ccgagacacc tatcatcggc atcgatcggg gcgagagaaa
     4141 cctgatctat atcacagtga tcgactccac cggcaagatc ctggagcagc ggagcctgaa
     4201 caccatccag cagtttgatt accagaagaa gctggacaac agggagaagg agagggtggc
     4261 agcaaggcag gcctggtctg tggtgggcac aatcaaggat ctgaagcagg gctatctgag
     4321 ccaggtcatc cacgagatcg tggacctgat gatccactac caggccgtgg tggtgctgga
     4381 gaacctgaat ttcggcttta agagcaagag gaccggcatc gccgagaagg ccgtgtacca
     4441 gcagttcgag aagatgctga tcgataagct gaattgcctg gtgctgaagg actatccagc
     4501 agagaaagtg ggaggcgtgc tgaacccata ccagctgaca gaccagttca cctcctttgc
     4561 caagatgggc acccagtctg gcttcctgtt ttacgtgcct gccccatata catctaagat
     4621 cgatcccctg accggcttcg tggacccctt cgtgtggaaa accatcaaga atcacgagag
     4681 ccgcaagcac ttcctggagg gcttcgactt tctgcactac gacgtgaaaa ccggcgactt
     4741 catcctgcac tttaagatga acagaaatct gtccttccag aggggcctgc ccggctttat
     4801 gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag tttgacgcca agggcacccc
     4861 tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat cacagattca ccggcagata
     4921 ccgggacctg tatcctgcca acgagctgat cgccctgctg gaggagaagg gcatcgtgtt
     4981 cagggatggc tccaacatcc tgccaaagct gctggagaat gacgattctc acgccatcga
     5041 caccatggtg gccctgatcc gcagcgtgct gcagatgcgg aactccaatg ccgccacagg
     5101 cgaggactat atcaacagcc ccgtgcgcga tctgaatggc gtgtgcttcg actcccggtt
     5161 tcagaaccca gagtggccca tggacgccga tgccaatggc gcctaccaca tcgccctgaa
     5221 gggccagctg ctgctgaatc acctgaagga gagcaaggat ctgaagctgc agaacggcat
     5281 ctccaatcag gactggctgg cctacatcca ggagctgcgc aacaaaaggc cggcggccac
     5341 gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac ccatacgatg ttccagatta
     5401 cgcttatccc tacgacgtgc ctgattatgc atacccatat gatgtccccg actatgccta
     5461 aagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5521 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     5581 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5641 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5701 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     5761 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5821 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5881 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5941 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     6001 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6061 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6121 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6181 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6301 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6361 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     6421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     6541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6601 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6661 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6721 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6781 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6841 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6901 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6961 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7021 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7081 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7141 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7201 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7261 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7321 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7381 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7441 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7501 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7561 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7621 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7681 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7741 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7801 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7861 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7921 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7981 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8041 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8101 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8161 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8221 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8281 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8341 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8401 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8461 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8521 cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     8581 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     8641 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     8701 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     8761 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     8821 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     8881 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     8941 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     9001 tgtgcagcga tgggggcggg gggggggggg gggsgggccc rgncgggggg gggggggggg
     9061 gggggggggg gggggggggg gggggggggg gggggggggg gggggggngg gggggggggg
     9121 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggg
//
