LOCUS       sequence-259781-       12826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Alpsoy A, Dykhuizen EC
  TITLE     Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its 
            paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes.
  JOURNAL   J Biol Chem. 2018 Jan 26. pii: RA117.001065. doi: 
            10.1074/jbc.RA117.001065.
  PUBMED    29374058
REFERENCE   2  (bases 1 to 12826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2018 Jan 26. pii: RA117.001065. doi: 
            10.1074/jbc.RA117.001065."
COMMENT     Sequence Label: TetO-FUW-BICRAL-FLAG-pgk-puro
FEATURES             Location/Qualifiers
     source          1..12826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(88..587)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(136..155)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     529..552
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(613..636)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3986..4010)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4007..4045)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(4078..4096,4097..4119,4120..4138,4139..4161,4162..4180,
                     4181..4203,4204..4222,4223..4245,4246..4264,4265..4287,
                     4288..4306,4307..4329,4330..4348)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    4405..4522
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4820..4864)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5049..5282
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5775..5900
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5947..6127
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6145..6343)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6345..6724
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6896..6915
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6990..7094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7095..7163,7164..7955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7313..7332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8126..8714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8615..8634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8868..8885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9002..9023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9038..9043,9044..9061,9062..9068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9076..9092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9081..9103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9100..9116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9100..9116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9165..9286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9176..9195)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9230..9249
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9416..9790)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(9809..9856)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(9904..10233)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9918..10053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9972..9991)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     10218..10238
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(10247..10675)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10357..10378)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10569..10588
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    10721..10945
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10934..10951
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             10977..11157
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     11662..11678
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     11663..11679
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11682..12270
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             11788..11799
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     12197..12217
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(12294..12826,1..67))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12377..12397)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
       61 cggtcatggt aagcttgggc tgcaggtcga aaggcccgga gatgaggaag aggagaacag
      121 cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc
      181 gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct
      241 ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg
      301 cttccattgc tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat
      361 ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg
      421 agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga
      481 tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc
      541 taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt aaccggtatg
      601 gaattctcat cacttgtcgt catcgtcttt gtagtctccg ccacactcta ggatactatt
      661 tacagctgct tctaaaatgg agtcagtttc tacaacacct tcactgtggt ccgggatgaa
      721 cttttccagg cagttttcct gtgaagccat aggagaaaag ttggggaaat ctaactcaac
      781 agagccgcta accgtggggt cactctgggg ctgccgtccc gcctttttga gttccaagat
      841 gttcttaaat gtggtttcca agctctttgt cagctggaga tctggctggg gttcgcctga
      901 ccctttcatg atgtctgtgt gtaaaacttc attctttgga gaattcctca gacttttgtc
      961 ctggaaggaa ttgttacacg tcatctccaa gtgcgaatct gctaggatgc ttgacagttt
     1021 gctctcagta ccatgatccg atctggatga ggttttccgg tgcccctcag ggccgggaga
     1081 gcactgactg gccttcagag gctctctccg gctggccttc tcttcagacg tgctctggta
     1141 ctgcactaag cccactttgt caaatttcag tgctctgcca aggcattctg tttttttaga
     1201 aatgtttcct tctgcagaga ccacgatgtg attaggcact agatgaaact ggtcatgatt
     1261 catgggttcc gtcacaccgg ttttggctcg gtctctgcct tgggccttgg ctggcggttg
     1321 cagagagctg cttgctttac tgccatgctg gtcactccgg ccaaactgtg tctcatgagc
     1381 agctttatca agtttgaagg aacaacagaa atcagcctga aaaccctcag ggtctacaag
     1441 tgccaaacgc ttgtctcggg acagggaagc tctttcctcc tggttgaaca tcctatcgat
     1501 catcaccatc tcagcagagg gattgattcg catggcatct tctaggagca ggcatctgta
     1561 tttgttaagc atagcttggg tcctttttag gagctgagtg gcaactgtct caaattcatt
     1621 gtccactttt ctcaagtctt cttcagtggg catggagccc tggcacacgt ggtaggagag
     1681 cagtctctgt accgcatcac ttagtgatcg gaagtgactt ttgtctggtg tcacagtgtg
     1741 ggcttggtcc ctctgcaact gctggagaat gaaagctcct ttcgttagct gtttcgcagc
     1801 aggccttttt tgtcctcccg aatgactctc cacctgcaca gctggatggc caggagatga
     1861 tccaactact ttttcctgct gtggttgtgc ggttcctaag gatgcggata taactttgct
     1921 tccagaatcc tgccctggca gtgtggtgct caagagccca gggatctgtg cttgcctcag
     1981 gccgtctgtg gtcttgggag cagaaatggg ggaagtctga ttcagacatt ttttctgagc
     2041 ctggcagaag aattgttgct gggttcctgt tccaggtgtg ttactgaagg tgctggtaga
     2101 cttggaagaa gaaactggaa gacgatgaga cacactgact ggtactggag tcctgtttgg
     2161 ctgggtcagc tgatctcctg taaagtttga tcctgatgaa ccagactgaa ctgcagaccc
     2221 aagactagga tgggcagtgc tggctgagct gacagcaggg aaccgactag gtcctgacat
     2281 gcttgtcacc gatggcatgg tggcgaagcc aggtcttcct tgggaaacgc tgctctgccc
     2341 aggtgacagg tgtaatacag ttggggatgc ctgctgcaag ggcatctgtc cacccacaag
     2401 ctgaggagag gcatgattgg ctatcactgg acttccagag gcagcaaatg tctgcccaga
     2461 cactaagtgg acagcagtat tctggttgtt aagcatcggt ccagtatatg gttggttggt
     2521 cctgagcatg ttggaagagt ttctgttcaa catgacatgc tctgaggcca cttggccaga
     2581 aatgagctga gagggttgag tttgaagaag ttgcccattt atagtctgga cgtggtgtac
     2641 cgagttggaa ctgacagaaa ggcttgtagg tataaggaac tgactttggg gtgcgtgagg
     2701 ttggcccatg ggggaatgaa taacaatact gcccccagtg ctgctcactg cctgtgaggt
     2761 gactggcttt cttgtgttct gttggtttac aatgttcaca gacatgtgtg gaccaactac
     2821 tgagccctgg ggtgagcttg cagaagcaaa agagatccct tgttgtacgt ggtgctgctg
     2881 aattcccaaa ttgttcacat tgtatgtatt ttgctgaccc atctggatag gctttggctg
     2941 tatattaatt gggactttat ttgaatttgg tgcaagaccc ctttgtatga tgatgttatg
     3001 cacaggtaaa gatgtttgaa aatttgtgct gttaaatgga acggtaacag gctgtgctac
     3061 tggactggaa ctagagttcc caaacaagga gttgccatta ggagtctgtc tatgaaccag
     3121 gagccctcca ctcacatttg atggggcttg ctgcccgctg ccctttaata tgatttgaga
     3181 tccatctagg ttattaatag tcatcatgga aggatgatta ccaaatgacc caattaactg
     3241 tatttgacca gaaccactaa tctgactgct attagacaaa tgctggctgg gaacactgat
     3301 ccccacatgt tgcataaagc catgttgtac acccactgta ttgcttgcaa acgatgctcc
     3361 aacaggcaca tgcgtcaccc ctataggctg cagcgtctga cctgagtaat tagaaacatt
     3421 agaagcctga gtaaaggatg ctgatgaaga aggatgaagc tgagcttgtg caaactgttg
     3481 gcttgaacct atactggcat ccaaatatgc ctcttctgcc aatgtctgtt cagtgatatt
     3541 ggcctcttgc aaggatttct gaagaatgtc gaaaggttgg tcctcagtga gatcaggaag
     3601 aggagaagac tcgagttcat cttcaagaaa ctggaggcta cttgaaagtg gcagtccatc
     3661 actgggccct tctccaagct ggttgcttac acctttgagg gatgacttag gatcagcatt
     3721 actagagttg gcgaaaattg aatttgaatt ggctgcagaa tatcctgcat tagtcaagtc
     3781 atcattgcta gatttattac taggtccatg tagaaaatag ttcaatgctt gtgggtctcc
     3841 aataagatca aggagacacg agtcatcatc atcatccatg gggcgaattc acggatccat
     3901 cggctagcaa ttcggggccg cggaggctgg atcggtcccg gtgtcttcta tggaggtcaa
     3961 aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct ctgcttatat
     4021 aggcctccca ccgtacacgc ctacctcgac ccgggtaccg agctcgactt tcacttttct
     4081 ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt
     4141 ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac
     4201 ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata
     4261 gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac
     4321 tttcactttt ctctatcact gatagggagt ggtaaactcg agatctaacc aaactggatc
     4381 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
     4441 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
     4501 aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtctgc agaattggcg
     4561 cacgcagtgc cgatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
     4621 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
     4681 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     4741 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     4801 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     4861 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
     4921 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
     4981 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
     5041 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
     5101 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
     5161 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
     5221 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
     5281 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
     5341 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
     5401 tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt
     5461 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     5521 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     5581 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     5641 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     5701 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     5761 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     5821 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     5881 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     5941 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     6001 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     6061 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     6121 gagacccagt acaggcaaaa cgcgctgctt atatagacct cccaccgtac acgcctaccg
     6181 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
     6241 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
     6301 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
     6361 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     6421 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     6481 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     6541 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     6601 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     6661 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     6721 tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc
     6781 agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg
     6841 cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa ctatgcggca
     6901 tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc gatccgtcga
     6961 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7801 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8761 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8821 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8881 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8941 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9001 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9061 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9121 attacgccaa gctctagcta gaggtcgacg gtatacagac atgataagat acattgatga
     9181 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     9241 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttgggg tgggcgaaga
     9301 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     9361 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtagc acgtgtcagt
     9421 cctgctcctc ggccacgaag tgcacgcagt tgccggccgg gtcgcgcagg gcgaactccc
     9481 gcccccacgg ctgctcgccg atctcggtca tggccggccc ggaggcgtcc cggaagttcg
     9541 tggacacgac ctccgaccac tcggcgtaca gctcgtccag gccgcgcacc cacacccagg
     9601 ccagggtgtt gtccggcacc acctggtcct ggaccgcgct gatgaacagg gtcacgtcgt
     9661 cccggaccac accggcgaag tcgtcctcca cgaagtcccg ggagaacccg agccggtcgg
     9721 tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag caccggaacg gcactggtca
     9781 acttggccat ggtttagttc ctcaccttgt cgtattatac tatgccgata tactatgccg
     9841 atgattaatt gtcaacacgt gctgatcaga tccgaaaatg gatatacaag ctcccgggag
     9901 ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     9961 ggcagaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
    10021 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
    10081 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
    10141 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
    10201 gcctggggac tttccacacc ctaactgaca cacattccac agaattaatt cgcgttaaat
    10261 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
    10321 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
    10381 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
    10441 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
    10501 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
    10561 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
    10621 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga
    10681 taccccctag agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc
    10741 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
    10801 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
    10861 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
    10921 gatggctggc aactagaagg cacagtcgag gctgatcagc gggtttaaac gggccctgct
    10981 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa
    11041 cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg
    11101 gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt
    11161 acagtccgga tgcagctctc gggccatgtg atgaaatgct aggcggctgt caaacctcca
    11221 ctctaatact tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagcg
    11281 ggtgttctct ccttcattgg cttcttctac cttctcttgc tcaactggta ctagcttgta
    11341 gcaccatcca aaggtcagtg gatatctgat ccctggccct ggtgtgtagt tctgccaatc
    11401 agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttcgttggga
    11461 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
    11521 cttgtaagtc attggtctta aaggtacctg aggtgtgact ggaaaaccca cctcctcctc
    11581 ctcttgtgct tctagccagg cacaatcagc attggtagct gctgtattgc tacttgtgat
    11641 tgctccatgt ttttctaggt ctcgaggtcg acggtatcga tgcggggagg cggcccaaag
    11701 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
    11761 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
    11821 cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc
    11881 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
    11941 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
    12001 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
    12061 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
    12121 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
    12181 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
    12241 ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga atttcaggca
    12301 ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg
    12361 acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag
    12421 gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga
    12481 cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg
    12541 ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc
    12601 aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc
    12661 gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc
    12721 ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga
    12781 tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcga
//
