LOCUS       sequence-25981-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Zeng Y, Cullen BR
  TITLE     Sequence requirements for micro RNA processing and function in human
            cells.
  JOURNAL   RNA. 2003 Jan . 9(1):112-23.
  PUBMED    12554881
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2003 
            Jan . 9(1):112-23."
COMMENT     Sequence Label: pCMV-miR21
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(487..505)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        573..677
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcgttt agtgaagtca agcttaagtg accagctaca gtcggaaacc atcagcaaga
       61 ggtcattgtt caccatgggt taacggatcc atcgattcta gaggtaccgc tagcctcgag
      121 atctgtcggg tagcttatca gactgatgtt gactgttgaa tctcatggca acaccagtcg
      181 atgggctgtc tgacaggatc ctcgagtgac cttcagacct tggcactgga ggtggcccgg
      241 cagaagcgcg gcatcgtgga tcagtgctgc accagcatct gctctctcta ccaactggag
      301 aactactgca actaggccca ccactaccct gtccacccct ctgcaatgaa taaaaccttt
      361 gaaagagcac tacaagttgt gtgtacatgc gtgcatgtgc atatgtggtg cggggggaac
      421 atgagtgggg ctggctggag tggcgatgat aagctgtcaa acatgagaat tcttgaagac
      481 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      541 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      601 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      661 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      721 cggcattttg ccnnnctgtt tttgctcacc cagaaacgct ggtgaaagta aanannntga
      781 anatcanttg ggngcacga
//
