LOCUS       sequence-25982-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Ortiz-Martin I, Macho AP, Lambersten L, Ramos C, Beuzon CR
  TITLE     Suicide vectors for antibiotic marker exchange and rapid generation 
            of multiple knockout mutants by allelic exchange in Gram-negative 
            bacteria.
  JOURNAL   J Microbiol Methods. 2006 Dec . 67(3):395-407.
  PUBMED    16750581
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2006 Dec . 67(3):395-407."
COMMENT     Sequence Label: pIOM17
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     191..210
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cccccccgcc gctgtctcat gcattgggaa tatcaacggt ggtatatcca gtgatttttt
       61 tctccatttt agcttcctta gctcctgaaa acctcgccaa gctagcttgg attctcacca
      121 ataaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagactcgag caagacgttt
      181 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
      241 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
      301 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc
      361 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct
      421 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc
      481 aggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc tattctgacc
      541 ttgccatcac gactgtgctg gtcattaaac gcgtattcag gctgaccctg cgcgctgcgc
      601 agggctttat tgattccatt tttacactga atgaatgttc cgttgcccct gcccgggcta
      661 cagagcttgg gggtaccgag ctgcgaattc gcggcgtgcc cgccacaacg ctgcctaggg
      721 cgtgttaata ttccggtagt atacctagcc tgcctaacgt cgaacaaccg cggtctcttc
      781 tagaactata agctaggcat gctcaacatt taaaggcgct gatcgtccaa acgcccggcg
      841 gatcctcgcc gtggatgatc attgcaagtt ggtatctcat tatattaggg gaccccgggt
      901 tgatattata tcttcagggt tcgaagaaat ggaaaacccc cgtaac
//
