LOCUS       sequence-259829-        3654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor 
            cloning), Terminator: pea rbcsE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C2906
FEATURES             Location/Qualifiers
     source          1..3654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2483..2587
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct actaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgctcacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccacc
      781 tgcaacgccg gcgtaatatg gcgcgccagc tggcttgtgg ggaccagaca aaaaaggaat
      841 ggtgcagaat tgttaggcgc acctaccaaa agcatctttg cctttattgc aaagataaag
      901 cagattcctc tagtacaagt ggggaacaaa ataacgtgga aaagagctgt cctgacagcc
      961 cactcactaa tgcgtatgac gaacgcagtg acgaccacaa aacagctggc ttgtggggac
     1021 cagacaaaaa aggaatggtg cagaattgtt aggcgcacct accaaaagca tctttgcctt
     1081 tattgcaaag ataaagcaga ttcctctagt acaagtgggg aacaaaataa cgtggaaaag
     1141 agctgtcctg acagcccact cactaatgcg tatgacgaac gcagtgacga ccacaaaaga
     1201 attccctcta tataagaagg cattcattcc catttgaagg atcatcagat actgaaccaa
     1261 tatttctcac tctaagaaat taagagcttt gtattcttca atgagaggct aagacctgca
     1321 ggatgtctga gccacctcgg gctgagacct ttgtattcct ggacctagaa gccactgggc
     1381 tcccaaacat ggaccctgag attgcagaga tatccctttt tgctgttcac cgctcttccc
     1441 tggagaaccc agaacgggat gattctggtt ccttggtgct gccccgtgtt ctggacaagc
     1501 tcacactgtg catgtgcccg gagcgcccct ttactgccaa ggccagtgag attactggtt
     1561 tgagcagcga aagcctgatg cactgcggga aggctggttt caatggcgct gtggtaagga
     1621 cactgcaggg cttcctaagc cgccaggagg gccccatctg ccttgtggcc cacaatggct
     1681 tcgattatga cttcccactg ctgtgcacgg agctacaacg tctgggtgcc catctgcccc
     1741 aagacactgt ctgcctggac acactgcctg cattgcgggg cctggaccgt gctcacagcc
     1801 acggcaccag ggctcaaggc cgcaaaagct acagcctggc cagtctcttc caccgctact
     1861 tccaggctga acccagtgct gcccattcag cagaaggtga tgtgcacacc ctgcttctga
     1921 tcttcctgca tcgtgctcct gagctgctcg cctgggcaga tgagcaggcc cgcagctggg
     1981 ctcatattga gcccatgtac gtgccacctg atggtccaag cctcgaagcc tgataactcg
     2041 aggttcgagt attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta
     2101 atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga
     2161 agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc
     2221 tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag
     2281 taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt
     2341 gtagttgtga gctcgcgcgt agtcctcggt aatatcgcag aacaaaagta cctgatatcg
     2401 agtgtacttc aagtcacacc ggcgagtgac tagcaggtga tgaaaagcct tagggtcagg
     2461 tggcactttt cggggaaatg tgcgatgaac ccctatttgt ttatttttct aaatacattc
     2521 aaatatgtat ccgctaatga gacaataacc ctgataaatg cttcaattat attgaaaaag
     2581 gaagagtatg agtatccaac atttccgtgt cgcccttatt cccttttttg cggccttctg
     2641 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2701 gggtgcccga gtgggttaca ttgaactgga tctcaacagc ggcaaaatct tagagagttt
     2761 tcgccccgaa gaacgcttcc caatgatgag cactttcaaa gttctgctat gtggcgcggt
     2821 gttatcccgt attgatgctg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2881 tgacttggtt gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2941 agaattatgt agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3001 aacgattgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3061 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3121 cacgatgcct gtagcaatgg caacaactct ccgcaaacta ttaactggcg aactacttac
     3181 tcttgcttca cgccagcaac tcattgactg gatggaggcg gacaaagttg caggaccact
     3241 tctgcgctcg gcacttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     3301 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     3361 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     3421 aggtgcctca ctgattaagc attggtaact gtcagacgaa gggaaataaa tagtagcccg
     3481 cctgatgtgc gggctttttt ttggtggtgg ttaccagtgt cgcttagcta ccgtaggtcc
     3541 tcttaggcgg tccgattagc gacatagtca tcaatgcctt gacggcaaaa tcccttaacg
     3601 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
