LOCUS       sequence-25983-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Ortiz-Martin I, Macho AP, Lambersten L, Ramos C, Beuzon CR
  TITLE     Suicide vectors for antibiotic marker exchange and rapid generation 
            of multiple knockout mutants by allelic exchange in Gram-negative 
            bacteria.
  JOURNAL   J Microbiol Methods. 2006 Dec . 67(3):395-407.
  PUBMED    16750581
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2006 Dec . 67(3):395-407."
COMMENT     Sequence Label: pIOM17
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(202..306)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggttcttc ggggcgaaaa ctctcaaggt atcttaccgc atgattgaga tccagnttcg
       61 ntgttnaccc acgtcgntgc acccaactgc atcnttcagc atcttttacg tttcaccagc
      121 gntttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      301 tccgcgcaca tttccccgaa aagtgccacc tgcagatcca tgggacgtcn acgtttggcc
      361 ttacttaacg gagaaccatt aagccttagg acgcttcacg ccatacttgg aacgagcctg
      421 cttacggtct ttaacgccgg agcantcaag cgcaccacgt acggtgtggt aacgaacacc
      481 cgggaggtct ttaacacgac cgccacggat caggatcacg gagtgctcct gcaggttgtg
      541 accntcacca ccgatgtagg aagtcacttc taaacngtta gtcannacna acacggcata
      601 cctttncgca gcgcggagat cgggtttttt aggagtggta gnatatanan gagtacntac
      661 nccacgnttt tgcgggcatg ctttccngcg caggcacgtt tnctttcgca actttgcnng
      721 cangnagntt ngcnnacnac tggttaactg ttgccattan anaccnccag gattangctt
      781 ttnttcntaa ntgntnatta actgtcncnc ncanatgagg cnccgaantn aaggcgannc
      841 cnaacag
//
