LOCUS       sequence-259834-        3232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG inducible expression of S-TAGA-Evo1 qtRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   DeBenedictis EA, Carver GD, Chung CZ, Soll D, Badran AH
  TITLE     Multiplex suppression of four quadruplet codons via tRNA directed 
            evolution.
  JOURNAL   Nat Commun. 2021 Sep 29;12(1):5706. doi: 10.1038/s41467-021-25948-y.
  PUBMED    34588441
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-29"; volume: "12(1):5706. doi"; pages: " 
            10.1038/s41467-021-25948-y"
COMMENT     Sequence Label: pED14xS-11
FEATURES             Location/Qualifiers
     source          1..3232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    252..268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1347..1438)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(1448..2596)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     promoter        complement(2623..2657)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
ORIGIN
        1 aagaggacat ccggtcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
       61 gctgaggaga cttagggacc cttgtgcttc tcaaatgcct gaggccagtt tgctcaggct
      121 ctccccgtgg aggtaataat tgacgatatg atcagtgcac ggctaactaa gcggcctgct
      181 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
      241 cagtaagata attgtgagcg gataacaatt ggagagatgc cggagcggct gaacggaccg
      301 gtattctaac accggagtag gggcaactct accgggggtt caaatccccc tctctccgcc
      361 actttatcaa tgacttatct cccgacttcc caggatccgt cgacctgcag atccttagcg
      421 aaagctaagg atttttttta agcttctgcc attcatccga cttaattaac ggcactcctc
      481 agcaaatata atgaccctct tgataaccca agagggcatt ttttaatgcc catggcgttt
      541 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat
      601 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta
      661 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg
      721 gcgcgatttt gcccaccaag gtagtcggcg gttactgcgc tgtaccaaat gcgggacaac
      781 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
      841 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
      901 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
      961 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
     1021 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
     1081 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
     1141 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
     1201 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
     1261 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
     1321 acttcggcga tcaccgcttc cctcatactc ttcctttttc aatattattg aagcatttat
     1381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taggccaaat
     1441 aggccgtcta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
     1501 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
     1561 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
     1621 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
     1681 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
     1741 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
     1801 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
     1861 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
     1921 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
     1981 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
     2041 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
     2101 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
     2161 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
     2221 tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg
     2281 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
     2341 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
     2401 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca
     2461 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
     2521 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
     2581 caaacgtccg attcattcac cctccttata tcgtatgggg ctgctagcat aatacctagg
     2641 gctgagctag ccgtaaagtg aaagaagaga aacctccatt tttggggtga ggccgttcgc
     2701 ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc
     2761 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt
     2821 taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc
     2881 cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa
     2941 atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg
     3001 cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca
     3061 ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg
     3121 aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg
     3181 cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gt
//
