LOCUS       sequence-25987-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Ortiz-Martin I, Macho AP, Lambersten L, Ramos C, Beuzon CR
  TITLE     Suicide vectors for antibiotic marker exchange and rapid generation 
            of multiple knockout mutants by allelic exchange in Gram-negative 
            bacteria.
  JOURNAL   J Microbiol Methods. 2006 Dec . 67(3):395-407.
  PUBMED    16750581
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2006 Dec . 67(3):395-407."
COMMENT     Sequence Label: pIOM15
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(221..325)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tccggnnggn atagccanat gcgaaacgtt tcttcggggc gaaaactctc aaggatctta
       61 ccgctgnttg agatccagnt tcgnngttta cccacgtcgn tgcacccaac tgatcttcag
      121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
      181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
      241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
      301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcagatccat
      361 gggacgtcga cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc
      421 catacttgga acgagcctgc ttacggtctt taacgccgga gcagtcaagc gcaccacgta
      481 cggtgtggta acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg
      541 agtgctcctg caggttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag
      601 tcagacgaac acggcatact ttacgcagcg cggagttcgg tttttttagg agtggtagta
      661 tataccacga gttgatacgc cacgtttttn gcggggcatg cctccagcgc angcacgttg
      721 cttttccgca actttgcgac acgtnggttt ngcgtaccag ctggttaact ggtgccatcc
      781 anagctcctg gttttagctt tgcttcntaa acacgtaana
//
