LOCUS       sequence-25991-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Zeng Y, Wagner EJ, Cullen BR
  TITLE     Both natural and designed micro RNAs can inhibit the expression of 
            cognate mRNAs when expressed in human cells.
  JOURNAL   Mol Cell. 2002 Jun . 9(6):1327-33.
  PUBMED    12086629
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2002 Jun . 9(6):1327-33."
COMMENT     Sequence Label: pCMV-miR30
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           154..168
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(487..505)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        573..677
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcgttt agtgaagtca agcttaagtg accagctaca gtcggaaacc atcagcaaga
       61 ggtcattgtt caccatgggt taacggatcc atcgattcta gaggtaccgc tagcctcgag
      121 atctgcgact gtaaacatcc tcgactggaa gctgtgaagc cacagatggg ctttcagtcg
      181 gatgtttgca gctgcggatc ctcgagtgac cttcagacct tggcactgga ggtggcccgg
      241 cagaagcgcg gcatcgtgga tcagtgctgc accagcatct gctctctcta ccaactggag
      301 aactactgca actaggccca ccactaccct gtccacccct ctgcaatgaa taaaaccttt
      361 gaaagagcac tacaagttgt gtgtacatgc gtgcatgtgc atatgtggtg cggggggaac
      421 atgagtgggg ctggctggag tggcgatgat aagctgtcaa acatgagaat tcttgaagac
      481 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      541 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      601 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      661 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatn ncctttttgc
      721 ggcattttgn cttnctgttt ttgntcncca gaancgctgn naaagtaaan a
//
