LOCUS       sequence-25993-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Ortiz-Martin I, Macho AP, Lambersten L, Ramos C, Beuzon CR
  TITLE     Suicide vectors for antibiotic marker exchange and rapid generation 
            of multiple knockout mutants by allelic exchange in Gram-negative 
            bacteria.
  JOURNAL   J Microbiol Methods. 2006 Dec . 67(3):395-407.
  PUBMED    16750581
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2006 Dec . 67(3):395-407."
COMMENT     Sequence Label: pIOM14
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(203..307)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atattgggaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
       61 gttcgatgtt aacccactcg atgcacccaa ctgatcttca gcatcgtttt actttcacca
      121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
      181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
      241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      301 ttccgcgcac atttccccga aaagtgccac ctgcagatcc atgggacgtc gacgtttggc
      361 cttacttaac ggagaaccat taagccttag gacgcttcac gccatacttg gaacgagcct
      421 gcttacggtc tttaacgccg gagcagtcaa gcgcaccacg tacggtgtgg taacgaacac
      481 ccgggaggtc tttaacacga ccgccacgga tcaggatcac ggagtgctcc tgcaggttgt
      541 gaccttcacc accgatgtag gaagtcactt cgaaaccgtt agtcagacga acacggcata
      601 ctttacgcag cgcggagttc ggttttttag gagtggtagt atatacacga gtacatacgc
      661 cacgtttttg cgggcatnct tccagcgcag gcacgttgct tttcgcanct ttgcgagcac
      721 gtggtttgcg taccanctgg ttaactgttg ccattaaata gctcctggtt ttagctttgc
      781 ttcgtacncc ngtataaaac gtccnncaca tatgaggang ccgaatttna g
//
