LOCUS       sequence-259973-       11430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 
            locus in place of a STOP codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Kaczmarczyk L, Reichenbach N, Blank N, Jonson M, Dittrich L, Petzold
            GC, Jackson WS
  TITLE     Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and 
            augment a growing collection of Cre drivers and effectors in the 
            129S4 genetic background.
  JOURNAL   Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.1038/s41598-021-84887-2.
  PUBMED    33686166
REFERENCE   2  (bases 1 to 11430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-03-8"; volume: "11(1):5412. doi"; pages: " 
            10.1038/s41598-021-84887-2"
COMMENT     Sequence Label: Slc1a3-CreERT2 Targeting Vector
FEATURES             Location/Qualifiers
     source          1..11430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2166..2186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3307..3363
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3364..4392
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             4405..5340
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4453..5346
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     protein_bind    5365..5398
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        5460..5959
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        5971..6018
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6037..6840
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6100..6119)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6710..6729
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6872..6889)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6878..7102
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7164..7197
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        8854..9353
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     regulatory      9365..9374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    10612..10733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10649..10668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10703..10722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10783..10802)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10784..10802)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10812..10829)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10812..10828)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10821..10843)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10970..11425
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11057..11076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11267..11288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gctcccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agcgcgcaat taaccctcac taaagggaac aaaagctggg taccggcgcg cctttttatc
     2221 ccagcacgct tctccaagga gactctgtcc catgctttga gaactgttag tacaatggaa
     2281 ggtgaatgaa agaaagagaa aatgttaaat aaactcagga aagaggactg gggaaatgac
     2341 tcatttggta tggttgcatg atgtgtgcat gagggcttga gttccagcct cggaacagtg
     2401 cccatgtaaa aagagcagag cacaatgtca cacacctata aatctccaac attggcactg
     2461 gaaaggcaga gggaagcaag aggacccttt ggaacggcta gcaagccaat ctagctaaat
     2521 ctccagtgag acaccttatt tcaaaacaaa gtggagaata attaaagacc acacttctgg
     2581 cttccacaca tatgtacatg cacacataga gcgtgtgcgg gtgctcgagc acacacacac
     2641 acatgggggg ggggggggag gtcctcactg ttcaccttct gtttatagtc acactaaata
     2701 gctggaaact aatcacttac ttgtgattag ttgtaggtgt tgtgggtcca agtatgaaat
     2761 cattccacta ttacattacc cagaacttaa aagatacttc tttttttttt aagatttatt
     2821 tatttattat atgtaagtac actgtagctg tcttcagaca ctccagaaga gggcgtcaga
     2881 tctcattaca gatggttatg aggcaccatg tggttgctgg gaattgaact caggacctct
     2941 agaagagcag tcagtgctct taaccactga gccatctctc cagcccctaa aagatccttc
     3001 taaagccttc tccagccaac tcacgatagg tgctgggatc tagtggtttt catgggaccc
     3061 tctcttttcc ttcctgcctg cctcacctgc agggaccgcc tccgaaccac caccaacgta
     3121 ctgggtgact ccctcggagc agggattgtc gagcacttgt cccgacatga actgaagaac
     3181 cgagatgttg aaatggggaa ctcggtgatt gaggagaacg aaatgaagaa gccgtatcag
     3241 ctgattgccc aggacaatga accggagaaa cccgtggcag acagcgaaac caagatggga
     3301 tccggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
     3361 cctatggcca atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg
     3421 agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag
     3481 catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat
     3541 aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag
     3601 gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa catgcttcat
     3661 cgtcggtccg ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg
     3721 atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc
     3781 actgatttcg accaggttcg ttcactcatg gaaaatagcg atcgctgcca ggatatacgt
     3841 aatctggcat ttctggggat tgcttataac accctgttac gtatagccga aattgccagg
     3901 atcagggtta aagatatctc acgtactgac ggtgggagaa tgttaatcca tattggcaga
     3961 acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa
     4021 ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa ctacctgttt
     4081 tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct atcaactcgc
     4141 gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa ggatgactct
     4201 ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg
     4261 gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat
     4321 attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg
     4381 gaagatggcg atctcgagcc atctgctgga gacatgagag ctgccaacct ttggccaagc
     4441 ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag
     4501 atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta tgatcctacc
     4561 agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg
     4621 gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat
     4681 caggtccacc ttctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc
     4741 tccatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga caggaaccag
     4801 ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac atcatctcgg
     4861 ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat tattttgctt
     4921 aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga gaaggaccat
     4981 atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc caaggcaggc
     5041 ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct ctcccacatc
     5101 aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa gaacgtggtg
     5161 cccctctatg acctgctgct ggaggcggcg gacgcccacc gcctacatgc gcccactagc
     5221 cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc gggctctact
     5281 tcatcgcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccaca
     5341 gcttgaacgg acagagggcc ctaagaagtt cctattctct agaaagtata ggaacttcgt
     5401 cgacatttct aaatttaaat cttaaggcta gcttggctgc aggtcgtcga aattctaccg
     5461 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5521 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5581 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5641 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5701 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5761 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5821 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5881 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5941 ctcatctccg ggcctttcga cctgcagcct gttgacaatt aatcatcggc atagtatatc
     6001 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg gatcggccat tgaacaagat
     6061 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6121 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6181 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     6241 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6301 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6361 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6421 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6481 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6541 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc
     6601 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6661 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6721 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6781 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6841 ggggatcaat tctctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     6901 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6961 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     7021 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     7081 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgactaga
     7141 gcttgcggaa cccttcgaat ttcgaagttc ctattctcta gaaagtatag gaacttcaag
     7201 tttaaacatg tagacggaca gagaagtgct ttcttaagca ccaagtgttg gaaactgttc
     7261 tacaatgtat ccatctccct gggctcactt tcccagtgag ctcctctttc ctccctactc
     7321 tgataggatt ggaaaacggc caaacacaaa ggagggctct gcagcagccc aaacctatcg
     7381 gttttagcct acatttgaaa attttaagtc atttcatatt attcttacca agtaagctac
     7441 tacaatcaaa cacagtttcg atattaccaa tttaggtggc aaacgatcct gtgccattgc
     7501 tctgtaagta aaagaattaa gcaaatgata ggctaccgaa aaaaaaagtt ttaaaatcaa
     7561 ctttcaagat gtaaaaatcc ttcagaatgc aactgagttt taatcttaaa acattacaac
     7621 ttgatgagca attatcagtt acccattgag tagctgagag gttcccattt cttctgactc
     7681 ctgccctgac tttgtcagca tcagggcaat ccccacatgc acacagcaca gctgtgagga
     7741 ggagcggaga gtgaggctcc caaatggtct agagtgagcc ttgtctcatc cattggcctc
     7801 agtgttctca tccggaaaat gagtgactcc tagacaggcc tctctagccc aaacttctgg
     7861 aacatcagga aggaccctgc ccctgtacac catgaggatc tcagacagga cacttattgg
     7921 gaatggacat ttctctctag gggcaggctg tgtgtggctc actagatcct gggttcaaaa
     7981 tgttcgttct atcagtagac aggtggaagg ccatcctaga aggcctattg tcctgttttg
     8041 ttttccaata ctagaggccc tcccttctgg ggtacacttc ccaaatccaa gggaagacag
     8101 tctcttcaat tagatgtcta ggaaataaag agaagactag caagtgtgct caagtcttag
     8161 tgtggaacac taaagagtgg agaaagaata gagtatggcc aacattacaa aagggaggat
     8221 tctttgaaag atcaggaaaa gttggttttc tttaaagact aaaatggaga gctatatctc
     8281 aacccttttt ctgttagcct aaacacttcc tcttcagtcc ctaatcagcc cccaaagaaa
     8341 cgacgtcata acaccacttc ctcctcatct cccagtccaa ccaaccacat ctctgggaca
     8401 gatccatacg ctgggtggat ggtctcattt ctgaaaaaga tttttttttt ttggtttttt
     8461 tatttgtttt gtttttacta ttttaaccag gcgttcttag gatgacaaaa acaacatact
     8521 cttttttgta tagagatgtc tcttgacaaa tcattcaaaa gtctcatcta gagtggagtg
     8581 tgcttgttta tgtccctacc aaaaaaaaaa aaaaaaaaaa gacagatgaa tcagcatgag
     8641 gataacatca ctgaggatgg aagaaaacac agcaggagaa ttcttttcag gcgaggcagc
     8701 tcccaggttt aaaagagatt atattcactc ccttaggaat atccaatcaa agatactcag
     8761 tgagagccat ctgacagtac cagctgcttc agactttctg agcgaaaatg atgaatggcc
     8821 ggccacccgg gtcctaggac gcgtaattct accgggtagg ggaggcgctt ttcccaaggc
     8881 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     8941 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     9001 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     9061 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     9121 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     9181 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     9241 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     9301 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     9361 ggtagccacc atggcttctt acccttgcca ccagcatgct tccgcattcg atcaagccgc
     9421 tcggagccgc ggacacaata acaggagaac cgcattgcga cctcggcgcc agcagaaagc
     9481 taccgaggtg agactggagc agaagatgcc tacgctcctg agagtgtaca tcgacggccc
     9541 ccatggcatg ggcaagacta ctaccactca gctgctggtc gcattgggga gcagagacga
     9601 cattgtgtat gttccggagc ccatgacata ctggagggtg cttggggcta gtgagaccat
     9661 cgcaaacatc tatactaccc aacatcggct cgatcagggg gaaattagcg ctggagatgc
     9721 cgccgtggta atgaccagtg ctcagattac aatgggaatg ccgtacgctg taacagacgc
     9781 tgtgctggcc ccccatattg gcggtgaagc cgggagcagc cacgcccctc cgccagctct
     9841 gaccctcatc tttgatcggc atcctattgc tgccctcctc tgctacccag cagctcgcta
     9901 cttgatgggg tctatgaccc cccaggccgt tcttgcattc gtcgccctga ttccccccac
     9961 cctgcccggt acgaacatcg ttctgggcgc tctgcctgag gaccggcaca tcgaccgcct
    10021 tgcaaagcga cagagacctg gcgagagact ggatctggca atgctggctg ccatcaggcg
    10081 agtgtacggt ctcctcgcca acaccgtgag atacttgcaa ggtgggggca gctggcggga
    10141 agactggggc cagctgtccg gagcagcagt gccgccccag ggagctgagc ctcagtcaaa
    10201 tgctggacct agaccccata tcggggacac tctgttcact ctctttagag cccctgaact
    10261 cctggcaccg aatggcgatc tgtataacgt tttcgcctgg gctctcgacg tgctggccaa
    10321 gaggcttaga cccatgcacg tcttcatttt ggattacgac cagtccccag cagggtgtag
    10381 ggacgctctg ctccagctga ccagcggtat ggtgcaaaca catgttacta cccctggctc
    10441 cataccaacc atctgtgatc ttgcccgcac atttgcccgc gaaatgggag aggccaactg
    10501 ataaggatcc taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
    10561 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    10621 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10681 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacaacct
    10741 aggacgcgta gcggccgctc accgcggtgg agctccaatt cgccctatag tgagtcgtat
    10801 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    10861 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    10921 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
    10981 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    11041 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    11101 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    11161 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    11221 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    11281 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    11341 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    11401 aacaaaatat taacgcttac aatttaggtg
//
