LOCUS       sequence-259976-        9103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Kaczmarczyk L, Bansal V, Rajput A, Rahman RU, Krzyzak W, Degen J, 
            Poll S, Fuhrmann M, Bonn S, Jackson WS
  TITLE     Tagger-A Swiss army knife for multiomics to dissect cell 
            type-specific mechanisms of gene expression in mice.
  JOURNAL   PLoS Biol. 2019 Aug 8;17(8):e3000374. doi: 
            10.1371/journal.pbio.3000374. eCollection 2019 Aug.
  PUBMED    31393866
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2019-08-8"; volume: "17(8):e3000374. doi"; pages: " 
            10.1371/journal.pbio.3000374. eCollection 2019 Aug"
COMMENT     Sequence Label: CMV-Tagger
FEATURES             Location/Qualifiers
     source          1..9103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             437..526
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             548..604
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1352..1408
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1415..2110
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             2126..2146
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2150..2170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2174..2194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2213..2269
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2279..2344
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2354..2395
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2402..2443
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2450..2491
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      4430..4439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5131..5150)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5132..5150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5155..5289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5179..5198)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5233..5252
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5402..5422)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5402..5420)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5441..5457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5441..5457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5454..5476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5465..5481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5489..5495,5496..5513,5514..5519))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5534..5555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5672..5689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5843..6431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5923..5942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6602..7393,7394..7462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7225..7244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7463..7567)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7593..8048)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7730..7751)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7942..7961
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8119..9103
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag ctacgcgcca attgccacca tggcgcctgt
       61 gaaaaagctt gtggcgaagg ggggcaaaaa aaagaagcag gttttgaagt tcaccctgga
      121 ctgcactcac cctgtagaag atggaatcat ggacgctgcc aattttgagc agttcctcca
      181 ggagagaatc aaggtgaatg ggaaagctgg caacctcggc ggaggagtcg tgaccatcga
      241 acgcagcaag agcaagatca ctgtcacttc agaggtgcct ttctccaaaa ggtatttgaa
      301 atatctcacc aaaaaatatt tgaagaagaa caacctccga gactggctgc gtgttgtcgc
      361 caacagcaaa gagagttacg agctgcgtta cttccagatt aaccaggatg aagaggagga
      421 ggaagacgag gatctatacc catacgatgt tcctgactat gcgggctatc cctatgacgt
      481 cccggactat gcaggatctt atccatatga cgttccagat tacgctgctg atggatccgg
      541 cagcggcgcc acaaacttct ccctgctgaa gcaggccggc gacgtggaag agaatcctgg
      601 cccaatggcg caggtcccag cgagcggaaa gctccttgtc gatccccgat attcgacaaa
      661 cgaccaggaa gaaagcattc tccaggacat catcacgagg tttcccaatg tggtgctcat
      721 gaagcagacg gctcagcttc gagcgatgat gaccatcatt cgtgataaag aaacaccgaa
      781 ggaagaattc gtcttctacg ccgaccgcct gattcgcctc ctcatcgaag aagctttgaa
      841 cgaactgccg ttcgaaaaga aggaagtgac aacccctctg gatgtgtcat accatggagt
      901 ttccttctat tccaagatct gtggcgtctc gattgtgaga gctggcgagt cgatggaaag
      961 cggcttgcgg gcagtttgcc gcggctgccg catcgggaaa atcctcatcc agagagacga
     1021 aacaactgcg gagcctaagc tgatctacga gaagctgcct gccgacatta gagatcgctg
     1081 ggtgatgctg ctagatccga tgtgcgcgac ggcgggcagt gtgtgcaaag cgatcgaggt
     1141 cctcctgagg ctcggcgtga aggaagagag aatcattttc gtcaacattt tggctgctcc
     1201 ccaaggcatt gaacgtgttt tcaaggaata cccgaaagtc cgcatggtca ctgctgctgt
     1261 tgacatctgc ctgaactcga ggtactacat cgtccccggc attggtgatt tcggtgatcg
     1321 gtactttgga accatgggct ccggaagcgg agctactaac ttcagcctgc tgaagcaggc
     1381 tggagacgtg gaggagaacc ctggaccttc cggaatggtg agcgagctga ttaaggagaa
     1441 catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
     1501 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg cggtcgaggg
     1561 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac
     1621 cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc ccgagggctt
     1681 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     1741 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc
     1801 caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg agaccctgta
     1861 ccccgctgac ggcggcctgg aaggcagagc cgacatggcc ctgaagctcg tgggcggggg
     1921 ccacctgatc tgcaacttga agaccacata cagatccaag aaacccgcta agaacctcaa
     1981 gatgcccggc gtctactatg tggacagaag actggaaaga atcaaggagg ccgacaaaga
     2041 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
     2101 ggggcacaga ggtggaagcg gagatcctaa aaagaagaga aaggtggacc ctaagaaaaa
     2161 gagaaaagtg gacccaaaga agaaaagaaa ggtgtctgga tccggctctg gagccaccaa
     2221 cttttctctg ctgaaacagg ccggagatgt ggaggaaaac ccaggccctg gaaccggtga
     2281 ctacaaggac cacgacggag attacaaaga tcacgacatc gattacaagg atgacgatga
     2341 caagtcggcc gccggaaagc ctatcccaaa ccctctcctc ggcctcgatt ctaccggatc
     2401 tggcaagcca atccctaacc cactcctcgg actcgactcc acaggctccg gcaaacccat
     2461 ccctaatcct ctcctcgggc tcgacagcac cggctctggc cgcatgtact cgggagccgg
     2521 ccccgcactt gcacctcctg cgccgccgcc ccccatccaa ggatatgcct tcaagcctcc
     2581 acctagaccc gactttggga cctccgggag aacaatcaaa ttacaggcca atttcttcga
     2641 aatggacatc cccaaaattg acatctatca ttatgaattg gatatcaagc cagagaagtg
     2701 cccgaggaga gttaacaggg aaatcgtgga acacatggtc cagcacttta aaacacagat
     2761 ctttggggat cggaagcccg tgtttgacgg caggaagaat ctatacacag ccatgcccct
     2821 tccgattggg agggacaagg tggagctgga ggtcacgctg ccaggagaag gcaaggatcg
     2881 catcttcaag gtgtccatca agtgggtgtc ctgcgtgagc ttgcaggcgt tacacgatgc
     2941 actttcaggg cggctgccca gcgtcccttt tgagacgatc caggccctgg acgtggtcat
     3001 gaggcacttg ccatccatga ggtacacccc cgtgggccgc tccttcttca ccgcgtccga
     3061 aggctgctct aaccctcttg gcgggggccg agaagtgtgg tttggcttcc atcagtccgt
     3121 ccggccttct ctctggaaaa tgatgctgaa tattgatgtg tcagcaacag cgttttacaa
     3181 ggcacagcca gtaatcgagt ttgtttgtga agttttggat tttaaaagta ttgaagaaca
     3241 acaaaaacct ctgacagatt cccaaagggt aaagtttacc aaagaaatta aaggtctaaa
     3301 ggtggagata acgcactgtg ggcagatgaa gaggaagtac cgtgtctgca atgtgacccg
     3361 gcggcccgcc agtcaccaaa cattcccgct gcagcaggag agcgggcaga cggtggagtg
     3421 cacggtggcc cagtatttca aggacaggca caagttggtt ctgcgctacc cccacctccc
     3481 atgtttacaa gtcggacagg agcagaaaca cacctacctt cccctggagg tctgtaacat
     3541 tgtggcagga caaagatgta ttaaaaaatt aacggacaat cagacctcaa ccatgatcag
     3601 agcgactgct aggtcggcgc ccgatcggca agaagagatt agcaaattga tgcgaagtgc
     3661 aagtttcaac acagatccat acgtccgtga atttggaatc atggtcaaag atgagatgac
     3721 agacgtgact gggcgggtgc tgcagccgcc ctccatcctc tacgggggca ggaataaagc
     3781 tattgcgacc cctgtccagg gcgtctggga catgcggaac aagcagttcc acacgggcat
     3841 cgagatcaag gtgtgggcca ttgcgtgctt cgccccccag cgccagtgca cggaagtcca
     3901 tctgaagtcc ttcacagagc agctcagaaa gatctcgaga gacgccggca tgcccatcca
     3961 gggccagccg tgcttctgca aatacgcgca gggggcggac agcgtggagc ccatgttccg
     4021 gcacctgaag aacacgtatg cgggcctgca gctggtggtg gtcatcctgc ccggcaagac
     4081 gcccgtgtac gccgaggtca agcgcgtggg agacacggtg ctggggatgg ccacgcagtg
     4141 cgtgcagatg aagaacgtgc agaggaccac gccacagacc ctgtccaacc tctgcctgaa
     4201 gatcaacgtc aagctgggag gcgtgaacaa catcctgctg ccccagggca ggccgccggt
     4261 gttccagcag cccgtcatct ttctgggagc agacgtcact cacccccccg ccggggatgg
     4321 gaagaagccc tccattgccg ccgtggtggg cagcatggac gcccacccca atcgctactg
     4381 cgccaccgtg cgcgtgcagc agcaccggca ggagatcata caagacctgg ccgccatggt
     4441 ccgcgagctc ctcatccagt tctacaagtc cacgcgcttc aagcccaccc gcatcatctt
     4501 ctaccgcgac ggtgtctctg aaggccagtt ccagcaggtt ctccaccacg agttgctggc
     4561 catccgtgag gcctgtatca agctagaaaa agactaccag cccgggatca ccttcatcgt
     4621 ggtgcagaag aggcaccaca cccggctctt ctgcactgac aagaacgagc gggttgggaa
     4681 aagtggaaac attccagcag gcacgactgt ggacacgaaa atcacccacc ccaccgagtt
     4741 cgacttctac ctgtgtagtc acgctggcat ccaggggaca agcaggcctt cgcactatca
     4801 cgtcctctgg gacgacaatc gtttctcctc tgatgagctg cagatcctaa cctaccagct
     4861 gtgtcacacc tacgtgcgct gcacacgctc cgtgtccatc ccagcgccag catactacgc
     4921 tcacctggtg gccttccggg ccaggtacca cctggtggat aaggaacatg acagtgctga
     4981 aggaagccat acctctgggc agagtaacgg gcgagaccac caagcactgg ccaaggcggt
     5041 ccaggttcac caagacactc tgcgcaccat gtactttgct tgacggaatt caattgccta
     5101 ggagcgctca cgtgtctaga tagctagcct ccctatagtg agtcgtatta cgtagatcca
     5161 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     5221 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     5281 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     5341 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
     5401 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5461 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag
     5521 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5581 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5641 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5701 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5761 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5821 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5881 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5941 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6001 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6061 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6121 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6181 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6241 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6301 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6361 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6421 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6481 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6541 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6601 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6661 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6721 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6781 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6841 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6901 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6961 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7021 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7081 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7141 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7201 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7261 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7321 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7381 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7441 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7501 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7561 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     7621 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     7681 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     7741 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     7801 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     7861 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     7921 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     7981 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     8041 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     8101 ctcttcgcta ttacgccagt cgaccgccaa ttcaatatgg cgtatatgga ctcatgccaa
     8161 ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa
     8221 ttcaatatgg tggatctgga ccccagccaa ttcaatatgg cggacttggc accatgccaa
     8281 ttcaatatgg cggacctggc actgtgccaa ctggggaggg gtctacttgg cacggtgcca
     8341 agtttgagga ggggtcttgg ccctgtgcca agtccgccat attgaattgg catggtgcca
     8401 ataatggcgg ccatattggc tatatgccag gatcaatata taggcaatat ccaatatggc
     8461 cctatgccaa tatggctatt ggccaggttc aatactatgt attggcccta tgccatatag
     8521 tattccatat atgggttttc ctattgacgt agatagcccc tcccaatggg cggtcccata
     8581 taccatatat ggggcttcct aataccgccc atagccactc ccccattgac gtcaatggtc
     8641 tctatatatg gtctttccta ttgacgtcat atgggcggtc ctattgacgt atatggcgcc
     8701 tcccccattg acgtcaatta cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa
     8761 taggaccacc caccattgac gtcaatggga tggctcattg cccattcata tccgttctca
     8821 cgccccctat tgacgtcaat gacggtaaat ggcccacttg gcagtacatc aatatctatt
     8881 aatagtaact tggcaagtac attactattg gaagtacgcc agggtacatt ggcagtactc
     8941 ccattgacgt caatggcggt aaatggcccg cgatggctgc caagtacatc cccattgacg
     9001 tcaatgggga ggggcaatga cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg
     9061 tgcctaatgg gaggtctata taagcaatgc tcgtttaggg aac
//
