LOCUS       sequence-25998-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Dunderdale HJ, Sharples GJ, Lloyd RG, West SC
  TITLE     Cloning, overexpression, purification, and characterization of the 
            Escherichia coli RuvC Holliday junction resolvase.
  JOURNAL   J Biol Chem. 1994 Feb 18. 269(7):5187-94.
  PUBMED    8106500
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1994 Feb 18. 269(7):5187-94."
COMMENT     Sequence Label: pGS775
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tnctctagaa taattttgtt taactttaag aaggagatat acatatggct agaattcaaa
       61 aaggaggcgc gtgatggcta ttattctcgg cattgatccg ggttcgcgcg tgaccggcta
      121 cggcgtcatc cgccaggtag gtaggcaact gtcctacctg ggtagcggat gcatccgcac
      181 caaagtggat gatttaccgt ctcgtctgaa actcatctat gcgggcgtga cggaaatcat
      241 cacccagttc cagcctgatt atttcgccat tgaacaagtc tttatggcaa agaacgctga
      301 ctcagccctg aaactgggcc aggcgcgcgg cgtggcgatt gtggcggcgg tgaatcagga
      361 gttgccagta tttgaatacg cggcacgtca ggtaaagcaa acggtggtag gtattggcag
      421 tgccgaaaaa agccaggtgc agcatatggt ccgcaccttg ctgaaactgc ccgctaatcc
      481 acaggcggat gccgccgatg cgctggcgat tgctatcacc cactgccacg ttagtcagaa
      541 tgcgatgcag atgagcgaat cgcggctgaa cctggcgaga gggcgactgc gttaagttat
      601 accgcctcgg tcagttccgg ctgaagctta tcgatgataa gctgcntnaa catgaaaaat
      661 taaatcaatc taaagtatat atgagttnac ttggtctgac acagttacca atgcttaatc
      721 aggtggangg nacctaattc tacanccnat tctnnnnttt cgttcatccc ntagttgcct
      781 gacctccccc gtccntggta agatannnan cnaanancnn gaa
//
