LOCUS       sequence-26001-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Lien WH, Gelfand VI, Vasioukhin V
  TITLE     Alpha-E-catenin binds to dynamitin and regulates dynactin-mediated 
            intracellular traffic.
  JOURNAL   J Cell Biol. 2008 Dec 15. 183(6):989-97.
  PUBMED    19075109
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2008 Dec 15. 183(6):989-97."
COMMENT     Sequence Label: pcDNA3.1/nV5-DEST-Apr1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             114..155
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             156..176
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    207..231
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gnngnnnttn gcanagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
       61 attaatacga ctcactatag ggagacccaa gctggctagt taagcttacc atgggtaagc
      121 ctatccctaa ccctctcctc ggtctcgatt ctacggaaaa cctgtatttt cagggcccgg
      181 atccgtcgac gaattctgca gatatcacaa gtttgtacaa aaaagcaggc tccgaattcg
      241 cccttatgga gtcctacgat gtgatcgcca accagcctgt tgtgatcgac aacggatccg
      301 gtgtgattaa agctggcttt gctggtgatc agatccccaa atactgcttt ccaaactatg
      361 tgggcagacc caaacatgtt cgtgtcatgg caggagccct ggaaggtgac atcttcattg
      421 gccccaaagc ggaggagcac cgggggctgc tgtcaatccg ttaccccatg gaacatggca
      481 tcgtcaagga ctggaacgac atggaacgca tctggcaata cgtctactct aaggaccagc
      541 tgcagacttt ctcagaggag catcctgtgc tcctaacgga ggcaccttta aaccctcgga
      601 aaaaccggga gcgagctgca gaagtcttct tcgagacctt caatgtgccg gccctcttca
      661 tctccatgca agccgtgctg agcctttatg ccacgggcag aaccacaggc gtggtgttgg
      721 attctggggg atgggnngnc acccatgcag gtcccgattt atgaaggctt ccccnnnnnc
      781 cacactccan tcangccgnn atncgaacnt cgcnggccgg gagaangnnn ncnacggnt
//
