LOCUS       sequence-260024-        4653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC26A1
FEATURES             Location/Qualifiers
     source          1..4653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2778..2877)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2894..2913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2895..2913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2918..2934)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2918..2934)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3036..3055)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3047..3856
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3114..3133)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4003..4591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4492..4511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggacg agtcccctga gccactgcag cagggaagag gaccagtgcc
      721 tgtgcggaga cagaggccag caccaagagg actgagggag atgctgaagg ccagactgtg
      781 gtgcagctgt tcctgcagcg tgctgtgcgt gcgggccctg gtgcaggacc tgctgccagc
      841 cacccgctgg ctgcggcagt acaggcctcg cgagtatctg gccggcgatg tgatgagcgg
      901 cctggtcatc ggcatcatcc tggtgccaca ggccatcgca tattccctgc tggcaggact
      961 gcagcccatt tactccttgt atacgtcatt ctttgccaac ctgatctact tcctgatggg
     1021 cacctctcgc cacgtgagcg tgggcatctt tagcctgctg tgcctgatgg tgggccaggt
     1081 ggtggacagg gagctgcagc tggcaggctt cgacccatct caggatggac tgcagcctgg
     1141 agccaacagc tccacactga atggcagcgc cgccatgctg gactgtggca gagattgcta
     1201 cgccatcagg gtggccaccg ccctgacact gatgaccggc ctgtatcagg tgctgatggg
     1261 cgtgctgagg ctgggattcg tgtccgccta cctgtctcag cctctgctgg atggctttgc
     1321 catgggcgcc tctgtgacaa tcctgaccag ccagctgaag cacctgctgg gcgtgagaat
     1381 ccctaggcac cagggcccag gcatggtggt gctgacctgg ctgtccctgc tgaggggagc
     1441 aggacaggcc aacgtgtgcg acgtggtgac ctccacagtg tgcctggccg tgctgctggc
     1501 agcaaaggag ctgtctgatc ggtatagaca caggctgcgc gtgcctctgc caacagagct
     1561 gctggtcatc gtggtggcca ccctggtgag ccacttcggc cagctgcaca agagatttgg
     1621 ctctagcgtg gccggcgaca tccccacagg cttcatgcca cctcaggtgc cagagcctag
     1681 gctgatgcag agggtggccc tggatgcagt ggccctggcc ctggtggcag cagccttcag
     1741 catctccctg gccgagatgt ttgcacgcag ccacggatac tccgtgcggg caaaccagga
     1801 gctgctggca gtgggctgct gtaatgtgct gccagccttc ctgcactgtt ttgccacctc
     1861 cgccgccctg gcaaagtctc tggtgaagac cgccacaggc tgcagaacac agctgtcctc
     1921 tgtggtgtcc gccaccgtgg tgctgctggt gctgctggcc ctggcacccc tgtttcacga
     1981 cctgcagagg tctgtgctgg cctgcgtgat cgtggtgagc ctgagaggcg ccctgaggaa
     2041 agtgtgggac ctgccacgcc tgtggcggat gtccccagca gatgcactgg tgtgggcagg
     2101 cacagccgcc acctgcatgc tggtgtctac cgaggcagga ctgctggcag gcgtgatcct
     2161 gtctctgctg agcctggcag gaaggacaca gaggcccaga accgccctgc tggcccggat
     2221 cggcgacaca gccttctatg aggatgccac cgagtttgag ggactggtgc cagagccagg
     2281 cgtgagagtg ttcaggtttg gcggccctct gtactatgcc aacaaggact tctttctgca
     2341 gtccctgtac tctctgacag gactggatgc aggatgtatg gcagcaaggc gcaaggaggg
     2401 cggcagcgag accggagtgg gagagggagg acctgcacag ggagaggatc tgggaccagt
     2461 gtccacaagg gccgccctgg tgcctgcagc agcaggattc cacaccgtgg tcatcgactg
     2521 cgccccactg ctgtttctgg atgcagcagg cgtgagcaca ctgcaggacc tgcggagaga
     2581 ttacggcgcc ctgggaatca gcctgctgct ggcatgctgt tccccacccg tgcgcgacat
     2641 cctgtctagg ggaggcttcc tgggagaggg accaggcgac accgccgagg aggagcagct
     2701 gtttctgagc gtgcacgatg ccgtgcagac agccagggcc cgccaccggg agctggaggc
     2761 caccgatgcc cacctggacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     2821 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2881 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2941 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     3001 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     3061 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     3121 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     3181 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     3241 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     3301 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     3361 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     3421 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     3481 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     3541 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     3601 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     3661 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     3721 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3781 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3841 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     3901 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3961 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4021 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4081 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4141 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4201 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4261 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4321 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4381 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4441 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4501 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4561 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4621 cctggccttt tgctggcctt ttgctcacat gtt
//
