LOCUS       sequence-260030-        6242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human alpha-internexin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Stone EJ, Uchida A, Brown A
  TITLE     Charcot-Marie-Tooth disease Type 2E/1F mutant neurofilament proteins
            assemble into neurofilaments.
  JOURNAL   Cytoskeleton (Hoboken). 2019 Jul;76(7-8):423-439. doi: 
            10.1002/cm.21566. Epub 2019 Nov 6.
  PUBMED    31574566
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Cytoskeleton (Hoboken)."; date: "2019-07"; volume: 
            "76(7-8):423-439. doi"; pages: " 10.1002/cm.21566. Epub 2019 Nov 6"
COMMENT     Sequence Label: phINA
FEATURES             Location/Qualifiers
     source          1..6242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        346..549
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     500..520
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3011..3132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3048..3067)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3102..3121
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3139..3594)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3276..3297)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3488..3507
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3621..3725
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3727..4084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3727..3918
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3750..3770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3935..4070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4119..4913
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4119..4913
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4173..4192)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4783..4802
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5101..5120)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5145..5192
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5521..6109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6010..6029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
       61 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      121 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      181 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      241 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      301 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      361 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      421 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      481 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      541 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
      601 tcaagcttcg aattcggcac gaggcttctg tagctcgcgt tgaagccgca cgtccggccc
      661 cgatcccggc accatgagct tcggctcgga gcactacctg tgctcctcct cctcctaccg
      721 caaggtgttc ggggatggct ctcgcctgtc cgcccgcctc tctggggccg gcggcgcggg
      781 cggcttccgc tcgcagtcgc tgtcccgcag caatgtggcc tcctcggccg cctgctcctc
      841 ggcctcgtcg ctcggcctcg gcctggccta tcgccggccg ccggcgtccg acgggctgga
      901 cctgagccag gcggcggcgc gcaccaacga gtacaagatc atccgcacca acgagaagga
      961 gcagctgcag ggcctcaacg accgcttcgc cgtgttcatc gagaaggtgc atcagctgga
     1021 gacgcagaac cgcgcgttgg aggccgagct ggccgcgctg cgacagcgcc acgctgagcc
     1081 gtcgcgcgtc ggcgagctct tccagcgcga gctgcgcgac ctgcgcgcgc agctggagga
     1141 ggccagctcg gctcgctcgc aggccctgct ggagcgcgac gggctggcgg aggaggtgca
     1201 gcggctgcgg gcgcgctgcg aggaggagag ccgcggacgc gaaggcgccg agcgcgccct
     1261 gaaggcgcag cagcgcgacg tggacggcgc cacgctggcc cgcctggacc tggagaagaa
     1321 ggtggagtcg ctgctggacg agctggcctt cgtacgccag gtgcacgacg aggaggtagc
     1381 cgagctgctg gccacgctgc aggcgtcgtc gcaggccgcg gccgaggtgg acgtgactgt
     1441 ggctaaacca gacctgacct cggctctgag ggagatccgc gcccagtatg agtccctggc
     1501 cgctaagaac ctgcagtccg cggaagaatg gtacaagtcc aagtttgcca acctgaacga
     1561 gcaggcggcg cgcagcaccg aggccatccg ggccagccgc gaggagatcc acgagtatcg
     1621 gcgccagctg caggcgcgca ccatcgagat cgagggcctg cgcggggcca acgagtcctt
     1681 ggagaggcag atcctggagc tggaggagcg gcacagtgcc gaggtagctg gctaccagga
     1741 tagcattggg cagctggaga atgatctgag gaacaccaag agtgagatgg cacgccacct
     1801 tcgggaatac caggacttgc tcaatgtcaa aatggctctt gacattgaga tagcagctta
     1861 caggaaactg ctggaaggcg aggagacacg ttttagcacc agtgggttaa gcatttcggg
     1921 gctgaatcca cttcccaatc caagttacct gctcccacct agaatcctca gtgctacaac
     1981 ctccaaagtc tcatccactg ggctatcact taagaaagag gaggaggagg aggaggcatc
     2041 taaggtagcc tctaagaaaa cctcccagat aggggaaagt tttgaagaaa tattagagga
     2101 gacagtaata tctactaaga aaaccgagaa atcaaatata gaagaaacca ccatttcaag
     2161 ccaaaaaata taattccatt gctttgaaaa agttaatgct taagagggaa tgatatgcat
     2221 ttgacttgtt aaacagccta ttcctgaact ataacacctg ccaccactat aaaatgtctt
     2281 caaggcttca gtctcatatt tagtattgaa tacttacatt ctctaataag aaaaccaccc
     2341 cttagattga agtaaactgc agtcctggag caatcacaga tgagttatct aagaatacag
     2401 ctttctgacc ttcagctcac gcttcacagt gatcgatgat tcaggtgcag aggaagtaca
     2461 aactaaggtg ctaaatctgc gatcatcgtc atttgctgtg aactgaaatt aaaactattc
     2521 atgctaccca gccattaccc agctaaataa tcttactcta gatacctaaa acataagatc
     2581 actgccagag ataaactaat ggtccacacc caattccact ctgatagaat tatttcacaa
     2641 ataataggtg tttgtttaat ggacactttt cacctccttc aattccatat atccttttct
     2701 tctatgtagg aaaaaaatag tctagtgtag tattcttccc ttttaaacac attttggttc
     2761 ttctcaaaag aacttacccc cattcgcgct ctgctcagtg ggtaaaatta gatgcatgtt
     2821 gaccatgtct atgattcgtg taattactta tccctgccca tttcatggta ccgcgggccc
     2881 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
     2941 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3001 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3061 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3121 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     3181 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3241 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3301 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3361 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3421 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3481 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     3541 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     3601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3721 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3781 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3841 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3901 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3961 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4021 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4081 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4141 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4201 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4261 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     4321 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4381 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4441 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4501 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4561 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4621 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     4681 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     4741 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     4801 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     4861 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     4921 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     4981 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5041 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     5101 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     5161 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     5221 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     5281 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     5341 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     5401 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5461 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5521 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5581 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5641 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5701 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5761 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5821 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5881 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5941 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6001 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6061 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6121 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6181 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
     6241 ca
//
