LOCUS       sequence-260049-        7087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains a reporter gene for Crz1. The 1000bp promoter of 
            the gene pYPS1 driving a Venus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Chen SY, Osimiri LC, Chevalier M, Bugaj LJ, Nguyen TH, Greenstein 
            RA, Ng AH, Stewart-Ornstein J, Neves LT, El-Samad H
  TITLE     Optogenetic Control Reveals Differential Promoter Interpretation of 
            Transcription Factor Nuclear Translocation Dynamics.
  JOURNAL   Cell Syst. 2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009.
  PUBMED    32898473
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 
            10.1016/j.cels.2020.08.009."
COMMENT     Sequence Label: pYPS1-Venus
FEATURES             Location/Qualifiers
     source          1..7087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        323..730
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             1914..2627
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2643..2830
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2698..2722)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5432..6247)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5432..6247)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6155..6174
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6239..6258
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6472..7060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6961..6980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
       61 gtggataacc gtgcggccgc caatgaggag aacttctagt atatctacat acctaatatt
      121 attgccttat taaaaatgga atcccaacga ttacatcaaa atccacattc tcttcaaaat
      181 caattgtcct gtacttcctt gttcatgtgt gttcaaaaac gttatattta taggataatt
      241 atactctatt tctcaacaag taattggttg tttggccgag cggtctaagg cgcctgattc
      301 aagaaatatc ttgaccgcag ttaactgtgg gaatactcag gtatcgtaag atgcaagagt
      361 tcgaatctct tagcaaccat tatttttttc ctcaacataa cgagaacaca caggggcgct
      421 atcgcacaga atcaaattcg atgactggaa attttttgtt aatttcagag gtcgcctgac
      481 gcatatacct ttttcaactg aaaaattggg agaaaaagga aaggtgagag cgccggaacc
      541 ggcttttcat atagaataga gaagcgttca tgactaaatg cttgcatcac aatacttgaa
      601 gttgacaata ttatttaagg acctattgtt ttttccaata ggtggttagc aatcgtctta
      661 ctttctaact tttcttacct tttacatttc agcaatatat atatatatat ttcaaggata
      721 taccattcta ccctgaattc gcatctagac tgatgagacg tggtagagcc acaaacagcc
      781 ggtacaagca acgatctcca ggaccatctg aatcatgcgc ggatgacacg aactcacgac
      841 ggcgatcaca gacattaacc cacagtacag acactgcgac aacgtggcaa ttcgtcgcaa
      901 tacaacgcag accagtgtgg ggacctgtgt cttggcaata attccacaag cgggcaacgc
      961 caccgctatc cttggtgatt ccttcttgag aaacgcctac gtggtctacg atttggataa
     1021 ctacgagatt tccctagctc aagccaagta tggcacgggg aaagagaacg tcgaagtcat
     1081 caaatctacc gttcccagtg caataagggc ccccagttac aacaacactt ggtctaacta
     1141 cgcctccgcc acgtccggtg gtaatatttt taccaccgtg cgcactttca atggcaccag
     1201 tactgccacc actacgaggt caaccaccac caagaagaca aactctacca ctactgcaaa
     1261 gtcgactcat aaaagcaaga gggcactcca gagggctgct accaactccg cttccagtat
     1321 acgctctacc ttgggtttac tgctagtccc ctccttactc atcctttccg ttttcttttc
     1381 gtaaaaaaaa acaatagaca ctatatatag acactttttc ctttccttct ttgcgcgatt
     1441 tcaagaggaa aagcatactt aaataagaat attcctaaaa cacacgttct gacgcgtcaa
     1501 ttagatcgtc agaccttgca tgcagccaag acaccctcac atagcactgc ctccttcctc
     1561 ctcttttctg tcaccacctc acctccctcg tccactcaac tgagtggctt ttcgctcctt
     1621 ttatactgcg ccatgagtag ttttcgtttc actgatgtgt ccgaaaaaat tgaggtttca
     1681 taaaaaaatt cgtggactta tttatggaga aacagggaaa tccgactact taagaaaagg
     1741 gtgtcaaaga ggatttactt ttttccttct ttttgcattt gttcctattt ccgcaattgg
     1801 acggttatta agaagaacgc aatttgcttt tctgtatatt aaaatacata gcgtaataaa
     1861 aagataaggt gaacaccaag catatagtat aatattacct accacataga tctatgtcta
     1921 aaggtgaaga attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta
     1981 atggtcacaa attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga
     2041 ccttaaaatt gatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta
     2101 ctttaggtta tggtttgcaa tgttttgcta gatacccaga tcatatgaaa caacatgact
     2161 ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg
     2221 acggtaacta caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa
     2281 tcgaattaaa aggtattgat tttaaagaag gtggtaacat tttaggtcac aaattggaat
     2341 acaactataa ctctcacaat gtttacatca ctgctgacaa acaaaagaat ggtatcaaag
     2401 ctaacttcaa aattagacac aacattgaag atggtggtgt tcaattagct gaccattatc
     2461 aacaaaatac tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcct
     2521 atcaatctgc cttatccaaa gatccaaacg aaaagagaga tcacatggtc ttgttagaat
     2581 ttgttactgc tgctggtatt acccatggta tggatgaatt gtacaaagga tcctaactcg
     2641 aggcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     2701 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     2761 ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca
     2821 cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttcgct ggaaatctgc
     2881 tcgtcagtgg tgctcacact gacgaatcat gtacagatca taccgatgac tgcctggcga
     2941 ctcacaacta agcaagacag ccggaaccag cgccggcgaa caccactgca tatatggcat
     3001 atcacaacag tccacgtctc aagcagttac agagatgtta cgaaccacta gtgcactgca
     3061 gtacattacc aatagatagc ggtttaatat aaggtttcaa caagaaatat tttgtgaaaa
     3121 tcaaaagtta atagtttcac tgtcgaaaat atgaaagttt aagttttcag ttcatcccat
     3181 tgtagttatc aaatttctgt gtttcccgta tatgtgtgct ctgtattcat cgctatcttg
     3241 aaactggtta tgatcgttgc gcatgtgacc tgtatcgctc aaaagagccc ggcaccggta
     3301 ccgggcaaaa gcccctagca agctaaaatg attcaacttt cgtagtaatt tgttcaaccg
     3361 gtcacagtaa agtcaatgac taacttttga atcgaggatg tatttccaat tgaaagcaat
     3421 gtatatataa accattgtgt tgaagcttgt cttacagttt tcttgtatcc tcccattgtt
     3481 catatagtac ttaaagtacc ataatcctta aagaacaaac ttgaaagaga atacactata
     3541 cacaactaat aaataaagaa taatcatggc tgtgaccaag acaattgtag ttctaccagg
     3601 tgaccatgtt ggtcaagaaa tcactgaaga ggccattaaa gttttaaatg ctattcagga
     3661 atgtcgtcca gacaaggtca atttcaagtt tgatcatcat ttgatcggtg gtgctgcaat
     3721 tgatgccact ggtgttccat taccagacga agctttggag gcctctaaga aagctgatgc
     3781 cgtgcttctg ggtgctgttg gtggtccaaa atggggtact ggcgctgtca gaccagaaca
     3841 aggtcttttg aaaatccgta aggagttgca attgtatgct aatctaagac catgtaattt
     3901 tgcatctgat tccttactag atctatcgcc attgaagcct gaaattgcaa gaggtacaga
     3961 tttcgtcgtt gttagagaac tagtgggtgg tatttatttc ggggagagaa aagaagatga
     4021 aggtgatggt gtcgcctggg atagcgaaaa gtattctgtg cccgaagttc aaagaatcac
     4081 tagaatggct gctttcatgg ctctgcaaca caacccacct ttacctattt ggactctaga
     4141 taaagccaac gttctagcat catcaagatt atggagaaaa acagtggaaa agacgattaa
     4201 gaatgaattc ccacaattga ctgttcaaca tcaattgatt gattccgctg ctatgatctt
     4261 ggttaagaac ccaactcatc taaatggtat tattatcaca aacaatatgt ttggtgatat
     4321 tatctccgat gaagcctccg tgatcccagg ttcgttagga ttacttccat ctgcctcctt
     4381 ggcatctctt ccagataaga atacggcatt tggtttatat gagccatgtc atggctctgc
     4441 tccagactta ccaaagggaa aggttaaccc cgtagcaaca atcctatctg ctgctatgat
     4501 gttgaagttg tctctggacc tgttcgaaga aggtgaaatt atagaacaag cagtcaagaa
     4561 agtgttggat tctggtatca gaacagcgga tctaaagggt accaactcta ctactgaggt
     4621 cggtgatgca gtagcgaaag ctgtcaggga actattagct tagacttagt agatgtacag
     4681 ttgcatacat aagtctgaac agaaagaagt aaatatcata tctagttcgg tttagaaatc
     4741 agtgactata ttggttttga ccagatcgca atccttgcaa gcatttagta atgactatca
     4801 aaccggtgaa tcatctgata aggctatatt agcttgtgca ttcgcatgta tcgggaaacg
     4861 aacgagtcca ccgaagtcgg tgatgctgtc gccgaagaag ttaagaaaat ccttgcttaa
     4921 aaagattctc tttttttatg atatttgtac ataaacttta taaatgaaat tcataataga
     4981 aacgacacga aattacaaaa tggaatatgt tcatagggta gacgaaacta tatacgcaat
     5041 ctacatacat ttatcaagaa ggagaaaaag gaggatgtaa aggaatacag gtaagcaaat
     5101 tgatactaat ggctcaacgt gataaggaaa aagaattgca ctttaacatt aatattgaca
     5161 aggaggaggg caccacacaa aaagttaggt gtaacagaaa atcatgaaac tatgattcct
     5221 aatttatata ttggaggatt ttctctaaaa aaaaaaaaat acaacaaata aaaaacactc
     5281 aatgacctga ccatttgatg gagtttaagt caatacctcc ttgaaccatt tcccataatg
     5341 gtgaaagttc cctcaagaat tttactctgt cagaaacggc cttaacgacg taccgagcgg
     5401 ccgcgtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     5461 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     5521 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     5581 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     5641 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
     5701 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     5761 catcaaccaa accgttattc attcgtgatt gcgcctgagc gaggcgaaat acgcgatcgc
     5821 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     5881 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     5941 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     6001 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     6061 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     6121 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     6181 aatcagcatc catgttggaa tttaatcgcg gcctggagca agacgtttcc cgttgaatat
     6241 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     6301 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt
     6361 gaataaatcg aacttttgct gagttgaagg atcagtcatg accaaaatcc cttaacgtga
     6421 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6481 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6541 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6601 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6661 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6721 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6781 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6841 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6901 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6961 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7021 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7081 tttacgg
//
