LOCUS       sequence-260061-       11996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Thy1.1 and FOXO3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Krummey SM, Hartigan CR, Liu D, Ford ML
  TITLE     CD28-Dependent CTLA-4 Expression Fine-Tunes the Activation of Human 
            Th17 Cells.
  JOURNAL   iScience. 2020 Apr 24;23(4):100912. doi: 10.1016/j.isci.2020.100912.
            Epub 2020 Feb 14.
  PUBMED    32203908
REFERENCE   2  (bases 1 to 11996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-04-24"; volume: "23(4):100912. doi"; pages: " 
            10.1016/j.isci.2020.100912. Epub 2020 Feb 14"
COMMENT     Sequence Label: pCMJJ4-FOXO3-IRES-Thy1.1
FEATURES             Location/Qualifiers
     source          1..11996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        279..582
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        583..786
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     737..757
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     783..807
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      892..901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2967..3533
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2983..3535
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3127..3144)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3354..3373
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3583..4071
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    4099..4687
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4152..4172)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4570..4581)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4690..4706)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4691..4707)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4797..5030
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5055..5073)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5056..5073)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5363..5382)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5596..5615)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5715..5737
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5775..5793)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5861..5965
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5966..6034,6035..6826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6184..6203)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6997..7585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7486..7505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7739..7756
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7826..7846)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7831..8160
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8011..8146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9294..9359
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9489..9509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9934..10068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9971..9990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10025..10044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10236..10869
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10916..11041
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11534..11767
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11952..11996
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
       61 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
      121 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
      181 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
      241 aacggatctc gacggtatcg ataagcttgg gagttccgcg ttacataact tacggtaaat
      301 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      361 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      421 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      481 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      541 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      601 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      661 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      721 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      781 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      841 catagaagac accgactcta gctagaggat catttaaatt taattaagtt tgccaccatg
      901 gcagaggcac cggcttcccc ggccccgctc tctccgctcg aagtggagct ggacccggag
      961 ttcgagcccc agagccgtcc gcgatcctgt acgtggcccc tgcaaaggcc ggagctccaa
     1021 gcgagccctg ccaagccctc gggggagacg gccgctgact ccatgatccc cgaggaggag
     1081 gacgatgaag acgacgagga cggcggggga cgggccggct cggccatggc gatcggcggc
     1141 ggcggcggga gcggcacgct gggctccggg ctgctccttg aggactcggc ccgggtgctg
     1201 gcacccggag ggcaagaccc cgggtctggg ccagccaccg cggcgggcgg gctgagcggg
     1261 ggtacacagg cgctgctgca gcctcagcaa ccgctgccac cgccgcagcc gggggcggct
     1321 gggggctccg ggcagccgag gaaatgttcg tcgcggcgga acgcctgggg aaacctgtcc
     1381 tacgcggacc tgatcacccg cgccatcgag agctccccgg acaaacggct cactctgtcc
     1441 cagatctacg agtggatggt gcgttgcgtg ccctacttca aggataaggg cgacagcaac
     1501 agctctgccg gctggaagaa ctccatccgg cacaacctgt cactgcatag tcgattcatg
     1561 cgggtccaga atgagggaac tggcaagagc tcttggtgga tcatcaaccc tgatgggggg
     1621 aagagcggaa aagccccccg gcggcgggct gtctccatgg acaatagcaa caagtatacc
     1681 aagagccgtg gccgcgcagc caagaagaag gcagccctgc agacagcccc cgaatcagct
     1741 gacgacagtc cctcccagct ctccaagtgg cctggcagcc ccacgtcacg cagcagtgat
     1801 gagctggatg cgtggacgga cttccgttca cgcaccaatt ctaacgccag cacagtcagt
     1861 ggccgcctgt cgcccatcat ggcaagcaca gagttggatg aagtccagga cgatgatgcg
     1921 cctctctcgc ccatgctcta cagcagctca gccagcctgt caccttcagt aagcaagccg
     1981 tgcacggtgg aactgccacg gctgactgat atggcaggca ccatgaatct gaatgatggg
     2041 ctgactgaaa acctcatgga cgacctgctg gataacatca cgctcccgcc atcccagcca
     2101 tcgcccactg ggggactcat gcagcggagc tctagcttcc cgtataccac caagggctcg
     2161 ggcctgggct ccccaaccag ctcctttaac agcacggtgt tcggaccttc atctctgaac
     2221 tccctacgcc agtctcccat gcagaccatc caagagaaca agccagctac cttctcttcc
     2281 atgtcacact atggtaacca gacactccag gacctgctca cttcggactc acttagccac
     2341 agcgatgtca tgatgacaca gtcggacccc ttgatgtctc aggccagcac cgctgtgtct
     2401 gcccagaatt cccgccggaa cgtgatgctt cgcaatgatc cgatgatgtc ctttgctgcc
     2461 cagcctaacc agggaagttt ggtcaatcag aacttgctcc accaccagca ccaaacccag
     2521 ggcgctcttg gtggcagccg tgccttgtcg aattctgtca gcaacatggg cttgagtgag
     2581 tccagcagcc ttgggtcagc caaacaccag cagcagtctc ctgtcagcca gtctatgcaa
     2641 accctctcgg actctctctc aggctcctcc ttgtactcaa ctagtgcaaa cctgcccgtc
     2701 atgggccatg agaagttccc cagcgacttg gacctggaca tgttcaatgg gagcttggaa
     2761 tgtgacatgg agtccattat ccgtagtgaa ctcatggatg ctgatgggtt ggattttaac
     2821 tttgattccc tcatctccac acagaatgtt gttggtttga acgtggggaa cttcactggt
     2881 gctaagcagg cctcatctca gagctgggtg ccaggctaag gatccaaacg atcccctcga
     2941 cggtatcgat aagcttgata tcgaattccg cccccccccc taacgttact ggccgaagcc
     3001 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     3061 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     3121 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     3181 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     3241 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     3301 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     3361 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     3421 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     3481 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     3541 acaaccatgg gtcttttctg cagtcaccgt cctcgaggca ccatgaaccc agccatcagc
     3601 gtcgctctcc tgctctcagt cttgcaggtg tcccgagggc agaaggtgac cagcctgaca
     3661 gcctgcctgg tgaaccaaaa ccttcgcctg gactgccgcc atgagaataa caccaaggat
     3721 aactccatcc agcatgagtt cagcctgacc cgagagaaga ggaagcacgt gctctcaggc
     3781 acccttggga tacccgagca cacgtaccgc tcccgcgtca ccctctccaa ccagccctat
     3841 atcaaggtcc ttaccctagc caacttcacc accaaggatg agggcgacta cttttgtgag
     3901 cttcgcgtct cgggcgcgaa tcccatgagc tccaataaaa gtatcagtgt gtatagagac
     3961 aagctggtca agtgtggcgg cataagcctg ctggttcaga acacatcctg gatgctgctg
     4021 ctgctgcttt ccctctccct cctccaagcc ctggacttca tttctctgtg acgcgaattc
     4081 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4141 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4201 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4261 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4321 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4381 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4441 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4501 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4561 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4621 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4681 tccccgcatc gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc
     4741 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4801 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     4861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     5041 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     5101 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     5161 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     5221 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     5281 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     5341 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     5401 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     5461 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     5521 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     5581 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     5641 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5701 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     5761 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5821 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5881 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5941 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6001 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6061 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6121 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6181 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6241 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6301 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6361 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6421 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6481 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6541 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6601 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6661 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6721 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6781 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6841 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6901 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6961 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7021 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7081 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7141 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7201 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7261 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7321 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7381 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7441 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7501 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7561 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7621 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7681 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7741 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7801 ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7861 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7921 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7981 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8041 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8101 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8161 aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc
     8221 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
     8281 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
     8341 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
     8401 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
     8461 cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat
     8521 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg
     8581 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt
     8641 ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca
     8701 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg
     8761 attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct
     8821 tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt
     8881 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt
     8941 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc
     9001 acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat
     9061 taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc
     9121 acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg
     9181 ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact
     9241 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     9301 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     9361 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     9421 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     9481 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     9541 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     9601 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
     9661 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
     9721 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
     9781 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
     9841 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     9901 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     9961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    10081 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    10141 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    10201 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
    10261 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
    10321 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
    10381 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
    10441 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
    10501 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
    10561 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
    10621 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
    10681 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    10741 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10801 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10861 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
    10921 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
    10981 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
    11041 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
    11101 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
    11161 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
    11221 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
    11281 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    11341 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
    11401 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    11461 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    11521 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    11581 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    11641 agcagaacaa tttgctgaag gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    11701 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    11761 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    11821 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    11881 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    11941 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttg
//
