LOCUS       sequence-260062-        9962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Thy1.1 only.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Krummey SM, Hartigan CR, Liu D, Ford ML
  TITLE     CD28-Dependent CTLA-4 Expression Fine-Tunes the Activation of Human 
            Th17 Cells.
  JOURNAL   iScience. 2020 Apr 24;23(4):100912. doi: 10.1016/j.isci.2020.100912.
            Epub 2020 Feb 14.
  PUBMED    32203908
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-04-24"; volume: "23(4):100912. doi"; pages: " 
            10.1016/j.isci.2020.100912. Epub 2020 Feb 14"
COMMENT     Sequence Label: pCMJJ4-Thy1.1
FEATURES             Location/Qualifiers
     source          1..9962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        279..582
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        583..786
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     737..757
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     783..807
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    933..1499
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    949..1501
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1093..1110)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1320..1339
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1549..2037
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    2065..2653
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2118..2138)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2536..2547)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2656..2672)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2657..2673)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2763..2996
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3021..3039)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3022..3039)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3329..3348)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3562..3581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3681..3703
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3741..3759)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3827..3931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3932..4000,4001..4792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4150..4169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4963..5551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5452..5471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5705..5722
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5792..5812)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5797..6126
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5977..6112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7260..7325
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7455..7475
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7900..8034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7937..7956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7991..8010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8202..8835
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8882..9007
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9500..9733
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9918..9962
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
       61 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
      121 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
      181 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
      241 aacggatctc gacggtatcg ataagcttgg gagttccgcg ttacataact tacggtaaat
      301 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      361 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      421 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      481 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      541 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      601 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      661 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      721 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      781 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      841 catagaagac accgactcta gctagaggat catttaaatt taattaagtt taaacgatcc
      901 cctcgacggt atcgataagc ttgatatcga attccgcccc cccccctaac gttactggcc
      961 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     1021 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     1081 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     1141 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     1201 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     1261 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     1321 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     1381 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     1441 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     1501 atggccacaa ccatgggtct tttctgcagt caccgtcctc gaggcaccat gaacccagcc
     1561 atcagcgtcg ctctcctgct ctcagtcttg caggtgtccc gagggcagaa ggtgaccagc
     1621 ctgacagcct gcctggtgaa ccaaaacctt cgcctggact gccgccatga gaataacacc
     1681 aaggataact ccatccagca tgagttcagc ctgacccgag agaagaggaa gcacgtgctc
     1741 tcaggcaccc ttgggatacc cgagcacacg taccgctccc gcgtcaccct ctccaaccag
     1801 ccctatatca aggtccttac cctagccaac ttcaccacca aggatgaggg cgactacttt
     1861 tgtgagcttc gcgtctcggg cgcgaatccc atgagctcca ataaaagtat cagtgtgtat
     1921 agagacaagc tggtcaagtg tggcggcata agcctgctgg ttcagaacac atcctggatg
     1981 ctgctgctgc tgctttccct ctccctcctc caagccctgg acttcatttc tctgtgacgc
     2041 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2101 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2161 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2221 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2281 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2341 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2401 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2461 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     2521 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     2581 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     2641 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     2701 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     2761 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     2821 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     2881 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     2941 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     3001 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     3061 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     3121 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     3181 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     3241 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     3301 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     3361 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     3421 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     3481 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     3541 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     3601 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3661 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3721 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3781 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3841 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3901 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3961 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4021 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4081 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4141 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4201 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4261 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4321 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4381 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4441 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4501 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4561 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4621 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4681 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4741 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4801 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4861 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4921 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4981 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5041 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5101 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5161 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5221 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5281 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5341 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5401 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5461 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5521 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5581 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5641 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5701 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5761 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     5821 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5881 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5941 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6001 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6061 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     6121 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     6181 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     6241 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     6301 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     6361 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     6421 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     6481 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     6541 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     6601 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     6661 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     6721 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     6781 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     6841 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     6901 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     6961 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     7021 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     7081 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     7141 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     7201 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     7261 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     7321 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     7381 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     7441 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     7501 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     7561 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     7621 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     7681 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     7741 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     7801 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     7861 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     7921 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7981 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     8041 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     8101 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     8161 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     8221 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     8281 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     8341 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     8401 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     8461 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     8521 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     8581 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     8641 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8701 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8761 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8821 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8881 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8941 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     9001 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     9061 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     9121 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     9181 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     9241 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     9301 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     9361 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     9421 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     9481 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     9541 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     9601 tgcagcagca gaacaatttg ctgaaggcta ttgaggcgca acagcatctg ttgcaactca
     9661 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9721 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9781 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     9841 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     9901 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     9961 tg
//
