LOCUS       sequence-260066-       11942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Thy1.1 and FOXO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11942)
  AUTHORS   Krummey SM, Hartigan CR, Liu D, Ford ML
  TITLE     CD28-Dependent CTLA-4 Expression Fine-Tunes the Activation of Human 
            Th17 Cells.
  JOURNAL   iScience. 2020 Apr 24;23(4):100912. doi: 10.1016/j.isci.2020.100912.
            Epub 2020 Feb 14.
  PUBMED    32203908
REFERENCE   2  (bases 1 to 11942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-04-24"; volume: "23(4):100912. doi"; pages: " 
            10.1016/j.isci.2020.100912. Epub 2020 Feb 14"
COMMENT     Sequence Label: pCMJJ4-FOXO1-IRES-Thy1.1
FEATURES             Location/Qualifiers
     source          1..11942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        279..582
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        583..786
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     737..757
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     783..807
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      892..901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2913..3479
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2929..3481
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3073..3090)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3300..3319
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3529..4017
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    4045..4633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4098..4118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4516..4527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4636..4652)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4637..4653)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4743..4976
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5001..5019)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5002..5019)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5309..5328)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5542..5561)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5661..5683
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5721..5739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5807..5911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5912..5980,5981..6772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6130..6149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6943..7531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7432..7451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7685..7702
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7772..7792)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7777..8106
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7957..8092
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9240..9305
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9435..9455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9880..10014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9917..9936)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9971..9990
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10182..10815
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10862..10987
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11480..11713
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11898..11942
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
       61 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
      121 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
      181 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
      241 aacggatctc gacggtatcg ataagcttgg gagttccgcg ttacataact tacggtaaat
      301 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      361 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      421 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      481 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      541 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      601 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      661 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      721 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      781 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      841 catagaagac accgactcta gctagaggat catttaaatt taattaagtt tgccaccatg
      901 gccgaggcgc ctcaggtggt ggagatcgac ccggacttcg agccgctgcc ccggccgcgc
      961 tcgtgcacct ggccgctgcc caggccggag tttagccagt ccaactcggc cacctccagc
     1021 ccggcgccgt cgggcagcgc ggctgccaac cccgacgccg cggcgggcct gccctcggcc
     1081 tcggctgccg ctgtcagcgc cgacttcatg agcaacctga gcttgctgga ggagagcgag
     1141 gacttcccgc aggcgcccgg ctccgtggcg gcggcggtgg cggcggcggc cgccgcggcc
     1201 gccaccgggg ggctgtgcgg ggacttccag ggcccggagg cgggctgcct gcacccagcg
     1261 ccaccgcagc ccccgccgcc cgggccgctg tcgcagcacc cgccggtgcc ccccgccgcc
     1321 gctgggccgc tcgcggggca gccgcgcaag agcagctcgt cccgccgcaa cgcgtggggc
     1381 aacctgtcct acgccgacct catcaccaag gccatcgaga gctcggcgga gaagcggctc
     1441 acgctgtcgc agatctacga gtggatggtc aagagcgtgc cctacttcaa ggataagggt
     1501 gacagcaaca gctcggcggg ctggaagaat tcaattcgtc ataatctgtc cctacacagc
     1561 aagttcattc gtgtgcagaa tgaaggaact ggaaaaagtt cttggtggat gctcaatcca
     1621 gagggtggca agagcgggaa atctcctagg agaagagctg catccatgga caacaacagt
     1681 aaatttgcta agagccgaag ccgagctgcc aagaagaaag catctctcca gtctggccag
     1741 gagggtgctg gggacagccc tggatcacag ttttccaaat ggcctgcaag ccctggctct
     1801 cacagcaatg atgactttga taactggagt acatttcgcc ctcgaactag ctcaaatgct
     1861 agtactatta gtgggagact ctcacccatt atgaccgaac aggatgatct tggagaaggg
     1921 gatgtgcatt ctatggtgta cccgccatct gccgcaaaga tggcctctac tttacccagt
     1981 ctgtctgaga taagcaatcc cgaaaacatg gaaaatcttt tggataatct caaccttctc
     2041 tcatcaccaa catcattaac tgtttcgacc cagtcctcac ctggcaccat gatgcagcag
     2101 acgccgtgct actcgtttgc gccaccaaac accagtttga attcacccag cccaaactac
     2161 caaaaatata catatggcca atccagcatg agccctttgc cccagatgcc tatacaaaca
     2221 cttcaggaca ataagtcgag ttatggaggt atgagtcagt ataactgtgc gcctggactc
     2281 ttgaaggagt tgctgacttc tgactctcct ccccataatg acattatgac accagttgat
     2341 cctggggtag cccagcccaa cagccgggtt ctgggccaga acgtcatgat gggccctaat
     2401 tcggtcatgt caacctatgg cagccaggta tctcataaca aaatgatgaa tcccagctcc
     2461 catacccacc ctggacatgc tcagcagaca tctgcagtta acgggcgtcc cctgccccac
     2521 acggtaagca ccatgcccca cacctcgggt atgaaccgcc tgacccaagt gaagacacct
     2581 gtacaagtgc ctctgcccca ccccatgcag atgagtgccc tggggggcta ctcctccgtg
     2641 agcagctgca atggctatgg cagaatgggc cttctccacc aggagaagct cccaagtgac
     2701 ttggatggca tgttcattga gcgcttagac tgtgacatgg aatccatcat tcggaatgac
     2761 ctcatggatg gagatacatt ggattttaac tttgacaatg tgttgcccaa ccaaagcttc
     2821 ccacacagtg tcaagacaac gacacatagc tgggtgtcag gctaaggatc caaacgatcc
     2881 cctcgacggt atcgataagc ttgatatcga attccgcccc cccccctaac gttactggcc
     2941 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     3001 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     3061 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     3121 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     3181 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     3241 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     3301 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     3361 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     3421 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     3481 atggccacaa ccatgggtct tttctgcagt caccgtcctc gaggcaccat gaacccagcc
     3541 atcagcgtcg ctctcctgct ctcagtcttg caggtgtccc gagggcagaa ggtgaccagc
     3601 ctgacagcct gcctggtgaa ccaaaacctt cgcctggact gccgccatga gaataacacc
     3661 aaggataact ccatccagca tgagttcagc ctgacccgag agaagaggaa gcacgtgctc
     3721 tcaggcaccc ttgggatacc cgagcacacg taccgctccc gcgtcaccct ctccaaccag
     3781 ccctatatca aggtccttac cctagccaac ttcaccacca aggatgaggg cgactacttt
     3841 tgtgagcttc gcgtctcggg cgcgaatccc atgagctcca ataaaagtat cagtgtgtat
     3901 agagacaagc tggtcaagtg tggcggcata agcctgctgg ttcagaacac atcctggatg
     3961 ctgctgctgc tgctttccct ctccctcctc caagccctgg acttcatttc tctgtgacgc
     4021 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4081 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4141 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4201 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4261 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4321 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4381 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4441 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4501 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4561 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4621 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     4681 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4741 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4801 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4861 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4921 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     4981 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     5041 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     5101 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     5161 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     5221 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     5281 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     5341 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     5401 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     5461 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     5521 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     5581 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5641 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5701 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5761 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5821 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5881 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5941 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6001 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6061 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     6121 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     6181 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6241 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6301 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6361 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6421 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6481 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6541 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6601 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6661 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6721 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6781 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6841 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6901 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6961 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7021 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7081 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7141 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7201 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7261 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7321 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7381 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7441 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7501 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7561 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7621 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7681 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7741 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     7801 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7861 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7921 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7981 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8041 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8101 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     8161 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     8221 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     8281 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     8341 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     8401 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     8461 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     8521 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     8581 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     8641 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     8701 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     8761 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     8821 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     8881 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     8941 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     9001 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     9061 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     9121 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     9181 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     9241 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     9301 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     9361 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     9421 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     9481 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     9541 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     9601 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     9661 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     9721 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     9781 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     9841 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     9901 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9961 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    10021 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    10081 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    10141 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
    10201 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
    10261 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
    10321 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
    10381 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
    10441 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
    10501 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
    10561 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
    10621 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    10681 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    10741 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    10801 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
    10861 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
    10921 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
    10981 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
    11041 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
    11101 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
    11161 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
    11221 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
    11281 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
    11341 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
    11401 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
    11461 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
    11521 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
    11581 tgcagcagca gaacaatttg ctgaaggcta ttgaggcgca acagcatctg ttgcaactca
    11641 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
    11701 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
    11761 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    11821 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    11881 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    11941 tg
//
