LOCUS       sequence-260068-        5287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway vector for expression of proteins in Xenopus oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Chen LQ, Hou BH, Lalonde S, Takanaga H, Hartung ML, Qu XQ, Guo WJ, 
            Kim JG, Underwood W, Chaudhuri B, Chermak D, Antony G, White FF, 
            Somerville SC, Mudgett MB, Frommer WB
  TITLE     Sugar transporters for intercellular exchange and nutrition of 
            pathogens.
  JOURNAL   Nature. 2010 Nov 25;468(7323):527-32. doi: 10.1038/nature09606.
  PUBMED    21107422
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "527-32"
COMMENT     Sequence Label: pOO2-GW
FEATURES             Location/Qualifiers
     source          1..5287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..61
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     43..60
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     5'UTR           73..115
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     protein_bind    159..283
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(712..1017)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(814..833)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1337..2020)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     1935..1954
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2021..2123)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2301..2425)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     3'UTR           2492..2617
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     rep_origin      2840..3295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2927..2946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3137..3158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3324..3428
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3429..3497,3498..4289)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3647..3666)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4460..5048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4949..4968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5202..5219
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
       61 aatacaagct tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcaggg
      121 atccgggccc cgggttaact agtgaattcg cggccatcac aagtttgtac aaaaaagctg
      181 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
      241 agactacata atactgtaaa acacaacata tccagtcact atgaatcaac tacttagatg
      301 gtattagtga cctgtagtcg accgacagcc ttccaaatgt tcttcgggtg atgctgccaa
      361 cttagtcgac cgacagcctt ccaaatgttc ttctcaaacg gaatcgtcgt atccagccta
      421 ctcgctattg tcctcaatgc cgtattaaat cataaaaaga aataagaaaa agaggtgcga
      481 gcctcttttt tgtgtgacaa aataaaaaca tctacctatt catatacgct agtgtcatag
      541 tcctgaaaat catctgcatc aagaacaatt tcacaactct tatacttttc tcttacaagt
      601 cgttcggctt catctggatt ttcagcctct atacttacta aacgtgataa agtttctgta
      661 atttctactg tatcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc
      721 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
      781 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct
      841 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg
      901 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac
      961 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc
     1021 accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag
     1081 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct
     1141 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata
     1201 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata
     1261 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca
     1321 tactgttatc tggcttttag taagccggat ccacgcggcg tttacgcccc ccctgccact
     1381 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1441 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1501 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1561 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     1621 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1681 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1741 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1801 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1861 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1921 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1981 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2041 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     2101 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
     2161 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
     2221 tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt agtcgactac aggtcactaa
     2281 taccatctaa gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag
     2341 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     2401 gttcagcttt cttgtacaaa gtggtgtagt taattcatga tggccgctgc aggtcgacct
     2461 cgagatctgc agctggtacc ggttaccact aaaccagcct caagaacacc cgaatggagt
     2521 ctctaagcta cataatacca acttacactt tacaaaatgt tgtcccccaa aatgtagcca
     2581 ttcgtatctg ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa
     2641 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2701 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aacgcgtcac
     2761 gtgccctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2821 ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2881 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2941 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3001 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3061 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3121 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3181 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3241 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccag
     3301 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3361 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3421 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3481 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3541 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3601 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3661 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3721 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3781 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3841 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3901 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3961 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4021 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4081 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4141 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4201 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4261 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4321 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4381 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4441 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4501 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4561 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     4621 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4681 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4741 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4801 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4861 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4921 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4981 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5041 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5101 ctcacatgtt ctttcctgcg ttattccctg attctgtgga taaccgtatt accgcctttg
     5161 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5221 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5281 gcagccg
//
