LOCUS       sequence-260085-        8663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for catalytically inactive Cpf1 from 
            Lachnospiraceae bacterium (dLbCpf1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman
            JS, Joung JK
  TITLE     Inducible and multiplex gene regulation using CRISPR-Cpf1-based 
            transcription factors.
  JOURNAL   Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
  PUBMED    29083402
REFERENCE   2  (bases 1 to 8663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483."
COMMENT     Sequence Label: MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA
FEATURES             Location/Qualifiers
     source          1..8663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          661..1677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1685..1704
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1776..5459
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5460..5507
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5514..5594
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5684..5703)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5749..5804
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5803..5822)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6165..6181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6165..6181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6178..6200)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6189..6205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6213..6219,6220..6237,6238..6243))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6258..6279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6338..6533
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6384..6519
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6539..6673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6576..6595)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6630..6649
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6741..6758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6912..7500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6992..7011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7671..8462,8463..8531))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8294..8313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8532..8636)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg ccrggggggg gggggggggg gggggggggg
      541 gggggggsgr ggsggrgrgg kgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccggggr gggctcgggg grggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ctgcagtcga
     1741 cggtaccgcg ggcccgggat ccaccggtcg ccaccatgag caagctggag aagtttacaa
     1801 actgctactc cctgtctaag accctgaggt tcaaggccat ccctgtgggc aagacccagg
     1861 agaacatcga caataagcgg ctgctggtgg aggacgagaa gagagccgag gattataagg
     1921 gcgtgaagaa gctgctggat cgctactatc tgtcttttat caacgacgtg ctgcacagca
     1981 tcaagctgaa gaatctgaac aattacatca gcctgttccg gaagaaaacc agaaccgaga
     2041 aggagaataa ggagctggag aacctggaga tcaatctgcg gaaggagatc gccaaggcct
     2101 tcaagggcaa cgagggctac aagtccctgt ttaagaagga tatcatcgag acaatcctgc
     2161 cagagttcct ggacgataag gacgagatcg ccctggtgaa cagcttcaat ggctttacca
     2221 cagccttcac cggcttcttt gataacagag agaatatgtt ttccgaggag gccaagagca
     2281 catccatcgc cttcaggtgt atcaacgaga atctgacccg ctacatctct aatatggaca
     2341 tcttcgagaa ggtggacgcc atctttgata agcacgaggt gcaggagatc aaggagaaga
     2401 tcctgaacag cgactatgat gtggaggatt tctttgaggg cgagttcttt aactttgtgc
     2461 tgacacagga gggcatcgac gtgtataacg ccatcatcgg cggcttcgtg accgagagcg
     2521 gcgagaagat caagggcctg aacgagtaca tcaacctgta taatcagaaa accaagcaga
     2581 agctgcctaa gtttaagcca ctgtataagc aggtgctgag cgatcgggag tctctgagct
     2641 tctacggcga gggctataca tccgatgagg aggtgctgga ggtgtttaga aacaccctga
     2701 acaagaacag cgagatcttc agctccatca agaagctgga gaagctgttc aagaattttg
     2761 acgagtactc tagcgccggc atctttgtga agaacggccc cgccatcagc acaatctcca
     2821 aggatatctt cggcgagtgg aacgtgatcc gggacaagtg gaatgccgag tatgacgata
     2881 tccacctgaa gaagaaggcc gtggtgaccg agaagtacga ggacgatcgg agaaagtcct
     2941 tcaagaagat cggctccttt tctctggagc agctgcagga gtacgccgac gccgatctgt
     3001 ctgtggtgga gaagctgaag gagatcatca tccagaaggt ggatgagatc tacaaggtgt
     3061 atggctcctc tgagaagctg ttcgacgccg attttgtgct ggagaagagc ctgaagaaga
     3121 acgacgccgt ggtggccatc atgaaggacc tgctggattc tgtgaagagc ttcgagaatt
     3181 acatcaaggc cttctttggc gagggcaagg agacaaacag ggacgagtcc ttctatggcg
     3241 attttgtgct ggcctacgac atcctgctga aggtggacca catctacgat gccatccgca
     3301 attatgtgac ccagaagccc tactctaagg ataagttcaa gctgtatttt cagaaccctc
     3361 agttcatggg cggctgggac aaggataagg agacagacta tcgggccacc atcctgagat
     3421 acggctccaa gtactatctg gccatcatgg ataagaagta cgccaagtgc ctgcagaaga
     3481 tcgacaagga cgatgtgaac ggcaattacg agaagatcaa ctataagctg ctgcccggcc
     3541 ctaataagat gctgccaaag gtgttctttt ctaagaagtg gatggcctac tataacccca
     3601 gcgaggacat ccagaagatc tacaagaatg gcacattcaa gaagggcgat atgtttaacc
     3661 tgaatgactg tcacaagctg atcgacttct ttaaggatag catctcccgg tatccaaagt
     3721 ggtccaatgc ctacgatttc aacttttctg agacagagaa gtataaggac atcgccggct
     3781 tttacagaga ggtggaggag cagggctata aggtgagctt cgagtctgcc agcaagaagg
     3841 aggtggataa gctggtggag gagggcaagc tgtatatgtt ccagatctat aacaaggact
     3901 tttccgataa gtctcacggc acacccaatc tgcacaccat gtacttcaag ctgctgtttg
     3961 acgagaacaa tcacggacag atcaggctga gcggaggagc agagctgttc atgaggcgcg
     4021 cctccctgaa gaaggaggag ctggtggtgc acccagccaa ctcccctatc gccaacaaga
     4081 atccagataa tcccaagaaa accacaaccc tgtcctacga cgtgtataag gataagaggt
     4141 tttctgagga ccagtacgag ctgcacatcc caatcgccat caataagtgc cccaagaaca
     4201 tcttcaagat caatacagag gtgcgcgtgc tgctgaagca cgacgataac ccctatgtga
     4261 tcggcatcgc caggggcgag cgcaatctgc tgtatatcgt ggtggtggac ggcaagggca
     4321 acatcgtgga gcagtattcc ctgaacgaga tcatcaacaa cttcaacggc atcaggatca
     4381 agacagatta ccactctctg ctggacaaga aggagaagga gaggttcgag gcccgccaga
     4441 actggacctc catcgagaat atcaaggagc tgaaggccgg ctatatctct caggtggtgc
     4501 acaagatctg cgagctggtg gagaagtacg atgccgtgat cgccctggag gacctgaact
     4561 ctggctttaa gaatagccgc gtgaaggtgg agaagcaggt gtatcagaag ttcgagaaga
     4621 tgctgatcga taagctgaac tacatggtgg acaagaagtc taatccttgt gcaacaggcg
     4681 gcgccctgaa gggctatcag atcaccaata agttcgagag ctttaagtcc atgtctaccc
     4741 agaacggctt catcttttac atccctgcct ggctgacatc caagatcgat ccatctaccg
     4801 gctttgtgaa cctgctgaaa accaagtata ccagcatcgc cgattccaag aagttcatca
     4861 gctcctttga caggatcatg tacgtgcccg aggaggatct gttcgagttt gccctggact
     4921 ataagaactt ctctcgcaca gacgccgatt acatcaagaa gtggaagctg tactcctacg
     4981 gcaaccggat cagaatcttc cggaatccta agaagaacaa cgtgttcgac tgggaggagg
     5041 tgtgcctgac cagcgcctat aaggagctgt tcaacaagta cggcatcaat tatcagcagg
     5101 gcgatatcag agccctgctg tgcgagcagt ccgacaaggc cttctactct agctttatgg
     5161 ccctgatgag cctgatgctg cagatgcgga acagcatcac aggccgcacc gacgtggatt
     5221 ttctgatcag ccctgtgaag aactccgacg gcatcttcta cgatagccgg aactatgagg
     5281 cccaggagaa tgccatcctg ccaaagaacg ccgacgccaa tggcgcctat aacatcgcca
     5341 gaaaggtgct gtgggccatc ggccagttca agaaggccga ggacgagaag ctggataagg
     5401 tgaagatcgc catctctaac aaggagtggc tggagtacgc ccagaccagc gtgaagcaca
     5461 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga tcctacccat
     5521 acgatgttcc agattacgct tatccctacg acgtgcctga ttatgcatac ccatatgatg
     5581 tccccgacta tgcctaaagc ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg
     5641 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     5701 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     5761 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     5821 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     5881 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     5941 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     6001 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     6061 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     6121 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     6181 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6241 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6301 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
     6361 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6421 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     6481 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
     6541 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6601 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6661 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6721 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6781 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6841 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6901 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6961 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7021 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7081 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7141 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7201 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7261 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7321 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7381 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7441 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7501 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7561 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7621 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7681 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7741 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7801 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7861 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7921 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7981 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8041 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8101 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8161 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8221 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8281 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8341 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8401 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8461 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8521 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8581 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8641 tttccccgaa aagtgccacc tgg
//
