LOCUS       sequence-260087-        8693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for catalytically inactive Cpf1 from 
            Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman
            JS, Joung JK
  TITLE     Inducible and multiplex gene regulation using CRISPR-Cpf1-based 
            transcription factors.
  JOURNAL   Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
  PUBMED    29083402
REFERENCE   2  (bases 1 to 8693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483."
COMMENT     Sequence Label: JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65
FEATURES             Location/Qualifiers
     source          1..8693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..352
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     414..433
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             505..4188
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4189..4236
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4243..4323
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             4759..5130
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     primer_bind     complement(5217..5236)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5282..5337
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5336..5355)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5698..5714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5698..5714)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5711..5733)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5722..5738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5746..5752,5753..5770,5771..5776))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5791..5812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5871..6066
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5917..6052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6072..6206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6109..6128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6163..6182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6274..6291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6445..7033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6525..6544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7204..7995,7996..8064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7827..7846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8065..8169)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8200..8579
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
       61 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
      121 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      181 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
      241 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
      301 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
      361 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
      421 gctggttatt gtgctgtctc atcattttgg caaagaattc tgcagtcgac ggtaccgcgg
      481 gcccgggatc caccggtcgc caccatgagc aagctggaga agtttacaaa ctgctactcc
      541 ctgtctaaga ccctgaggtt caaggccatc cctgtgggca agacccagga gaacatcgac
      601 aataagcggc tgctggtgga ggacgagaag agagccgagg attataaggg cgtgaagaag
      661 ctgctggatc gctactatct gtcttttatc aacgacgtgc tgcacagcat caagctgaag
      721 aatctgaaca attacatcag cctgttccgg aagaaaacca gaaccgagaa ggagaataag
      781 gagctggaga acctggagat caatctgcgg aaggagatcg ccaaggcctt caagggcaac
      841 gagggctaca agtccctgtt taagaaggat atcatcgaga caatcctgcc agagttcctg
      901 gacgataagg acgagatcgc cctggtgaac agcttcaatg gctttaccac agccttcacc
      961 ggcttctttg ataacagaga gaatatgttt tccgaggagg ccaagagcac atccatcgcc
     1021 ttcaggtgta tcaacgagaa tctgacccgc tacatctcta atatggacat cttcgagaag
     1081 gtggacgcca tctttgataa gcacgaggtg caggagatca aggagaagat cctgaacagc
     1141 gactatgatg tggaggattt ctttgagggc gagttcttta actttgtgct gacacaggag
     1201 ggcatcgacg tgtataacgc catcatcggc ggcttcgtga ccgagagcgg cgagaagatc
     1261 aagggcctga acgagtacat caacctgtat aatcagaaaa ccaagcagaa gctgcctaag
     1321 tttaagccac tgtataagca ggtgctgagc gatcgggagt ctctgagctt ctacggcgag
     1381 ggctatacat ccgatgagga ggtgctggag gtgtttagaa acaccctgaa caagaacagc
     1441 gagatcttca gctccatcaa gaagctggag aagctgttca agaattttga cgagtactct
     1501 agcgccggca tctttgtgaa gaacggcccc gccatcagca caatctccaa ggatatcttc
     1561 ggcgagtgga acgtgatccg ggacaagtgg aatgccgagt atgacgatat ccacctgaag
     1621 aagaaggccg tggtgaccga gaagtacgag gacgatcgga gaaagtcctt caagaagatc
     1681 ggctcctttt ctctggagca gctgcaggag tacgccgacg ccgatctgtc tgtggtggag
     1741 aagctgaagg agatcatcat ccagaaggtg gatgagatct acaaggtgta tggctcctct
     1801 gagaagctgt tcgacgccga ttttgtgctg gagaagagcc tgaagaagaa cgacgccgtg
     1861 gtggccatca tgaaggacct gctggattct gtgaagagct tcgagaatta catcaaggcc
     1921 ttctttggcg agggcaagga gacaaacagg gacgagtcct tctatggcga ttttgtgctg
     1981 gcctacgaca tcctgctgaa ggtggaccac atctacgatg ccatccgcaa ttatgtgacc
     2041 cagaagccct actctaagga taagttcaag ctgtattttc agaaccctca gttcatgggc
     2101 ggctgggaca aggataagga gacagactat cgggccacca tcctgagata cggctccaag
     2161 tactatctgg ccatcatgga taagaagtac gccaagtgcc tgcagaagat cgacaaggac
     2221 gatgtgaacg gcaattacga gaagatcaac tataagctgc tgcccggccc taataagatg
     2281 ctgccaaagg tgttcttttc taagaagtgg atggcctact ataaccccag cgaggacatc
     2341 cagaagatct acaagaatgg cacattcaag aagggcgata tgtttaacct gaatgactgt
     2401 cacaagctga tcgacttctt taaggatagc atctcccggt atccaaagtg gtccaatgcc
     2461 tacgatttca acttttctga gacagagaag tataaggaca tcgccggctt ttacagagag
     2521 gtggaggagc agggctataa ggtgagcttc gagtctgcca gcaagaagga ggtggataag
     2581 ctggtggagg agggcaagct gtatatgttc cagatctata acaaggactt ttccgataag
     2641 tctcacggca cacccaatct gcacaccatg tacttcaagc tgctgtttga cgagaacaat
     2701 cacggacaga tcaggctgag cggaggagca gagctgttca tgaggcgcgc ctccctgaag
     2761 aaggaggagc tggtggtgca cccagccaac tcccctatcg ccaacaagaa tccagataat
     2821 cccaagaaaa ccacaaccct gtcctacgac gtgtataagg ataagaggtt ttctgaggac
     2881 cagtacgagc tgcacatccc aatcgccatc aataagtgcc ccaagaacat cttcaagatc
     2941 aatacagagg tgcgcgtgct gctgaagcac gacgataacc cctatgtgat cggcatcgcc
     3001 aggggcgagc gcaatctgct gtatatcgtg gtggtggacg gcaagggcaa catcgtggag
     3061 cagtattccc tgaacgagat catcaacaac ttcaacggca tcaggatcaa gacagattac
     3121 cactctctgc tggacaagaa ggagaaggag aggttcgagg cccgccagaa ctggacctcc
     3181 atcgagaata tcaaggagct gaaggccggc tatatctctc aggtggtgca caagatctgc
     3241 gagctggtgg agaagtacga tgccgtgatc gccctggagg acctgaactc tggctttaag
     3301 aatagccgcg tgaaggtgga gaagcaggtg tatcagaagt tcgagaagat gctgatcgat
     3361 aagctgaact acatggtgga caagaagtct aatccttgtg caacaggcgg cgccctgaag
     3421 ggctatcaga tcaccaataa gttcgagagc tttaagtcca tgtctaccca gaacggcttc
     3481 atcttttaca tccctgcctg gctgacatcc aagatcgatc catctaccgg ctttgtgaac
     3541 ctgctgaaaa ccaagtatac cagcatcgcc gattccaaga agttcatcag ctcctttgac
     3601 aggatcatgt acgtgcccga ggaggatctg ttcgagtttg ccctggacta taagaacttc
     3661 tctcgcacag acgccgatta catcaagaag tggaagctgt actcctacgg caaccggatc
     3721 agaatcttcc ggaatcctaa gaagaacaac gtgttcgact gggaggaggt gtgcctgacc
     3781 agcgcctata aggagctgtt caacaagtac ggcatcaatt atcagcaggg cgatatcaga
     3841 gccctgctgt gcgagcagtc cgacaaggcc ttctactcta gctttatggc cctgatgagc
     3901 ctgatgctgc agatgcggaa cagcatcaca ggccgcaccg acgtggattt tctgatcagc
     3961 cctgtgaaga actccgacgg catcttctac gatagccgga actatgaggc ccaggagaat
     4021 gccatcctgc caaagaacgc cgacgccaat ggcgcctata acatcgccag aaaggtgctg
     4081 tgggccatcg gccagttcaa gaaggccgag gacgagaagc tggataaggt gaagatcgcc
     4141 atctctaaca aggagtggct ggagtacgcc cagaccagcg tgaagcacaa aaggccggcg
     4201 gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat cctacccata cgatgttcca
     4261 gattacgctt atccctacga cgtgcctgat tatgcatacc catatgatgt ccccgactat
     4321 gccggaagcg agttccagta cctgccagat acagacgatc gtcaccggat tgaggagaaa
     4381 cgtaaaagga catatgagac cttcaagagc atcatgaaga agagtccttt cagcggaccc
     4441 accgaccccc ggcctccacc tcgacgcatt gctgtgcctt cccgcagctc agcttctgtc
     4501 cccaagccag caccccagcc ctatcccttt acgtcatccc tgagcaccat caactatgat
     4561 gagtttccca ccatggtgtt tccttctggg cagatcagcc aggcctcggc cttggccccg
     4621 gcccctcccc aagtcctgcc ccaggctcca gcccctgccc ctgctccagc catggtatca
     4681 gctctggccc aggccccagc ccctgtccca gtcctagccc caggccctcc tcaggctgtg
     4741 gccccacctg cccccaagcc cacccaggct ggggaaggaa cgctgtcaga ggccctgctg
     4801 cagctgcagt ttgatgatga agacctgggg gccttgcttg gcaacagcac agacccagct
     4861 gtgttcacag acctggcatc cgtcgataac tccgagtttc agcagctgct gaaccagggc
     4921 atacctgtgg ccccccacac aactgagccc atgctgatgg agtaccctga ggctataact
     4981 cgcctagtga caggggccca gaggcccccc gacccagctc ctgctccact gggggccccg
     5041 gggctcccca atggcctcct ttcaggagat gaagacttct cctccattgc ggacatggac
     5101 ttctcagccc tgctgagtca gatcagctct taaggccgca ctcctcaggt gcaggctgcc
     5161 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     5221 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     5281 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     5341 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     5401 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     5461 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     5521 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     5581 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     5641 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     5701 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5761 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5821 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     5881 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5941 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6001 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     6061 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6121 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6181 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     6241 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7081 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7141 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7201 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7261 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7321 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7381 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7441 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7501 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7561 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7621 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7681 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7741 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7801 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7861 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7921 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7981 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8041 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8101 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8161 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt
     8221 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     8281 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     8341 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     8401 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     8461 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     8521 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     8581 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     8641 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg ggg
//
