LOCUS       sequence-260092-        8880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpress EWS-FLI1. 3rd generation lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Loganathan SN, Tang N, Fleming JT, Ma Y, Guo Y, Borinstein SC, 
            Chiang C, Wang J
  TITLE     BET bromodomain inhibitors suppress EWS-FLI1-dependent transcription
            and the IGF1 autocrine mechanism in Ewing sarcoma.
  JOURNAL   Oncotarget. 2016 Jul 12;7(28):43504-43517. doi: 
            10.18632/oncotarget.9762.
  PUBMED    27259270
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget"; journalName: "Oncotarget"; date: "2016-07-12-
            12"; volume: "7"; issue: "28"; pages: "43504-43517"
COMMENT     Sequence Label: pCDH-puro-EWS-FLI1
FEATURES             Location/Qualifiers
     source          1..8880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    66..187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(77..96)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     131..150
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             259..492
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    566..1154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             672..683
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1081..1101
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1161..1760)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1244..1264)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1741..1760
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     LTR             1787..2055
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(2068..2279)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(3876..3900)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3897..4100)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3926..3946)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    4190..4306
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4573..4617)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4802..5035
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5528..5653
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5700..5880
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5881..6107)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6122..6139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6122..6138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6131..6153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6347..6366)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6466..6488
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6526..6544)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6612..6716
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6717..6785,6786..7577)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6935..6954)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7748..8336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8490..8507
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8624..8645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8660..8665,8666..8683,8684..8690)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8698..8714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8703..8725
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8722..8738
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8722..8738
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(8771..8880,1..26)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggatagc tagagccaga
       61 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      121 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      181 acaagttcct ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa
      241 gatgacatga actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc
      301 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
      361 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
      421 ggtctaacca gagagaccca gtacaagcaa aaagcagatc ttatcttcgt tgggagtgaa
      481 ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
      541 aagtcattgg tcttaaaggt accaggcggg gaggcggccc aaagggagat ccgactcgtc
      601 tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga
      661 aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag
      721 gtggcaacac aggcgagcag ccaaggaaag gacgatgatt tccccgacaa caccacggaa
      781 ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt
      841 tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg
      901 gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc
      961 ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag
     1021 aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca
     1081 catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa
     1141 tccagaggtt gattgtcgac tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt
     1201 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg
     1261 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact
     1321 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg
     1381 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt
     1441 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac
     1501 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc
     1561 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg
     1621 gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg
     1681 aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc
     1741 gtgggcttgt actcggtcat ctagcgtagg cgccggtcac agcttggatc tgtaacggcg
     1801 cagaacagaa aacgaaacaa agacgtagag ttgagcaagc agggtcaggc aaagcgtgga
     1861 gagccggctg agtctaggta ggctccaagg gagcgccgga caaaggcccg gtctcgacct
     1921 gagctttaaa cttacctaga cggcggacgc agttcaggag gcaccacagg cgggaggcgg
     1981 cagaacgcga ctcaaccggc gtggatggcg gcctcaggta gggcggcggg cgcgtgaagg
     2041 agagatgcga gcccctcgaa gcttcagctg tgttctggcg gcaaacccgt tgcgaaaaag
     2101 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     2161 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcacccgt
     2221 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca
     2281 ctgacgggca ccggagcgat cgcagatcct tcgcggccgc ctagtagtag ctgcctaagt
     2341 gtgaaggcac gtgggtgtta ggatggcggg ggacgttggg gttggggtag attccccccg
     2401 tgggggaggt ccagtattgt gatgcggctc caaagaagct ggaggaagtg acaggcatgg
     2461 aggatggatg gggagggaca aagttcacct tctgctggtg ggcatggtag gaaggcatgt
     2521 aggagatgtc agaagggtac ttgtacatgg acgactcggt cggatgtggc tgcagagcct
     2581 gggcaatgcc gtggaagtca aatttgtaag catatctttt gccgtgcact ttggtcataa
     2641 tgtttttatc atagtaataa cggagggccc ggctcagctt gtcgtaattc atgttgggct
     2701 tgcttttccg ctcgccccag cgcctggcca cctcatcggg gtccgtcatt ttgaactccc
     2761 cgttggtccc ctcccaggtg atacagctgg cgttggcgct gtcggagagc agctccagga
     2821 ggaattgcca cagctggatc tgcccgcttc cagggttggc taggcgactg ctggtcgggc
     2881 ccaggatctg atacggatct ggctggggcc gttgctctgt attcttactg atcgtttgtg
     2941 cccctccaag gggaggactt ttgttgaggc cagaattcat gttattgccc catgctcctc
     3001 ttctgactga gtcataagaa gggttctgct gcccgtagct gctgctctgt tggctatatt
     3061 gacttggagc ttggctgtag gatccagttt ggggtgggta actagtggga ggctgctgcc
     3121 catagctgct ttgttgacca tagctactct gctgtccata gctgctcggt tgcccatagg
     3181 tgttctgctg agagtaactg ctctgatcat aactagtcgg ctgtgtagag gaatagctgg
     3241 taggagggta ggatggaggt gcagtgactg gctgcatggg gtagctccca ggtacctggg
     3301 gataactgta gttactctgt ccatatccta ggctgggctg gttgtaaccc cctgtgctag
     3361 attgaggttg actagtctca gtgggcttgt ttccatcctg cggtcttgta ggtgcagtgg
     3421 ctgctggctg ctgcccatag gctggataag caggctgagt gccatatgca gactgagctg
     3481 cataggaggc ctgggtggtg gtgactgtag cagtggtggt atcataagca ccagtgccat
     3541 acccctggac aggctggctg tatgcctggg gggcagttgg agtagtataa ccagtgggag
     3601 gctgtccata agaagttgca taggcggtct gcccataggt tgcagtggtc tgagcctggg
     3661 tatagctgac atcagtgggc tgtccatagg ttccatagct ttgttgccca tatgcctggg
     3721 tggtctgtgc atatccttga gtgggctggg cggtgtaagc actgtagccc tgctgcgctg
     3781 cagcttggct ataggtactg taatccgtgg acgccatggt gctagctcta gaatcttcta
     3841 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
     3901 ctgcttatat aaacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
     3961 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
     4021 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
     4081 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
     4141 gtaggaaagt cccataaggt catgtactgg gcataatact agtatcgata aaattttgaa
     4201 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     4261 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaagtta accgataccg
     4321 tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc
     4381 tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
     4441 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
     4501 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
     4561 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
     4621 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
     4681 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
     4741 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
     4801 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
     4861 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
     4921 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
     4981 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
     5041 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
     5101 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
     5161 tcctcctcca ggtctgaaga tcagtggccg cttgctgtgc ggtggtctta cttttgtttt
     5221 gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca
     5281 cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa
     5341 gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag
     5401 gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt
     5461 ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct
     5521 taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg
     5581 gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc
     5641 tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact
     5701 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
     5761 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
     5821 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
     5881 gtttatgtat cgagctaggc acttaaatac aatatctctg caatgcggca attcagtggt
     5941 tcgtccaatc catgtcagac ccgtctgttg ccttcctaat aaggcacgat cgtaccacct
     6001 tacttccacc aatcggcatg cacggtgctt tttctctcct tgtaaggcat gttgctaact
     6061 catcgttacc atgttgcaag actacaagag tattgcataa gactacacgc gtcagcttgg
     6121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6241 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     6301 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     6361 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6421 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6481 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     6541 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     6601 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6661 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6721 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6781 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6841 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6901 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6961 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7021 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7081 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7141 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7201 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7261 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7321 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7381 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7441 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7501 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7561 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7621 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7681 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7741 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7801 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7861 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7921 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7981 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8041 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8101 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8161 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8221 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8281 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8341 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8401 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8461 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8521 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8581 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8641 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8701 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttggtctctg
     8761 caaaagtcta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     8821 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
//
