LOCUS       sequence-260096-        2607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TRPML1 with C-terminus mCherry tag in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ruas M, Davis LC, Chen CC, Morgan AJ, Chuang KT, Walseth TF, Grimm 
            C, Garnham C, Powell T, Platt N, Platt FM, Biel M, Wahl-Schott C, 
            Parrington J, Galione A
  TITLE     Expression of Ca(2)(+)-permeable two-pore channels rescues NAADP 
            signalling in TPC-deficient cells.
  JOURNAL   EMBO J. 2015 Jul 2;34(13):1743-58. doi: 10.15252/embj.201490009. 
            Epub 2015 Apr 14.
  PUBMED    25872774
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201490009"; journalName: "EMBO J"; date: "2015-07-2- 
            2"; volume: "34"; issue: "13"; pages: "1743-58"
COMMENT     Sequence Label: TRPML1-mCherry
FEATURES             Location/Qualifiers
     source          1..2607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..768)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNLAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYKL"
     primer_bind     complement(339..358)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     608..626
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctagcgcatg ctccngactg ccttgggaaa agcgcctccc ctacccggta gaattatcta
       61 gagcttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
      121 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
      181 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
      241 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
      301 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
      361 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
      421 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
      481 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
      541 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
      601 gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
      661 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
      721 catgaactcc ttgatgatgg ctaagttatc ctcctcgccc ttgctcaccg cggccgcgtt
      781 caccagcagc gaatggtcct ccggggagtc ccttccacag cagcagaaaa ggctacaagc
      841 tgagccactg ccacgacgga acttgccaga tgtggggcta tcctggcact gctcgatgta
      901 ggcctggagc tcactcttct ctgtgccagt acctcctggg tgcttgatgg tgtcataggc
      961 gccggtgatg agtgcaatga agaggctcag caccatgtag atgaagaggc tgatgaagga
     1021 gtagaggtac agctgggaga agagccacac caggctgctg tgaccctgct gggcctgcat
     1081 ggccgcgaac gtcacaaaca tgtcgtctcc gttgatgagt gagaacagac actcagaaac
     1141 catggacagc gagcggaact tcacatggta gggccctaga acgatccagc cacagaagca
     1201 atagcccagg tagatgacag ccacacagca gcagaaacgc atgacactgg gcagtgccac
     1261 tcgcaacgtg gcaatcaaga tgttgtactt gtggaaaaat gtcaggtagc gaatgacacc
     1321 gacccagact agcagagtgg aggtacccaa gagaatactg cagacatcat agctggctag
     1381 gttctttgcc tcaatgccaa tcttcatgac agtccccgag atggtgagca cgtcactggt
     1441 gaccagcagg atgtaccagc cattgacaaa ctccagccgt tcccagaggc tgatttcccg
     1501 accccgccgc cgccacatga atacaacaaa ctcgttctgc agcaggaagc cacggagcag
     1561 tgagcgggcg cacagcagga aggacaggga gcaggtgagg ataaccacca catcaaacag
     1621 aagccggaag ctgttgtctc catgtctgga gacactgggg tgtttgcact cctggatgtg
     1681 ggtcttggtc tccaggcgga tggggattcg cccactgtgc gctttattgt caaatgtgat
     1741 caggatactg aaggtgtaac aatcagggat ctcattgttg atgaggctct gcaggttaat
     1801 tgtcttcagc tggaagtgga tggtgacgtt gatcagcttg tggaatttca gtgtgaggtt
     1861 cttgtaactg gcgctgccat ccaagaagtc caagtcctca ctggggatgt cagggggtct
     1921 gtcaggagga tccacctgga tacagtcagt gactaccctt ggatcaatgt caaaggtatc
     1981 attggctggg tccacatggc cacggtggta gtaccgctgg cagagagcca aagctgatcc
     2041 attggcccaa ggcccacccc caccacggac ataggcatac cggcccaggg atatctcagg
     2101 tagtatcagg tactggtcca cagcatagaa gatggcttgg tagagctgct cctgtgtgta
     2161 ggctgcaaag gtgtcatcag acccatcaga gtaacccagc aggaagagat gtcggaaggc
     2221 aatggtgttc tcttcccgga atgtcaccac cagctggttg ctgagcccaa agagaatgag
     2281 ctgcacagtg accaccaaga tcttgaccac ctgcagcatc agcttgcagg gcttgcggcc
     2341 tttggcccgg aacttgtcac atgggctcat aaagaagtac ttgaggcggc ggcggaggtc
     2401 ttcctcttct gtgggggttg ttggggctgg tgaggtcccc acctgggtcc catacccagg
     2461 attgggggtc agcagccgct cagtctctga ggcgcgccgg cccgccgggg tggccatggt
     2521 gaattcgaag cttgagctcg agatctgagt ccggtagcgc tagcgatcct ctagtagagt
     2581 cggtgtcttc tanggaggtc aaaacag
//
