LOCUS       sequence-260099-        4230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC17A7
FEATURES             Location/Qualifiers
     source          1..4230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2355..2454)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2471..2490)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2472..2490)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2495..2511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2495..2511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2613..2632)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2624..3433
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2691..2710)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3580..4168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4069..4088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggagt tccggcagga ggagtttaga aagctggcag gaagggccct
      721 gggcaagctg cacaggctgc tggagaagag gcaggaggga gccgagaccc tggagctgag
      781 cgccgacggc cgccctgtga ccacacagac cagggaccca cctgtggtgg attgcacatg
      841 tttcggcctg ccacggagat acatcatcgc catcatgtcc ggcctgggct tctgcatctc
      901 ttttggcatc agatgtaacc tgggcgtggc catcgtgtcc atggtgaaca attctaccac
      961 acacagggga ggacacgtgg tggtgcagaa ggcacagttc tcctgggatc ccgagaccgt
     1021 gggcctgatc cacggctctt tcttttgggg ctatatcgtg acacagatcc ctggcggctt
     1081 catctgccag aagtttgccg ccaaccgggt gttcggcttt gccatcgtgg ccaccagcac
     1141 actgaatatg ctgatcccat ccgccgcaag agtgcactac ggatgcgtga tcttcgtgag
     1201 gatcctgcag ggcctggtgg agggcgtgac ctatcctgcc tgtcacggca tctggtctaa
     1261 gtgggcacca ccactggaga ggagcaggct ggccaccaca gccttttgtg gcagctacgc
     1321 aggagcagtg gtggcaatgc cactggccgg cgtgctggtg cagtattctg gctggagctc
     1381 cgtgttctac gtgtatggca gcttcggcat cttttggtac ctgttttggc tgctggtgag
     1441 ctatgagtcc ccagccctgc acccctctat cagcgaggag gagagaaagt acatcgagga
     1501 cgccatcggc gagtccgcca agctgatgaa ccccctgacc aagttctcca caccttggag
     1561 gcgcttcttt acctctatgc ccgtgtacgc catcatcgtg gccaatttct gcaggagctg
     1621 gaccttctac ctgctgctga tctcccagcc agcctatttt gaggaggtgt tcggctttga
     1681 gatctccaaa gtgggcctgg tgtctgccct gccacacctg gtcatgacca tcatcgtgcc
     1741 catcggagga cagatcgcag acttcctgcg cagccggaga atcatgtcca ccacaaacgt
     1801 gcggaagctg atgaattgtg gcggctttgg catggaggcc acactgctgc tggtggtggg
     1861 ctactcccac tctaagggcg tggccatcag cttcctggtg ctggccgtgg gcttctctgg
     1921 ctttgccatc agcggcttta acgtgaatca cctggatatc gccccacgct atgccagcat
     1981 cctgatgggc atctccaatg gcgtgggcac cctgtctggc atggtgtgcc ccatcatcgt
     2041 gggcgccatg accaagcaca agacacggga ggagtggcag tacgtgttcc tgatcgcctc
     2101 cctggtgcac tacggcggcg tgatcttcta tggcgtgttt gcaagcggag agaagcagcc
     2161 ttgggcagag ccagaggaga tgtccgagga gaagtgtgga tttgtgggac acgaccagct
     2221 ggcaggatct gacgatagcg agatggagga tgaggcagag cctccaggag caccacctgc
     2281 accaccacct tcttacggag caacccacag cacattccag ccacccagac ctccaccccc
     2341 tgtgagggat tatgacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2401 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2461 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2521 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     2581 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2641 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     2701 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     2761 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     2821 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     2881 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     2941 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3001 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3061 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3121 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3181 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3241 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3301 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3361 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3421 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     3481 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3541 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3601 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3661 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3721 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3781 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3841 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3901 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3961 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4021 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4081 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4141 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4201 ggccttttgc tggccttttg ctcacatgtt
//
