LOCUS       sequence-260122-        4324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Macrophage specific label.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Oehlers SH, Cronan MR, Scott NR, Thomas MI, Okuda KS, Walton EM, 
            Beerman RW, Crosier PS, Tobin DM
  TITLE     Interception of host angiogenic signalling limits mycobacterial 
            growth.
  JOURNAL   Nature. 2015 Jan 29;517(7536):612-5. doi: 10.1038/nature13967. Epub 
            2014 Nov 24.
  PUBMED    25470057
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13967"; journalName: "Nature"; date: "2015-01-29- 
            29"; volume: "517"; issue: "7536"; pages: "612-5"
COMMENT     Sequence Label: p5E mpeg1
FEATURES             Location/Qualifiers
     source          1..4324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(59..145)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     194..216
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     208..225
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     209..225
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    265..360
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        362..380
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2298..2314)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2298..2314)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2351..2369)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2370..2493
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2566..2584)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2589..2605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2589..2605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2707..2726)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2718..3527
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2785..2804)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3674..4262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4163..4182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
       61 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      121 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
      181 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
      241 gcgttaacgc taccatggag ctccaaataa tgattttatt ttgactgata gtgacctgtt
      301 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtat agaaaagttg
      361 gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagt tgttggagca
      421 catctgacat ctgaaattat ttgaaagcag cagaaatgtt tttacagcta ttacaaaatt
      481 tacttaacaa gaatccttta aaatatgaca atttattgcc aataatttac agtaagtttt
      541 gcagtacttt aatattacaa attatacaac tgtgctggca cacaaaaaat aacttaatcc
      601 agcacaacaa caacaacaac aactacaaaa agcatatttc cttttgcttt ttcttgttca
      661 ctcaactttt ggaaacatca actgcactga cctaaaatta tacagtatgt cagtaataca
      721 aaaacactta gttaggttaa cataagatta ttagttttct ggagaggtga actactctgt
      781 aagaaacctt tttaagttgt gccaactaaa catttttatc agcagaacta caatgcttga
      841 tgagtgaaca aaaaaacaaa caaaccttat ttccttttgt tttttcttgt gtacttaaca
      901 tttttttcac ctttatttga aacgacagta gggagtatgg acaggaaagt gttgggagag
      961 gagagggatg agcatagaac caggaggtag gaatcaaact caggtcacta tgaacaccgg
     1021 agtgccaggt gtcaacacac taaccactac accatcaact ccaacattta cttgaaaaca
     1081 tcagttgagt gaacaaaaaa ctcttgtgga aattggtact gagaaataat acattatcac
     1141 aacaaacaaa acagagacaa atgttaacaa aacaattatt agaaacagaa ttagaaaccg
     1201 gacaggaggt aaaacaataa tgataaacaa gacaaggatc aaaacatggc aggacaaaga
     1261 cagttttttg ttcactcaac ctcagatatt ttaggtcagt gcaactgatg tttacaaagg
     1321 ttgtatgcat gtcaacacca atggtgtagt gattagtgtg ttgacacaag gcactccagt
     1381 gttcacactg atgctggttt gattcccaca tcgatgtcct ttgctaatcc tgcctctctc
     1441 cccatgcttt cctgtctata ctctctactg ttgtgtcaat taaaggtgaa aaaagttaag
     1501 cacaaaagaa aaatctaagg atataaggat tatgtttttt tttgttcact caaaatcagg
     1561 cattccagtt ctgcagacaa aaaatgttca gttggcacaa cttaaaaagt tttgttacag
     1621 agtagttcac ctctccagaa aactaataat cttatgttaa cccaactaaa tgtttttact
     1681 gaaaaataat tgaatgtcag tgcagctgat gtttttttaa aagtggagag aacaaaaaaa
     1741 gtgaaaggaa ataaggattc attgttgtac cgacttattt ttttgaagaa aattctgcta
     1801 tagggctttc atgatcccct atgctcacag cagttttagg agatgcttat aataaagagg
     1861 atttgaaaaa cctgctaatc ttgtacattt ctaaaacaag aagccaacca atttcaattc
     1921 ctcctctttc aaagccaatg gaaaatttaa aacgcacagt ctcttgcgtc atcaaaacca
     1981 ttctgaagac tcatcaaaat ccttcgtagc tttaaatata aatctattat tgaacagatc
     2041 atacttcaca aaaaatataa atatatttga ttcattcact tcatgaaatt gtccttcaac
     2101 ttaacgtttg aacgcaaggt accaaaacac aaagaggaac ctcatagtct catgaccctt
     2161 cccccacgga tttgaaactg agaagtggtt ttattgagga actcagcaaa atctataatc
     2221 accatatttg attgaaaatc acattagtgc aggagacagc aaaacaaagc ttgatatcga
     2281 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
     2341 gcttttgttc cctttagtga gggttaattc aagtttgtac aaaaaagttg aacgagaaac
     2401 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     2461 atactgtaaa acacaacata tgcagtcact atgaaccaac tacttagatg gtattagtga
     2521 cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt
     2581 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     2641 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     2701 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     2761 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     2821 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     2881 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     2941 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     3001 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     3061 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     3121 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     3181 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     3241 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     3301 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     3361 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     3421 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     3481 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     3541 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     3601 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     3661 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3721 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3781 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3841 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3901 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3961 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4021 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     4081 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4141 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4201 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4261 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4321 tgtt
//
