LOCUS       sequence-260140-        3045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dominate Negative zebrafish e-cadherin label.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Cronan MR, Beerman RW, Rosenberg AF, Saelens JW, Johnson MG, Oehlers
            SH, Sisk DM, Jurcic Smith KL, Medvitz NA, Miller SE, Trinh LA, 
            Fraser SE, Madden JF, Turner J, Stout JE, Lee S, Tobin DM
  TITLE     Macrophage Epithelial Reprogramming Underlies Mycobacterial 
            Granuloma Formation and Promotes Infection.
  JOURNAL   Immunity. 2016 Oct 18;45(4):861-876. doi: 
            10.1016/j.immuni.2016.09.014.
  PUBMED    27760340
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2016.09"; journalName: "Immunity"; date: 
            "2016-10-18- 18"; volume: "45"; issue: "4"; pages: "861-876"
COMMENT     Sequence Label: pME DN-E-cadherin
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(389..475)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     524..546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     538..555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     539..555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    571..670
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             676..717
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     protein_bind    complement(1172..1271)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1288..1307)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1289..1307)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1312..1328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1312..1328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1430..1449)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1441..2250
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1508..1527)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2397..2985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2886..2905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
      241 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      301 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
      361 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
      421 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
      481 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
      541 aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg
      601 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      661 aaagcaggct ggaccatggg aagctctaag agcaagccta aagacccaag ccagagaaga
      721 cggaggaaaa gcaactccaa aaaagaaccg ctccttccag aagatgatgt acgggacaac
      781 atctactatt atgatgagga aggcggtgga gaagatgatc aggactttga tttgagtgtc
      841 ctgcacagag gcctggacaa cagaccagaa gtgttcagga atgatgtggc gcccactttc
      901 atgcctgcac ctcagtacag acctcgaccg gccaaccctg aggagatcgg cacattcatt
      961 gatgataacc tgaaggctgc agataacgac cccacagctc ctccctacga ctctctgctg
     1021 gtgtttgact atgaaggagg aggatcagac gcaggttcac tcagctccct caacacttcc
     1081 agctcaggaa acgaccaaga ctatgacttc ctcaatgagt ggggcccacg cttcaagaag
     1141 ctagcggaca tgtatggagg aggagaggat aacccagctt tcttgtacaa agttggcatt
     1201 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1261 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1321 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1381 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     1441 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     1501 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     1561 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     1621 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     1681 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     1741 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     1801 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     1861 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     1921 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     1981 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     2041 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     2101 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     2161 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     2221 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     2281 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     2341 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2401 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2461 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2521 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2581 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2641 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2701 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2761 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2821 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2881 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2941 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3001 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatg
//
