LOCUS       sequence-260157-        2586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre coexpressed with tdTomato in macrophages.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Walton EM, Cronan MR, Beerman RW, Tobin DM
  TITLE     The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term 
            Analysis of Macrophage Behavior during Mycobacterial Infection in 
            Zebrafish.
  JOURNAL   PLoS One. 2015 Oct 7;10(10):e0138949. doi: 
            10.1371/journal.pone.0138949. eCollection 2015.
  PUBMED    26445458
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-7"; volume: "10(10):e0138949. doi"; pages: " 
            10.1371/journal.pone.0138949. eCollection 2015"
COMMENT     Sequence Label: pDEST mfap4:icre-p2a-tomato
FEATURES             Location/Qualifiers
     source          1..2586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1055..1079)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1096..1152
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1159..2586
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 atggtgccca agaagaagag gaaagtctcc aacctgctga ctgtgcacca aaacctgcct
       61 gccctccctg tggatgccac ctctgatgaa gtcaggaaga acctgatgga catgttcagg
      121 gacaggcagg ccttctctga acacacctgg aagatgctcc tgtctgtgtg cagatcctgg
      181 gctgcctggt gcaagctgaa caacaggaaa tggttccctg ctgaacctga ggatgtgagg
      241 gactacctcc tgtacctgca agccagaggc ctggctgtga agaccatcca acagcacctg
      301 ggccagctca acatgctgca caggagatct ggcctgcctc gcccttctga ctccaatgct
      361 gtgtccctgg tgatgaggag aatcagaaag gagaatgtgg atgctgggga gagagccaag
      421 caggccctgg cctttgaacg cactgacttt gaccaagtca gatccctgat ggagaactct
      481 gacagatgcc aggacatcag gaacctggcc ttcctgggca ttgcctacaa caccctgctg
      541 cgcattgccg aaattgccag aatcagagtg aaggacatct cccgcaccga tggtgggaga
      601 atgctgatcc acattggcag gaccaagacc ctggtgtcca cagctggtgt ggagaaggcc
      661 ctgtccctgg gggttaccaa gctggtggag agatggatct ctgtgtctgg tgtggctgat
      721 gaccccaaca actacctgtt ctgccgggtc agaaagaatg gtgtggctgc cccttctgcc
      781 acctcccaac tgtccacccg ggccctggaa gggatctttg aggccaccca ccgcctgatc
      841 tatggtgcca aggatgactc tgggcagaga tacctggcct ggtctggcca ctctgccaga
      901 gtgggtgctg ccagggacat ggccagggct ggtgtgtcca tccctgaaat catgcaggct
      961 ggtggctgga ccaatgtgaa cattgtgatg aactacatca gaaacctgga ctctgagact
     1021 ggggccatgg tgaggctgct cgaggatggg gacgacccag ctttcttgta caaagtggtt
     1081 ggatccggaa gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag
     1141 aaccctggac ctctcgagat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc
     1201 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     1261 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     1321 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     1381 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     1441 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     1501 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     1561 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     1621 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     1681 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     1741 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1801 tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc
     1861 agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc
     1921 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     1981 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     2041 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     2101 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     2161 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     2221 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     2281 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     2341 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     2401 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     2461 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     2521 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
     2581 tacaag
//
