LOCUS       sequence-260179-        8896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of MBP:PGL-1 in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.02
FEATURES             Location/Qualifiers
     source          1..8896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..917
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(843..862)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1003..1025
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1019..1210)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        1706..1783
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             1784..2866
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(1803..1822)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(3109..3114,3115..3130,3131..3137)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    3145..3161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3150..3172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             3231..4331
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     4305..4328
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             4380..4391
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    4395..4419
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6615..6639)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6690..6707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6690..6706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6699..6721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6885..6902)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7055..7141
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7233..7260
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        7279..7370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7371..7439,7440..8231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7589..7608)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8273..8786)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8468..8489)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8680..8699
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc
      841 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
      901 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      961 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1021 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     1081 ggtcgtgcag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     1141 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     1201 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     1261 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     1321 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     1381 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     1441 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     1501 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     1561 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     1621 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     1681 aggacccaac gctgcccgaa attccgacac catcgaatgg tgcaaaacct ttcgcggtat
     1741 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
     1801 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     1861 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     1921 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     1981 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     2041 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     2101 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     2161 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     2221 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     2281 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     2341 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     2401 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     2461 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     2521 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     2581 ggatatctca gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     2641 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     2701 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     2761 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2821 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2881 atgtaagtta gctcactcat taggcacaat tctcatgttt gacagcttat catcgactgc
     2941 acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag
     3001 gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt
     3061 tttgcgccga catcataacg gttctggcaa atattctgaa atgagctgtt gacaattaat
     3121 catcggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagcc
     3181 agtccgttta ggtgttttca cgagcacttc accaacaagg accatagcat atgaaaatcg
     3241 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
     3301 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
     3361 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
     3421 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
     3481 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
     3541 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
     3601 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
     3661 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
     3721 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
     3781 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac
     3841 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag
     3901 cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg
     3961 gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga
     4021 gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact
     4081 atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag
     4141 cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc actatggaaa
     4201 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg
     4261 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag
     4321 acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga
     4381 tcgagggaag gatcacaagt ttgtacaaaa aagcaggctt aatggaggct aacaagcgag
     4441 aaattgtgga tttcggtggg ctgagatcct actttttccc aaatttggcc cactacatca
     4501 cgaagaatga cgaagaactg ttcaacaata catctcaggc caataagttg gctgctttcg
     4561 tgttgggagc ttccaaagac gctcctggag acgaagacat tttggagatg atcttgccga
     4621 acgacgctaa cgctgccgtt attgctgctg gcatggacgt ttgtcttctt ctcggagata
     4681 aatttcgtcc gaaatttgat gctgctgctg agaaattgtc aggattggga catgctcatg
     4741 atctggtttc tgtcatcgat gacgacaaga agctcggaat gctcgcccgt aaggccaagc
     4801 tgaagaagac tgaagacgct aagattcttc aagctcttct caaagtaatc gcaatcgatg
     4861 atgccgccga aaagtttgtt gagctcacgg aacttgtcag ccaactggat ctggactttg
     4921 atgtttatgt cctaactaaa atcctcggac ttatctctga ggagactagc gacgaggtcg
     4981 acattatccg tgataacgtc gtcaacgcat ttgatagctg caagccgttg ttgaaacagt
     5041 tgatgttgga tggtccgaaa tctgaacctg ccgatccatt cattagtctc ctcatggatc
     5101 cactcgaaga gtctgttggg aaagttgtga accatatcgc tcaacttttc gaggaagcga
     5161 gtaaaaatga aggagacgag tcacttgtct tgagatctca actcggatat cagctctttt
     5221 tccttatcgt tcgttcactt gcagatggaa agcgcgaggt ttccaagaaa atcctctctg
     5281 ggattcctac gagtgtcaga gcggaagtct ttccaggatt gcaaagaagt gtctacaaga
     5341 gtgcggtgtt cctcggcaat cacatcatcc aagttctttt aggatctaag aagtcttttg
     5401 aggattggga tgttgttgga gtcgcgaaag acttggagag cgcctggaaa agacgagcca
     5461 ttgcggaact tatcaagaag ttccaagtct caattctaga gcaatgcttc gataagcccg
     5521 ttccactgat tcctcagagt ccactaaaca atgatgcggt gatcgataat gtgaataaag
     5581 ctctacagtt tgcactgtgg cttacagagt tttacggatc cgagaatgag actgaagcgc
     5641 tgggcgagct tcgtttcttg gattccactt caaaaaatct tctcgtcgat agcttcaaga
     5701 aatttgttca aggaatcaac tcgaagactc atgtcactcg aattgttgaa tcactggaga
     5761 agtgttgttt gtccgataca ccaagtggca gaaagagcaa tgtgcaacca tccacttccc
     5821 aacagcagga ttccgcctac acgaaagagg aaatgacgac ggttcacaac acatacagcg
     5881 taaatactaa agctcaagtt ctcaacggtc tcagtgatac caattcttcc gggttacttg
     5941 tcgattcaaa ggattcactc agtcttcagg agatttcttg tgacgaagtg gattcttcga
     6001 ctttgctaag ctcatcaaga aacattggag aaggtgttac tgtaaaagcg gtcgatcctg
     6061 tcccagaaaa ggttaacgat gcacaacaac agcaaacagt taacgagatc gagatggctt
     6121 cggatgccaa tcaagatact tcttcaagtg cttcgccaga agtagcgcca agtttttcca
     6181 ccgatggctg ggattctcca acgaaatcag tagctctgcc accgggcatg cagcagatag
     6241 atgaagagga aaccacagtt gctgacaaag attctacacc acaaccacaa gctcgtgctg
     6301 aaactgcatg ggggtcaggc gacgctactc caatgcctct tcctgccccg actaatcaat
     6361 acaaagtcag tggatttggg gaagccaaag ttgcaaaagg attcggtcaa tttgctccca
     6421 ccagttcagc ttatggagga ggaggtggtc gcggaggata tggcggtgga gaccgtggag
     6481 gcagaggtgg atacggcgga gatcgtggag gtagaggtgg atacggcggg ggagaccgcg
     6541 gaggacgagg tggatacggc ggagatcgtg gacgtggtgg ttacgggggt cgtggtggac
     6601 gcggaggttt ctaaacccag ctttcttgta caaagtggtg atatttcaga attcggatcc
     6661 tctagagtcg acctgcaggc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     6721 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6781 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6841 gaatggcagc ttggctgttt tggcggatga gataagattt tcagcctgat acagattaaa
     6901 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     6961 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     7021 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     7081 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     7141 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     7201 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     7261 tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7321 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7381 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7441 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7501 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7561 tcccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     7621 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7681 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7741 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7801 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7861 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7921 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7981 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8041 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8101 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8161 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8221 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaccccggt
     8281 tgataatcag aaaagcccca aaaacaggaa gattgtataa gcaaatattt aaattgtaaa
     8341 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     8401 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     8461 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     8521 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt
     8581 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     8641 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     8701 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     8761 gcttaatgcg ccgctacagg gcgcgtaaaa ggatctaggt gaagatcctt tttgataatc
     8821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8881 agatcaaagg atcttc
//
