LOCUS       sequence-260181-        8530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of MBP:PAA-1 in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.15
FEATURES             Location/Qualifiers
     source          1..8530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..917
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(843..862)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1003..1025
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1019..1210)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        1706..1783
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             1784..2866
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(1803..1822)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(3109..3114,3115..3130,3131..3137)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    3145..3161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3150..3172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             3231..4331
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     4305..4328
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             4380..4391
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    4395..4419
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6249..6273)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6324..6341)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6324..6340)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6333..6355)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6519..6536)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6689..6775
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6867..6894
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6913..7004
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7005..7073,7074..7865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7223..7242)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7907..8420)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8102..8123)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8314..8333
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc
      841 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
      901 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      961 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1021 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     1081 ggtcgtgcag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     1141 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     1201 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     1261 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     1321 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     1381 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     1441 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     1501 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     1561 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     1621 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     1681 aggacccaac gctgcccgaa attccgacac catcgaatgg tgcaaaacct ttcgcggtat
     1741 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
     1801 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     1861 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     1921 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     1981 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     2041 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     2101 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     2161 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     2221 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     2281 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     2341 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     2401 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     2461 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     2521 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     2581 ggatatctca gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     2641 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     2701 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     2761 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2821 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2881 atgtaagtta gctcactcat taggcacaat tctcatgttt gacagcttat catcgactgc
     2941 acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag
     3001 gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt
     3061 tttgcgccga catcataacg gttctggcaa atattctgaa atgagctgtt gacaattaat
     3121 catcggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagcc
     3181 agtccgttta ggtgttttca cgagcacttc accaacaagg accatagcat atgaaaatcg
     3241 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
     3301 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
     3361 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
     3421 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
     3481 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
     3541 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
     3601 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
     3661 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
     3721 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
     3781 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac
     3841 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag
     3901 cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg
     3961 gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga
     4021 gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact
     4081 atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag
     4141 cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc actatggaaa
     4201 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg
     4261 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag
     4321 acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga
     4381 tcgagggaag gatcacaagt ttgtacaaaa aagcaggctt aatgtcggtt gtcgaagaag
     4441 ccactgacga cgcgctctac ccgattgccg tgctcattga cgagctgcgc aacgaagatg
     4501 tcacgttgcg actgaacagc attcgcaaac tctccacaat cgctttggcg ttgggtgtgg
     4561 agcgcacacg caacgagctc atccaatttt tgaccgacac gatttacgac gaagatgagg
     4621 ttctgcttgt gctcgccgag caactcggaa atttcactcc attggtcggt ggtccagatc
     4681 atgttcattg tcttctcctt ccacttgaga acttggccac agtcgaggag accgtggtcc
     4741 gtgacaaagc cgttgagagt ctccgcaaaa tcgccgacaa gcacagctcg gcctcattgg
     4801 aggagcattt cgtgccgatg ctcagacgtt tggccactgg agactggttc acatcccgta
     4861 cctcggcttg tgggcttttc tctgttgttt atccacgtgt cagtccagct atcaagtcgg
     4921 aattgaagag catgttccga accctgtgcc gtgatgacac cccaatggtt cgccgtgctg
     4981 ctgctgcaaa gctcggagag ttcgcaaagg tgtttgagaa gactgccgtc atcgagggtc
     5041 tccactccag tctcactgat cttcatgttg atgagcaaga ttctgttcgt ttgctcactg
     5101 tcgagtcagc catcgctttc ggaactctcc tcgataaggc taacaagaag aaactcatcg
     5161 agccaattct cattgaattg ttcgatgaca agtcgtggag agttcgttat atggttgccg
     5221 agaagcttat cgagattcag aatgtcctcg gtgaggatat ggataccaca catctcgtta
     5281 acatgtacac gaaccttctc aaggatccag agggtgaggt tagatgtgct gccactcaga
     5341 gacttcagga attcgctctc aacttgccag aggacaagcg tcagaacatt atctgcaact
     5401 cactgctcaa cgttgccaag gaattggtta ctgatgggaa tcagctcgtc aagtcggaac
     5461 tcgccggtgt catcatggga cttgctccac tgattggcaa ggaacaaact gtctctgagc
     5521 ttctcccaat ctacatgcag ttgcttaacg atcaaacccc agaagttcgc ctcaatatta
     5581 tttccagtct tgataaggtc aatgaagtga tcggagcagc tcaattgtcc acatccctct
     5641 tgccagccat cgttggattg gccgaggatg gaaaatggag agttcgactc gcaattgttc
     5701 aattcatgcc acttcttgct tcccagttgg gacaagaatt cttcgatgag aagttgcttc
     5761 cgctttgttt gaactggctt accgaccatg tgttctcaat ccgcgaggca tcgaccctca
     5821 tcatgaagga actgacacag aagtttggag gacaatgggc tagcacgaat attgtaccaa
     5881 aaatgcagaa acttcagaag gacaccaact acctgcagag aatgacatgc ttgttctgtt
     5941 tgaatacact cagtgaggcg atgactcagg agcagattct gaaggagatt atgccaattg
     6001 tgaaagatct tgtcgaggat gacgtgccga atgtcagatt caacgccgca aagagtttga
     6061 agcgaattgg aaagaacttg accccaagta cattgacgtc cgaggtgaag ccactgctcg
     6121 agaagctcgg aaaagattca gactttgacg ttcgatactt ttcagaggaa gccaagaact
     6181 cactcggctt gtaatcgaac atatcgctag tcaaattgga atctctacat cttccacttc
     6241 tcaatcggac ccagctttct tgtacaaagt ggtgatattt cagaattcgg atcctctaga
     6301 gtcgacctgc aggcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6361 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6421 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6481 cagcttggct gttttggcgg atgagataag attttcagcc tgatacagat taaatcagaa
     6541 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     6601 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     6661 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     6721 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     6781 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     6841 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     6901 tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6961 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7021 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7081 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7141 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgtttcccaa
     7201 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     7261 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7321 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7381 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7441 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7501 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7561 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7621 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7681 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7741 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7801 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7861 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaccc cggttgataa
     7921 tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat atttaaattg taaacgttaa
     7981 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     8041 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     8101 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     8161 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg
     8221 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     8281 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     8341 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     8401 tgcgccgcta cagggcgcgt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8461 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8521 aaggatcttc
//
