LOCUS       sequence-260221-        8129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of Akt and ERK activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Chavez-Abiega S, Gronloh MLB, Gadella TWJ, Bruggeman FJ, Goedhart J
  TITLE     Single-cell imaging of ERK and Akt activation dynamics and 
            heterogeneity induced by G-protein-coupled receptors.
  JOURNAL   J Cell Sci. 2022 Mar 15;135(6):jcs259685. doi: 10.1242/jcs.259685. 
            Epub 2022 Mar 11.
  PUBMED    35107584
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2022-03-15"; volume: "135(6):jcs259685. doi"; pages: "
            10.1242/jcs.259685. Epub 2022 Mar 11"
COMMENT     Sequence Label: 
            H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..8129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1042..1051
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1048..1740
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELY"
     primer_bind     complement(1181..1199)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1449..1468
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1759..1815
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      3022..3031
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3028..3030,3031..3033,3034..3741)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(3073..3094)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3334..3353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3681..3702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3760..3816
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      4078..4087
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4084..4794
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4339..4348
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4917..5038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4954..4973)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5008..5027
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5045..5500)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5182..5203)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5394..5413
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5527..5631
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5633..5990
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5633..5824
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5656..5676)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5841..5976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6025..6819
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6025..6819
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6079..6098)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6689..6708
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7007..7026)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7051..7098
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7427..8015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7916..7935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgatgt cgggacgcgg caagcaggga
      661 ggcaaagctc gcgccaaagc caagacccgc tcttctcgtg ccggtctcca gttccccgtg
      721 ggccgagtgc accgactgct ccgcaagggc aactatgctg agcgggtcgg ggccggcgcg
      781 ccggtgtacc tggcggcggt gctggagtac ctgactgccg agatcctgga gctggcgggc
      841 aacgccgccc gcgacaacaa gaagacccgc attatcccgc gccacttgca gctggccatc
      901 cgcaacgacg aggagctcaa caagctgctg ggcaaagtaa ccatcgctca gggtggtgtc
      961 ctgcccaaca tccaggctgt gctactgccc aagaagaccg agagtcacca caaggccaaa
     1021 ggcaaacggg atccaccggt cgccaccatg gtgagcaagg gcgaggcagt gatcaaggag
     1081 ttcatgcggt tcaaggtgca catggagggc tccatgaacg gccacgagtt cgagatcgag
     1141 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     1201 ggtggccccc tgcccttctc ctgggacatc ctgtcccctc agttcatgta cggctccagg
     1261 gccttcatca agcaccccgc cgacatcccc gactactata agcagtcctt ccccgagggc
     1321 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg ccgtgaccgt gacccaggac
     1381 acctccctgg aggacggcac cctgatctac aaggtgaagc tccgcggcac caacttccct
     1441 cctgacggcc ccgtaatgca gaagaagaca atgggctggg aagcgtccac cgagcggttg
     1501 taccccgagg acggcgtgct gaagggcgac attaagatgg ccctgcgcct gaaggacggc
     1561 ggccgctacc tggcggactt caagaccacc tacaaggcca agaagcccgt gcagatgccc
     1621 ggcgcctaca acgtcgaccg caagttggac atcacctccc acaacgagga ctacaccgtg
     1681 gtggaacagt acgaacgctc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     1741 caaaccatgg ggtcaggggc caccaacttc tctcttctga aacaagccgg cgacgtcgag
     1801 gaaaaccccg gacctatggc agaggcaccg gcttccccgg ccccgctctc tccgctcgaa
     1861 gtggagctgg acccggagtt cgagccccag agccgtccgc gatcctgtac gtggcccctg
     1921 caaaggccgg agctccaagc gagccctgcc aagccctcgg gggagacggc cgccgactcc
     1981 atgatccccg aggaggagga cgatgaagac gacgaggacg gcgggggacg ggccggctcg
     2041 gccatggcga tcggcggcgg cggcgggagc ggcacgctgg gctccgggct gctccttgag
     2101 gactcggccc gggtgctggc acccggaggg caagaccccg ggtctgggcc agccaccgcg
     2161 gcgggcgggc tgagcggggg tacacaggcg ctgctgcagc ctcagcaacc gctgccaccg
     2221 ccgcagccgg gggtggctgg gggctccggg cagccgagga aatgttcgtc gcggcggaac
     2281 gcctggggaa acctgtccta cgcggacctg atcacccgcg ccatcgagag ctccccggac
     2341 aaacggctca ctctgtccca gatctacgag tggatggtgc gttgcgtgcc ctacttcaag
     2401 gataagggcg acagcaacag ctctgccggc tggaagaact ccatccggcg caacctgtca
     2461 ctgcatagtc gattcatgcg ggtccagaat gagggaactg gcaagagctc ttggtggatc
     2521 atcaaccctg atggggggaa gagcggaaaa gccccccggc ggcgggctgt ctccatggac
     2581 aatagcaaca agtataccaa gagccgtggc cgcgcagcca agaagaaggc agccctgcag
     2641 acagcccccg aatcagctga cgacagtccc tcccagctct ccaagtggcc tggcgccccc
     2701 acgtcacgca gcagtgatga gctggatgcg tggacggact tccgttcacg caccaattct
     2761 aacgccagca cagtcagtgg ccgcctgtcg cccatcatgg caagcacaga gttggatgaa
     2821 gtccaggacg atgatgcgcc tctcgcgccc atgctctaca gcagctcagc cagcctgtca
     2881 ccttcagtaa gcaagccgtg cacggtggaa ctgccacggc tgactgatat ggcaggcacc
     2941 atgaatctga atgatgggct gactgaaaac ctcatggacg acctgctgga taacatcacg
     3001 ctcggcggcc gcataccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     3061 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgtcctgggg cgtgcagtgc
     3241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actactttag cgacaacgtc
     3481 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     3541 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     3661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3721 ctcggcatgg acgagctgta ccaaaccatg gggagcgggg ctaccaactt ctccctcctc
     3781 aagcaggcag gggacgtcga ggaaaatcct ggacccatga aaggccgcaa gcctcgcgat
     3841 ctggagttgc ctctcagccc tagcctcctg gggggacagg gccctgagcg cacacccggg
     3901 tccggcacct ctagtggttt gcaggctccg ggacctgccc tgtcaccctc caagcgcagc
     3961 ggcctggagg atcctgccac acccagcaaa aaaccaagga ccccctcagt cagctcgcgg
     4021 ttagagcgac tgacgctcca gtcttctttc caattcccgt ccggcggcat accggtcgcc
     4081 accatggtga gcaagggcga ggaggataac atggcctctc tcccagcgac acatgagtta
     4141 cacatctttg gctccatcaa cggtgtggac tttgacatgg tgggtcaggg caccggcaat
     4201 ccaaatgatg gttatgagga gttaaacctg aagtccacca agggtgacct ccagttctcc
     4261 ccctggattc tggtccctca tatcgggtat ggcttccatc agtacctgcc ctaccctgac
     4321 gggatgtcgc ctttccaggc cgccatggta gatggctccg gctaccaagt ccatcgcaca
     4381 atgcagtttg aagatggtgc ctcccttact gttaactacc gctacaccta cgagggaagc
     4441 cacatcaaag gagaggccca ggtgaagggg actggtttcc ctgctgacgg tcctgtgatg
     4501 accaactcgc tgaccgctgc ggactggtgc aggtcgaaga agacttaccc caacgacaaa
     4561 accatcatca gtacctttaa gtggagttac accactggaa atggcaagcg ctaccggagc
     4621 actgcgcgga ccacctacac ctttgccaag ccaatggcgg ctaactatct gaagaaccag
     4681 ccgatgtacg tgttccgtaa gacggagctc aagcactcca agaccgagct caacttcaag
     4741 gagtggcaaa aggcctttac cgatgtgatg ggcatggacg agctgtacaa gtaaagcggc
     4801 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4861 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4921 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4981 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     5041 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     5101 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     5161 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     5221 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5281 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5341 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5401 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5461 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     5521 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5581 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     5641 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5761 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5821 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5881 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     5941 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     6001 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6061 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6121 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6181 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     6241 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6301 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6361 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6421 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6481 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6541 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6601 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6661 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6721 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6781 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6841 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6901 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6961 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     7021 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     7081 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     7141 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     7201 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     7261 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7321 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7561 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8041 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8101 ctgtggataa ccgtattacc gccatgcat
//
