LOCUS       sequence-260246-        7900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Kv2.1in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Jorge BS, Campbell CM, Miller AR, Rutter ED, Gurnett CA, Vanoye CG, 
            George AL Jr, Kearney JA
  TITLE     Voltage-gated potassium channel KCNV2 (Kv8.2) contributes to 
            epilepsy susceptibility.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5443-8. doi: 
            10.1073/pnas.1017539108. Epub 2011 Mar 14.
  PUBMED    21402906
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1017539108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-29- 29"; volume: "108"; issue: "13"; pages: "5443-8"
COMMENT     Sequence Label: pIRES2-DsRed-MST-Kv2.1
FEATURES             Location/Qualifiers
     source          1..7900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    join(2939..3513,3514..3516,3517..3525)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3106..3123)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3333..3352
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3526..4203
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(3656..3674)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3706..3726)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3924..3943
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4118..4141
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4324..4445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4361..4380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4415..4434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4452..4907)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4589..4610)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4801..4820
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4934..5038
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5040..5397
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5040..5231
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5063..5083)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5248..5383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5432..6226
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5432..6226
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5486..5505)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6096..6115
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6414..6433)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6458..6505
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6834..7422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7323..7342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7597..7900
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gtaacggccg
      241 ccagtgtgct ggaattcgcc cttgggggcg gccggatcag cgatgccggc gggcatgacg
      301 aagcatggct cccgctccac cagctcgctg ccgcccgagc ccatggagat cgtgcgcagc
      361 aaggcgtgct ctcggcgggt ccgcctcaac gtcggggggc tggcgcacga ggtactctgg
      421 cgtaccctgg accgcctgcc ccgcacgcgg ctgggcaagc tccgcgactg caacacgcac
      481 gactcgctgc tcgaggtgtg cgatgactac agcctcgacg acaacgagta cttctttgac
      541 cgccacccgg gcgccttcac ctccatcctc aacttctacc gcactgggcg actgcacatg
      601 atggaggaga tgtgcgcgct cagcttcagc caagagctcg actactgggg catcgacgag
      661 atctacctgg agtcctgctg ccaggcccgc taccaccaga agaaagagca gatgaacgag
      721 gagctcaagc gtgaggccga gaccctacgg gagcgggaag gcgaggagtt cgataacacg
      781 tgctgcgcag agaagaggaa aaaactctgg gacctactgg agaagcccaa ttcctctgtg
      841 gctgccaaga tccttgccat aatttccatc atgttcatcg tcctctccac cattgccctg
      901 tccctcaaca cgctgcctga gctacagagc ctcgatgagt tcggccagtc cacagacaac
      961 ccccagctgg cccacgtgga ggccgtgtgc atcgcatggt tcaccatgga gtacctgctg
     1021 aggttcctct cctcgcccaa gaagtggaag ttcttcaagg gcccactcaa tgccattgac
     1081 ttgttggcca ttctgccata ctatgtcacc attttcctca ccgaatccaa caagagcgtg
     1141 ctgcaattcc agaatgtccg ccgcgtggtc cagatcttcc gcatcatgcg aattctccgc
     1201 atccttaagc ttgcacgcca ctccactggc ctccagtctc tgggcttcac tttgcggagg
     1261 agctacaatg agttgggctt gctcatcctc ttccttgcca tgggcattat gatcttctcc
     1321 agccttgtct tctttgctga gaaggatgag gacgacacca agttcaaaag catcccagcc
     1381 tctttctggt gggccaccat caccatgact actgttgggt atggagacat ctaccccaag
     1441 actctcctgg ggaaaattgt tgggggactc tgctgcattg caggagtcct ggtgattgct
     1501 cttcccatcc ccatcatcgt caataacttc tctgagttct ataaggagca gaagagacag
     1561 gagaaagcaa tcaaacggcg agaggctctg gagagagcca agaggaatgg cagcatcgta
     1621 tccatgaaca tgaaggatgc ttttgcccgg agcattgaga tgatggacat tgtggttgag
     1681 aaaaatgggg agaatatggg taagaaagac aaagtacaag ataaccactt gtctcctaac
     1741 aaatggaaat ggacaaagag gacactgtct gaaaccagct caagtaagtc ctttgaaacc
     1801 aaggaacagg gatcccctga aaaagccaga tcgtcttcta gtcctcagca cctgaacgtt
     1861 cagcagttgg aagacatgta caataagatg gccaagaccc aatcccaacc catcctcaat
     1921 accaaggagt cagcagcaca gagcaaacca aaggaagaac ttgaaatgga gagtatcccc
     1981 agccccgtag cccctctgcc cactcgcaca gaaggggtca ttgacatgcg aagtatgtca
     2041 agcattgata gtttcattag ctgtgccaca gacttccctg aggccaccag attctcccac
     2101 agccctttga catcactccc cagcaagact gggggcagca cagccccaga agtgggctgg
     2161 cggggagctc tgggtgccag tggtggtagg tttgtggagg ccaaccccag ccctgatgcc
     2221 agccagcact ctagtttctt catcgagagc cccaagagtt ccatgaaaac taacaaccct
     2281 ttgaagctcc gagcacttaa agtcaacttc atggagggtg accccagtcc actcctcccc
     2341 gttctaggga tgtaccatga ccctctcagg aaccggggga gtgccgcggc tgctgtcgct
     2401 ggactggagt gtgccacgct tttggacaag gctgtgctga gcccagagtc ctccatctac
     2461 accacagcaa gtgctaagac acccccccgg tctcctgaga aacacacagc aatagcgttc
     2521 aactttgagg cgggtgtcca ccagtacatt gacgcagaca cagatgatga gggacagctg
     2581 ctctacagtg tggactccag cccccccaaa agcctccctg ggagcaccag tccgaagttc
     2641 agcacgggga caagatcgga gaaaaaccac tttgaaagct cccctttacc cacctcccct
     2701 aagttcttaa ggcagaactg tatttactcc acagaagcat tgactggaaa aggccccagt
     2761 ggtcaggaaa agtgcaaact tgagaaccac atctcccctg acgtccgtgt gttgccaggg
     2821 ggaggagccc atggaagcac acgagatcag agcatctgaa ctgccctgcc taagggcgaa
     2881 ttctgcagat atccatcaca ctggcggccg ctcgacggta ccgcgggccc gggatccgcc
     2941 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     3001 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     3061 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     3121 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     3181 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     3241 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     3301 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     3361 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca
     3421 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     3481 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggc ctcctccgag
     3541 gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
     3601 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     3661 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     3721 cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg
     3781 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     3841 accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc
     3901 ggcgtgaact tcccctccga cggccccgta atgcagaaga agactatggg ctgggaggcc
     3961 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca caaggccctg
     4021 aagctgaagg acggcggcca ctacctggtg gagttcaagt ctatctacat ggccaagaag
     4081 cccgtgcagc tgcccggcta ctactacgtg gactccaagc tggacatcac ctcccacaac
     4141 gaggactaca ccatcgtgga gcagtacgag cgcgccgagg gccgccacca cctgttcctg
     4201 tagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     4261 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     4321 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4381 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4441 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4501 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4561 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4621 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4681 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4741 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4801 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4861 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     4921 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4981 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     5041 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5101 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5221 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5281 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5341 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     5401 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5461 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5521 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5581 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     5641 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5701 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5761 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5821 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5881 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     5941 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6001 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6061 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6121 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6181 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6241 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6301 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6361 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     6421 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     6481 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     6541 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     6601 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     6661 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     6721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6961 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7501 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
     7561 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     7621 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     7681 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     7741 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     7801 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     7861 ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
