LOCUS       sequence-260253-       11912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Davis MI, Bennett MJ, Thomas LM, Bjorkman PJ
  TITLE     Crystal structure of prostate-specific membrane antigen, a tumor 
            marker and peptidase.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Apr 26. 102(17):5981-6.
  PUBMED    15837926
REFERENCE   2  (bases 1 to 11912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Apr 26. 102(17):5981-6."
COMMENT     Sequence Label: pAcGP67A-PSMA
FEATURES             Location/Qualifiers
     source          1..11912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1..828
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             1..125
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(163..768)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        832..923
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     848..865
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     1020..1079
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             1089..1106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1116..1127
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_recomb     3751..4456
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3839..4211
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3868..4456)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(4890..4907)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6124..6140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6124..6140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6137..6159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6148..6164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6172..6178,6179..6196,6197..6202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6217..6238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6355..6372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6526..7114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6606..6625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7285..8076,8077..8145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7908..7927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8146..8250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8318..8336
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8374..8396)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8496..8515
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8709..8731
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8723..8740
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8724..8740
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
       61 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      121 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      181 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      241 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      301 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      361 ctcctcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      421 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      481 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      541 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      601 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      661 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      721 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      781 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tatcatggag
      841 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
      901 ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg
      961 gatctatgct actagtaaat cagtcacacc aaggcttcaa taaggaacac acaagcaaga
     1021 tggtaagcgc tattgtttta tatgtgcttt tggcggcggc ggcgcattct gcctttgcgg
     1081 cggatccgca tcatcatcat catcacagca gcggcatcga gggacgtggt aaatcctcca
     1141 atgaagctac taacattact ccaaagcata atatgaaagc atttttggat gaattgaaag
     1201 ctgagaacat caagaagttc ttatataatt ttacacagat accacattta gcaggaacag
     1261 aacaaaactt tcagcttgca aagcaaattc aatcccagtg gaaagaattt ggcctggatt
     1321 ctgttgagct agctcattat gatgtcctgt tgtcctaccc aaataagact catcccaact
     1381 acatctcaat aattaatgaa gatggaaatg agattttcaa cacatcatta tttgaaccac
     1441 ctcctccagg atatgaaaat gtttcggata ttgtaccacc tttcagtgct ttctctcctc
     1501 aaggaatgcc agagggcgat ctagtgtatg ttaactatgc acgaactgaa gacttcttta
     1561 aattggaacg ggacatgaaa atcaattgct ctgggaaaat tgtaattgcc agatatggga
     1621 aagttttcag aggaaataag gttaaaaatg cccagctggc aggggccaaa ggagtcattc
     1681 tctactccga ccctgctgac tactttgctc ctggggtgaa gtcctatcca gacggttgga
     1741 atcttcctgg aggtggtgtc cagcgtggaa atatcctaaa tctgaatggt gcaggagacc
     1801 ctctcacacc aggttaccca gcaaatgaat atgcttatag gcgtggaatt gcagaggctg
     1861 ttggtcttcc aagtattcct gttcatccaa ttggatacta tgatgcacag aagctcctag
     1921 aaaaaatggg tggctcagca ccaccagata gcagctggag aggaagtctc aaagtgccct
     1981 acaatgttgg acctggcttc actggaaact tttccacaca aaaagtcaag atgcacatcc
     2041 actctaccaa tgaagtgacg agaatttaca atgtgatagg tactctcaga ggagcagtgg
     2101 agccagacag atatgtcatt ctgggaggtc accgggactc atgggtgttt ggtggtattg
     2161 accctcagag tggagcagct gttgttcatg aaattgtgag gagctttgga acactgaaaa
     2221 aggaagggtg gagacctaga agaacaattt tgtttgcaag ctgggatgca gaagaatttg
     2281 gtcttcttgg ttctactgag tgggcagagg agaattcaag actccttcaa gagcgtggcg
     2341 tggcttatat taatgctgac tcatctatag aaggaaacta cactctgaga gttgattgta
     2401 caccgctgat gtacagcttg gtacacaacc taacaaaaga gctgaaaagc cctgatgaag
     2461 gctttgaagg caaatctctt tatgaaagtt ggactaaaaa aagtccttcc ccagagttca
     2521 gtggcatgcc caggataagc aaattgggat ctggaaatga ttttgaggtg ttcttccaac
     2581 gacttggaat tgcttcaggc agggcacggt atactaaaaa ttgggaaaca aacaaattca
     2641 gcggctatcc actgtatcac agtgtctatg aaacatatga gttggtggaa aagttttatg
     2701 atccaatgtt taaatatcac ctcactgtgg cccaggttcg aggagggatg gtgtttgagc
     2761 tagccaattc catagtgctc ccttttgatt gtcgagatta tgctgtagtt ttaagaaagt
     2821 atgctgacaa aatctacagt atttctatga aacatccaca ggaaatgaag acatacagtg
     2881 tatcatttga ttcacttttt tctgcagtaa agaattttac agaaattgct tccaagttca
     2941 gtgagagact ccaggacttt gacaaaagca acccaatagt attaagaatg atgaatgatc
     3001 aactcatgtt tctggaaaga gcatttattg atccattagg gttaccagac aggccttttt
     3061 ataggcatgt catctatgct ccaagcagcc acaacaagta tgcaggggag tcattcccag
     3121 gaatttatga tgctctgttt gatattgaaa gcaaagtgga cccttccaag gcctggggag
     3181 aagtgaagag acagatttat gttgcagcct tcacagtgca ggcagctgca gagactttga
     3241 gtgaagtagc ctaagcggcc gctgcagatc tgatcctttc ctgggacccg gcaagaacca
     3301 aaaactcact ctcttcaagg aaatccgtaa tgttaaaccc gacacgatga agcttgtcgt
     3361 tggatggaaa ggaaaagagt tctacaggga aacttggacc cgcttcatgg aagacagctt
     3421 ccccattgtt aacgaccaag aagtgatgga tgttttcctt gttgtcaaca tgcgtcccac
     3481 tagacccaac cgttgttaca aattcctggc ccaacacgct ctgcgttgcg accccgacta
     3541 tgtacctcat gacgtgatta ggatcgtcga gccttcatgg gtgggcagca acaacgagta
     3601 ccgcatcagc ctggctaaga agggcggcgg ctgcccaata atgaaccttc actctgagta
     3661 caccaactcg ttcgaacagt tcatcgatcg tgtcatctgg gagaacttct acaagcccat
     3721 cgtttacatc ggtaccgact ctgctgaaga ggaggaaatt ctccttgaag tttccctggt
     3781 gttcaaagta aaggagtttg caccagacgc acctctgttc actggtccgg cgtattaaaa
     3841 cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
     3901 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
     3961 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
     4021 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
     4081 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
     4141 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
     4201 caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga
     4261 cgtaaacacg ttaaataaag cttggacata tttaacatcg ggcgtgttag ctttattagg
     4321 ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc
     4381 catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt
     4441 tgagcttttt ggaattattt ctgattgcgg gcgtttttgg gcgggtttca atctaactgt
     4501 gcccgatttt aattcagaca acacgttaga aagcgatggt gcaggcggtg gtaacatttc
     4561 agacggcaaa tctactaatg gcggcggtgg tggagctgat gataaatcta ccatcggtgg
     4621 aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg
     4681 cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc acctcaacta ttgtactggt
     4741 ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg cgattttttt cgtttctaat
     4801 agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat
     4861 tggtggagcg ggcggcaatt cagacatcga tggtggtggt ggtggtggag gcgctggaat
     4921 gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt ctggtttagt
     4981 ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg
     5041 tctgcttcga ggcagcgctt ggggtggtgg caattcaata ttataattgg aatacaaatc
     5101 gtaaaaatct gctataagca ttgtaatttc gctatcgttt accgtgccga tatttaacaa
     5161 ccgctcaatg taagcaattg tattgtaaag agattgtctc aagctcgcac gccgataaca
     5221 agccttttca tttttactac agcattgtag tggcgagaca cttcgctgtc gtcgacgtac
     5281 atgtatgctt tgttgtcaaa aacgtcgttg gcaagcttta aaatatttaa aagaacatct
     5341 ctgttcagca ccactgtgtt gtcgtaaatg ttgtttttga taatttgcgc ttccgcagta
     5401 tcgacacgtt caaaaaattg atgcgcatca attttgttgt tcctattatt gaataaataa
     5461 gattgtacag attcatatct acgattcgtc atggccacca caaatgctac gctgcaaacg
     5521 ctggtacaat tttacgaaaa ctgcaaaaac gtcaaaactc ggtataaaat aatcaacggg
     5581 cgctttggca aaatatctat tttatcgcac aagcccacta gcaaattgta tttgcagaaa
     5641 acaatttcgg cgcacaattt taacgctgac gaaataaaag ttcaccagtt aatgagcgac
     5701 cacccaaatt ttataaaaat ctattttaat cacggttcca tcaacaacca agtgatcgtg
     5761 atggactaca ttgactgtcc cgatttattt gaaacactac aaattaaagg cgagctttcg
     5821 taccaacttg ttagcaatat tattagacag ctgtgtgaag cgctcaacga tttgcacaag
     5881 cacaatttca tacacaacga cataaaactc gaaaatgtct tatatttcga agcacttgat
     5941 cgcgtgtatg tttgcgatta cggattgtgc aaacacgaaa actcacttag cgtgcacgac
     6001 ggcacgttgg agtattttag tccggaaaaa attcgacacc acaactatgc acgttcgttt
     6061 gactggtacg ccgtcggcgt gttaacatac aagttgctaa ccggcggcca attcgtaatc
     6121 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6181 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6241 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6301 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6361 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6421 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6481 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6541 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6601 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6661 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6721 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6781 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6841 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6901 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6961 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7021 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7081 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7141 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7261 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7321 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7381 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7441 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7501 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7561 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7621 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7681 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7741 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7801 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7861 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7921 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7981 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8041 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8101 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8161 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8221 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8281 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     8341 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     8401 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     8461 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     8521 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     8581 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     8641 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     8701 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg gctcgtaaag
     8761 cgagttgaag gatcatattt agttgcgttt atgagataag attgaaagca cgtgtaaaat
     8821 gtttcccgcg cgttggcaca actatttaca atgcggccaa gttataaaag attctaatct
     8881 gatatgtttt aaaacacctt tgcggcccga gttgtttgcg tacgtgacta gcgaagaaga
     8941 tatgtggacc gcagaacaga tagtaaaaca aaaccctagt attggagcaa taatcgattt
     9001 aaccaacacg tctaaatatt atgatggtgt gcattttttg cgggcgggcc tgttatacaa
     9061 aaaaattcaa gtacctggcc agactttgcc gcctgaaagc atagttcaag aatttattga
     9121 cacggtaaaa gaatttacag aaaagtgtcc cggcatgttg gtgggcgtgc actgcacaca
     9181 cggtattaat cgcaccggtt acatggtgtg cagatattta atgcacaccc tgggtattgc
     9241 gccgcaggaa gccatagata gattcgaaaa agccagaggt cacaaaattg aaagacaaaa
     9301 ttacgttcaa gatttattaa tttaattaat attatttgca ttctttaaca aatactttat
     9361 cctattttca aattgttgcg cttcttccag cgaaccaaaa ctatgcttcg cttgctccgt
     9421 ttagcttgta gccgatcagt ggcgttgttc caatcgacgg taggattagg ccggatattc
     9481 tccaccacaa tgttggcaac gttgatgtta cgtttatgct tttggttttc cacgtacgtc
     9541 ttttggccgg taatagccgt aaacgtagtg ccgtcgcgcg tcacgcacaa caccggatgt
     9601 ttgcgcttgt ccgcggggta ttgaaccgcg cgatccgaca aatccaccac tttggcaact
     9661 aaatcggtga cctgcgcgtc ttttttctgc attatttcgt ctttcttttg catggtttcc
     9721 tggaagccgg tgtacatgcg gtttagatca gtcatgacgc gcgtgacctg caaatctttg
     9781 gcctcgatct gcttgtcctt gatggcaacg atgcgttcaa taaactcttg ttttttaaca
     9841 agttcctcgg ttttttgcgc caccaccgct tgcagcgcgt ttgtgtgctc ggtgaatgtc
     9901 gcaatcagct tagtcaccaa ctgtttgctc tcctcctccc gttgtttgat cgcgggatcg
     9961 tacttgccgg tgcagagcac ttgaggaatt acttcttcta aaagccattc ttgtaattct
    10021 atggcgtaag gcaatttgga cttcataatc agctgaatca cgccggattt agtaatgagc
    10081 actgtatgcg gctgcaaata cagcgggtcg ccccttttca cgacgctgtt agaggtaggg
    10141 cccccatttt ggatggtctg ctcaaataac gatttgtatt tattgtctac atgaacacgt
    10201 atagctttat cacaaactgt atattttaaa ctgttagcga cgtccttggc cacgaaccgg
    10261 acctgttggt cgcgctctag cacgtaccgc aggttgaacg tatcttctcc aaatttaaat
    10321 tctccaattt taacgcgagc cattttgata cacgtgtgtc gattttgcaa caactattgt
    10381 tttttaacgc aaactaaact tattgtggta agcaataatt aaatatgggg gaacatgcgc
    10441 cgctacaaca ctcgtcgtta tgaacgcaga cggcgccggt ctcggcgcaa gcggctaaaa
    10501 cgtgttgcgc gttcaacgcg gcaaacatcg caaaagccaa tagtacagtt ttgatttgca
    10561 tattaacggc gattttttaa attatcttat ttaataaata gttatgacgc ctacaactcc
    10621 ccgcccgcgt tgactcgctg cacctcgagc agttcgttga cgccttcctc cgtgtggccg
    10681 aacacgtcga gcgggtggtc gatgaccagc ggcgtgccgc acgcgacgca caagtatctg
    10741 tacaccgaat gatcgtcggg cgaaggcacg tcggcctcca agtggcaata ttggcaaatt
    10801 cgaaaatata tacagttggg ttgtttgcgc atatctatcg tggcgttggg catgtacgtc
    10861 cgaacgttga tttgcatgca agccgaaatt aaatcattgc gattagtgcg attaaaacgt
    10921 tgtacatcct cgcttttaat catgccgtcg attaaatcgc gcaatcgagt caagtgatca
    10981 aagtgtggaa taatgttttc tttgtattcc cgagtcaagc gcagcgcgta ttttaacaaa
    11041 ctagccatct tgtaagttag tttcatttaa tgcaacttta tccaataata tattatgtat
    11101 cgcacgtcaa gaattaacaa tgcgcccgtt gtcgcatctc aacacgacta tgatagagat
    11161 caaataaagc gcgaattaaa tagcttgcga cgcaacgtgc acgatctgtg cacgcgttcc
    11221 ggcacgagct ttgattgtaa taagttttta cgaagcgatg acatgacccc cgtagtgaca
    11281 acgatcacgc ccaaaagaac tgccgactac aaaattaccg agtatgtcgg tgacgttaaa
    11341 actattaagc catccaatcg accgttagtc gaatcaggac cgctggtgcg agaagccgcg
    11401 aagtatggcg aatgcatcgt ataacgtgtg gagtccgctc attagagcgt catgtttaga
    11461 caagaaagct acatatttaa ttgatcccga tgattttatt gataaattga ccctaactcc
    11521 atacacggta ttctacaatg gcggggtttt ggtcaaaatt tccggactgc gattgtacat
    11581 gctgttaacg gctccgccca ctattaatga aattaaaaat tccaatttta aaaaacgcag
    11641 caagagaaac atttgtatga aagaatgcgt agaaggaaag aaaaatgtcg tcgacatgct
    11701 gaacaacaag attaatatgc ctccgtgtat aaaaaaaata ttgaacgatt tgaaagaaaa
    11761 caatgtaccg cgcggcggta tgtacaggaa gaggtttata ctaaactgtt acattgcaaa
    11821 cgtggtttcg tgtgccaagt gtgaaaaccg atgtttaatc aaggctctga cgcatttcta
    11881 caaccacgac tccaagtgtg tgggtgaagt ca
//
