LOCUS       sequence-260254-        5829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of luciferase driven by eIF3B promoter region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Santasusagna S, Zhu S, Jawalagatti V, Carceles-Cordon M, Ertel A, 
            Garcia-Longarte S, Song WM, Fujiwara N, Li P, Mendizabal I, Petrylak
            DP, Kelly WK, Reddy EP, Wang L, Schiewer MJ, Lujambio A, Karnes J, 
            Knudsen KE, Cordon-Cardo C, Dong H, Huang H, Carracedo A, Hoshida Y,
            Rodriguez-Bravo V, Domingo-Domenech J
  TITLE     Master transcription factor reprograming unleashes selective 
            translation promoting castration resistance and immune evasion in 
            lethal prostate cancer.
  JOURNAL   Cancer Discov. 2023 Sep 7. doi: 10.1158/2159-8290.CD-23-0306.
  PUBMED    37676710
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2023 Sep 7. doi: 10.1158/2159-8290.CD-23-0306."
COMMENT     Sequence Label: pGL4-eIF3B
FEATURES             Location/Qualifiers
     source          1..5829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..92
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     41..60
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      1773..1782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1779..3431
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3475..3596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3486..3505)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3540..3559
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3844..3861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4015..4603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4095..4114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4803..5594,5595..5663))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5768..5816
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
       61 cagtgcaagt gcaggtgcca gaacatttct ctggcctaac tggccggtac ctgagctcgc
      121 tagcacaggg tctcgctatg ttgccctgac tggtcttgaa ctcttgggct caagtgatcc
      181 tcctgtcttg gcctcccaaa gtactgggaa gagaggtgtg agccaccgcg cagggcctag
      241 ccattttaat atgaagttta gtatacagtt cagtggcatc aagtacattc accatgttgt
      301 accatcacca ccgtcatctc caaaacgttc tcatcctccc aaattgaagg tccgtccccg
      361 ataaacactc cccactctcc accccagcct ctgggtacca cctgttttat ttatttattt
      421 aattttcgta gagatgaggg ctggcgtgcg gggggtggtg gtgctatgtt ggcaaggctg
      481 gtctccaact cctaagctca agcgatcttc ccacctcagc ctcccaaagt gctgggataa
      541 caggcgtgat ccactgggtt gttttctagg atgattaaga catcccccaa ccagtggttg
      601 tcctccactc cggcaaaaaa caagatagaa gccacatgca cccaatgccc gtcccgtgag
      661 cacccgcgca gagcgaaccg ctccggtccc tccggagtca gcctcgagct ccacgcggcg
      721 aggacccgcc cccgccccca cgtgacccgc gctgtcccgc gctgaaggcg gcggcctcgg
      781 atggactgaa ccactgtgaa agcccgaatg gggggaaaca ggcagtgacg gccgctaggg
      841 aaggactggg ggtaagcgat taaagaaact tccatacatt tttgtatcgc gccaggcagt
      901 cgtccttcta gtcgtgtggg ccctctctgg ctcgccccga attccttttc tattttgaag
      961 tagtctgtcc ttttgctcag tcagtaggcg agtccaagtg acagacacgg acagggggga
     1021 ctttagtaac gtaaactaaa cggtttccct agcgaaaggc tcagacgccc tgagcaatcg
     1081 cctgttgagt ctccactctt ccagggtatt aactgttgag ttagaggaca ttttcctagc
     1141 attccgggta ctaaaatcat ttcttgtccc cgccttcagc tccacaaata gttcagtccc
     1201 gccccgcagc gtccgacggg aaaggaaagg gggaggacgc catgttggac ggaaccatct
     1261 gctgccacac atggggtggt caatgctccc atgagtttac tgatgcacag aaacaacctt
     1321 gctgaatgga attaagcact ttcttgtcac aaatatatca gtttgatgat tttcgggcgc
     1381 ggtcttgtgg atgcgagtcc tctcagggtc gttcgtgagg ccccagggcg tgggtgcgcc
     1441 ccccgccccg cggccttggt gcggcctccc cgtcgcacgc acatggctgg gctgtagccg
     1501 tcgcggcgcg cggtgcggcc tgggagagtc ggaagcgcgg cggccgcgga gccctgcgag
     1561 taggcagcgt tgggcccatg caggacgcgg agaacgtggc ggtgcccgag gcggccgagg
     1621 agcgcgccga gcccggccag cagcagccgg ccgccgagcc gccgccagcc gaggggctgc
     1681 tgcggcccgc ggggcccggc gctccggagg ccgcggggac cgagagatct ggcctcggcg
     1741 gccaagcttg gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta
     1801 agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca
     1861 aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg
     1921 aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga
     1981 agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt
     2041 tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca
     2101 tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg
     2161 tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa
     2221 agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg
     2281 tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc
     2341 gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg
     2401 tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg
     2461 gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg
     2521 gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct
     2581 tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg
     2641 tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca
     2701 acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg
     2761 ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg
     2821 ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct
     2881 tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg
     2941 gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta
     3001 caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg
     3061 aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc
     3121 aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg
     3181 gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg
     3241 aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa
     3301 ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg
     3361 gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga
     3421 tcgccgtgta ataattctag agtcggggcg gccggccgct tcgagcagac atgataagat
     3481 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     3541 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     3601 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     3661 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg
     3721 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     3781 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     3841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4021 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4141 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4201 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4261 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4321 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4381 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4441 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4501 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4621 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4681 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4741 ctaaagtata tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt
     4801 ggttaccagt gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc
     4861 atagtggcct gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc
     4921 cccagcgcag caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg
     4981 aaccagccag cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc
     5041 cagtctatga gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga
     5101 agagttgtgg ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg
     5161 ttcaactctg gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat
     5221 gcagtcagct ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg
     5281 ctcatggtaa tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt
     5341 tccgtgaccg gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc
     5401 tgctcttgcc cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg
     5461 ctcatcatcg ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga
     5521 tccagttcga tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc
     5581 agcgtttcgg ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg
     5641 acacgaaaat gttggatgct catactcgtc ctttttcaat attattgaag catttatcag
     5701 ggttactagt acgtctctca aggataagta agtaatatta aggtacggga ggtattggac
     5761 aggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     5821 gatagtact
//
