LOCUS       sequence-260256-        5829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of luciferase driven by eIF3B promoter region mutated at 
            the E-box binding motif 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Santasusagna S, Zhu S, Jawalagatti V, Carceles-Cordon M, Ertel A, 
            Garcia-Longarte S, Song WM, Fujiwara N, Li P, Mendizabal I, Petrylak
            DP, Kelly WK, Reddy EP, Wang L, Schiewer MJ, Lujambio A, Karnes J, 
            Knudsen KE, Cordon-Cardo C, Dong H, Huang H, Carracedo A, Hoshida Y,
            Rodriguez-Bravo V, Domingo-Domenech J
  TITLE     Master transcription factor reprograming unleashes selective 
            translation promoting castration resistance and immune evasion in 
            lethal prostate cancer.
  JOURNAL   Cancer Discov. 2023 Sep 7. doi: 10.1158/2159-8290.CD-23-0306.
  PUBMED    37676710
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2023 Sep 7. doi: 10.1158/2159-8290.CD-23-0306."
COMMENT     Sequence Label: pGL4-eIF3B MBE3 mutant
FEATURES             Location/Qualifiers
     source          1..5829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..49
                     /note="synthetic polyadenylation signal"
     misc_feature    63..154
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     103..122
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      1835..1844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1841..3493
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3537..3658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3548..3567)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3602..3621
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3906..3923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4077..4665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4157..4176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4865..5656,5657..5725))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctctggccta actggccggt acctgagctc
      181 gctagcacag ggtctcgcta tgttgccctg actggtcttg aactcttggg ctcaagtgat
      241 cctcctgtct tggcctccca aagtactggg aagagaggtg tgagccaccg cgcagggcct
      301 agccatttta atatgaagtt tagtatacag ttcagtggca tcaagtacat tcaccatgtt
      361 gtaccatcac caccgtcatc tccaaaacgt tctcatcctc ccaaattgaa ggtccgtccc
      421 cgataaacac tccccactct ccaccccagc ctctgggtac cacctgtttt atttatttat
      481 ttaattttcg tagagatgag ggctggcgtg cggggggtgg tggtgctatg ttggcaaggc
      541 tggtctccaa ctcctaagct caagcgatct tcccacctca gcctcccaaa gtgctgggat
      601 aacaggcgtg atccactggg ttgttttcta ggatgattaa gacatccccc aaccagtggt
      661 tgtcctccac tccggcaaaa aacaagatag aagccacatg cacccaatgc ccgtcccgtg
      721 agcacccgcg cagagcgaac cgctccggtc cctccggagt cagcctcgag ctccacgcgg
      781 cgaggacccg cccccgcccc aaaaaaaccc gcgctgtccc gcgctgaagg cggcggcctc
      841 ggatggactg aaccactgtg aaagcccgaa tggggggaaa caggcagtga cggccgctag
      901 ggaaggactg ggggtaagcg attaaagaaa cttccataca tttttgtatc gcgccaggca
      961 gtcgtccttc tagtcgtgtg ggccctctct ggctcgcccc gaattccttt tctattttga
     1021 agtagtctgt ccttttgctc agtcagtagg cgagtccaag tgacagacac ggacaggggg
     1081 gactttagta acgtaaacta aacggtttcc ctagcgaaag gctcagacgc cctgagcaat
     1141 cgcctgttga gtctccactc ttccagggta ttaactgttg agttagagga cattttccta
     1201 gcattccggg tactaaaatc atttcttgtc cccgccttca gctccacaaa tagttcagtc
     1261 ccgccccgca gcgtccgacg ggaaaggaaa gggggaggac gccatgttgg acggaaccat
     1321 ctgctgccac acatggggtg gtcaatgctc ccatgagttt actgatgcac agaaacaacc
     1381 ttgctgaatg gaattaagca ctttcttgtc acaaatatat cagtttgatg attttcgggc
     1441 gcggtcttgt ggatgcgagt cctctcaggg tcgttcgtga ggccccaggg cgtgggtgcg
     1501 ccccccgccc cgcggccttg gtgcggcctc cccgtcgcac gcacatggct gggctgtagc
     1561 cgtcgcggcg cgcggtgcgg cctgggagag tcggaagcgc ggcggccgcg gagccctgcg
     1621 agtaggcagc gttgggccca tgcaggacgc ggagaacgtg gcggtgcccg aggcggccga
     1681 ggagcgcgcc gagcccggcc agcagcagcc ggccgccgag ccgccgccag ccgaggggct
     1741 gctgcggccc gcggggcccg gcgctccgga ggccgcgggg accgagagat ctggcctcgg
     1801 cggccaagct tggcaatccg gtactgttgg taaagccacc atggaagatg ccaaaaacat
     1861 taagaagggc ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca
     1921 caaagccatg aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat
     1981 cgaggtggac attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat
     2041 gaagcgctat gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca
     2101 gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga
     2161 catctacaac gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt
     2221 cgtgagcaag aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca
     2281 aaagatcatc atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt
     2341 cgtgacttcc catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga
     2401 ccgggacaaa accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg
     2461 cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt
     2521 cggcaaccag atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt
     2581 cggcatgttc accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg
     2641 cttcgaggag gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct
     2701 ggtgcccaca ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag
     2761 caacttgcac gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt
     2821 ggccaaacgc ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag
     2881 cgccattctg atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc
     2941 cttcttcgag gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg
     3001 cggcgagctg tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc
     3061 tacaaacgct ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga
     3121 cgaggacgag cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta
     3181 ccaggtagcc ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc
     3241 cggggtcgcc ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct
     3301 ggaacacggt aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac
     3361 aaccgccaag aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac
     3421 cggcaagttg gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa
     3481 gatcgccgtg taataattct agagtcgggg cggccggccg cttcgagcag acatgataag
     3541 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3601 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3661 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     3721 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct
     3781 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     3841 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc
     3901 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3961 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4021 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4081 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4141 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4201 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4261 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4321 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4381 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4441 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4501 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4561 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4621 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4681 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4741 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4801 atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta aaccactgca
     4861 gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta tttcgttcgt
     4921 ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag
     4981 gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa
     5041 tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg tccgcctcca
     5101 tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc
     5161 gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc ggtatggctt
     5221 cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata ttatgaagaa
     5281 atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc gcggtgttgt
     5341 cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc gtaagatgct
     5401 tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa
     5461 gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt actttgaaag
     5521 tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg ccgctattga
     5581 gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct tttactttca
     5641 ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg
     5701 cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga agcatttatc
     5761 agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg
     5821 acaggccgc
//
