LOCUS       sequence-26026-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Inoue T, Heo WD, Grimley JS, Wandless TJ, Meyer T
  TITLE     An inducible translocation strategy to rapidly activate and inhibit 
            small GTPase signaling pathways.
  JOURNAL   Nat Methods. 2005 Jun . 2(6):415-8.
  PUBMED    15908919
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2005 Jun . 2(6):415-8."
COMMENT     Sequence Label: Lyn11-targeted FRB (LDR)
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           49..88
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             181..459
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             466..492
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctgttttgac ctccatagaa ganaccggga ccgatccagc ctccggggga tcttggtggc
       61 gtgaaactcc cgcacctctt cggccagcga attccagaag ccgccaccat gggatgtata
      121 aaatcaaaag ggaaagacag cgcgggagca gatagtgctg gtagtgctgg tagtgctggt
      181 atcctctggc atgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg
      241 gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg
      301 ggcccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag
      361 gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cctccaagcc
      421 tgggacctct attatcatgt gttccgacga atctcaaaga ctagttatcc gtacgacgta
      481 ccagactacg cataagaaaa gtgaggatcc tgagaacttc agggtgagtt tggggaccct
      541 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
      601 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt
      661 ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt
      721 ttctgtaact ttttcgttaa actttagctt gcatttg
//
