LOCUS       sequence-260263-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            C-terminal V5-miniTurbo for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-hcRed-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(156..197)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    199..223
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(910..934)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgnncntcga ttctgcatgc agcatcccca ctcttcagct cgccgattcg atccagcagg
       61 tactgattgg tggagtcgac cacaggcagg actgccacgc tcccgccgtc cagctgtccc
      121 agaatctgtt tagcgttcag cagcgggatg ctagcggtgc tgtccaggcc cagcaggggg
      181 ttggggatgg gcttgccgcc cactttgtac aagaaagttg gcaagttggc cttctcgggc
      241 aggtcgctgt accgggccac gctggcctcg tacagctcga agtactcgtc cttcttcttc
      301 cgcagcatct ggagccggat gtcggtgaag tggaagccgg gcatggtcag ggcgcgcacg
      361 gccttcttgc tccggtagct ggtgtagtgg tggcagatca ggtgccggtc gcccaccttc
      421 agggccatca cgttccggcc gcacagcacg ccgttctcgg ggtacaccac ctcggtgctg
      481 ggctcccagc cgccgctctt gttcttcatc acggggccgt cggcggggaa gttggtgccg
      541 tgcaccttca ccttgtagat caggcagttg ccctccaggc tggtgtcctg gtgggcggtc
      601 aggatgccgc cgtcctcgta ggtggtggtt ctctcccagg tgaagccctc ggggaagctc
      661 tgcttgaaga agtcggggat ctcggcggtg tggtgcacga aggtcctgct gccgtactcg
      721 cagcaggggg ccaggatgtc gaaggcgaag ggcagggggg cgccctcggt cacgtggatt
      781 ctcatgctct gggtgccggc gaaggggttg ccgtcgccct cgccctcgca cttgaagtag
      841 tggccgttca cggtgccctc catgtacatc ttgatgcgca tactctcctt cagcagccgc
      901 tcaccatggt gcccactttt ttgtacaaag ttgcgtctac aaaaagcaaa aacggccaga
      961 atttagcgga catttactag tctaacactg aaaattacat attgacccaa atgattacat
     1021 ttcaaaggtg cttaataaac ttcacaaaac acactcgcca accccgagcg catagtcaaa
     1081 tcggagcttc agctacttag aagaangtta cataaacgac nagactgacg cctctcttat
     1141 cctccctcac naaggtcggc cctgtcgaat cngaacctac ctagcnacct gnnctgcacg
     1201 aaaagcctnc gcaccttacg gcaagntgc
//
